| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,376,784 | G→A | A251T (GCG→ACG) | ycjR → | 3‑dehydro‑D‑guloside 4‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,376,784 | 0 | G | A | 94.4% | 41.6 / NA | 18 | A251T (GCG→ACG) | ycjR | 3‑dehydro‑D‑guloside 4‑epimerase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/8); minor base C (1/0); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
GGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGGATAACCATCGCTACCAGCCGGGCAGCGGCACCCTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAG > NC_000913/1376565‑1376959 | ggCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCATGCGCTGCATGACAACCGCGTCCTGCTCGGTCATGTGCATATTGCGGATAACCATCTCTACCAGCCGGTCAGCGGCACCCTGGATTTCCACGCGCGGTTTGAACAGCTGCTCACGGGTAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGc < 1:353323/247‑1 (MQ=255) aTTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGGATAACCATCGCTACCAGCCGGGCAGCGGCACCCTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTgn < 1:320109/251‑2 (MQ=255) atCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGGATAACCATCGCTACCAGCCGGGCAGCGGCACCCTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGa > 1:35062/1‑250 (MQ=255) agaagaTAACCGGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGGATAACCATCGCTACCAGCCGGGCAGCGGCACCCTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGGATCCGGGTGGAAGATTCTGTCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTg < 1:370533/251‑1 (MQ=255) aTGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGGATAACCATCGCTACCAGCCGGGCAGCGGCACCCTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTc < 1:136366/251‑1 (MQ=255) gTCATGTGCCTATTGCGGATAACCATCGCTACCAGCCGGGCAGCGGCACCCTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACGAGCTCTCCGCTGCGGGTCGCGATAATAgg > 1:386235/1‑241 (MQ=255) atTGCGGATAACCATCGCTACCAGCCGGGCAGCGGCACCCTGGATTTCCACGCGCTGTTGGAACAGCTGCGCGCGGATAACTTTCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGTGGAAGATTCTGTCCAGACGTACCGTGATTCGTTGGCCGGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGc < 1:437507/194‑1 (MQ=255) gATAACCATCGCTACCAGCCGGGCAGCGGCACCCTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCCGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGCAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTg > 1:291258/1‑164 (MQ=255) aCCATCGCTACCAGCCGGGCAGCGGCACCCTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAg > 1:13776/1‑242 (MQ=255) cAGCCGGGCAGCGGCACCCTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTactactn < 1:305260/251‑2 (MQ=255) ccTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGCTAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCCGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTgg > 1:174538/1‑251 (MQ=255) cTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATACCTATCAGGGCTATGTAGTGTCCGAAGGGCGTCTCAGGGCGGAAGATCCTGCCCAGACGAACCGTGATTCGTTGGCCTGGTTGCGt > 1:321984/1‑126 (MQ=255) gATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTACGCGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGCTAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGtac > 1:488987/1‑225 (MQ=255) ccACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGCCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCg > 1:413084/1‑163 (MQ=255) gAACAGCTGCGCGCGGATAACTATCAGGGCGATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCGGCCCAGACGTACCGTGATTCGTTCGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAATGCAATGGCAGGCTCCCCGCTTCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTGCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAg < 1:225922/251‑1 (MQ=255) cgcgGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCnctcn > 2:318758/1‑137 (MQ=255) cgcgGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACtggtggn < 1:318758/228‑2 (MQ=255) ggCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGACGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATCGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGc > 1:490011/1‑212 (MQ=255) | GGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGGATAACCATCGCTACCAGCCGGGCAGCGGCACCCTGGATTTCCACGCGCTGTTTGAACAGCTGCGCGCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAG > NC_000913/1376565‑1376959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |