Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,327,979	C→T	D127N (GAT→AAT)	btuR ←	cobinamide/cobalamin adenosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,327,979	0	C	T	100.0%	39.9 / NA	14	D127N (GAT→AAT)	btuR	cobinamide/cobalamin adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/9);  total (5/9)

CCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTG  >  NC_000913/1327798‑1328136                                                                                                                                                                                      |                                                                                                                                                              ccGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGCCAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATTAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTg                                                                                                                   >  1:432031/1‑226 (MQ=255)                                                 gCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATTAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACGCGCGGTTTTGTGTATCCCAGGTAAAGCGCGTTg                                           <  1:135572/250‑1 (MQ=255)                                                                         cAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATTAAGCAAAACCATATCCAGTGAGGNATCnnnnngcnnnnnnnnnn                                                                                                                   >  2:299479/1‑144 (MQ=255)                                                                         cAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATTAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTtgtn                                                                <  1:299479/205‑2 (MQ=255)                                                                                gACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCAGTAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAGGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTtgtg                                                                <  1:269724/198‑1 (MQ=255)                                                                                  cccGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATTAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTcgn                                                                         <  1:99675/187‑2 (MQ=255)                                                                                          aaTCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATTAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCATACTCGCGGTTTTGTTGATCCCAGGTAAAGCCCGTTGCCATCCCCTGAAACTCAACGCCATGTGGCTCCAGCAGATTg  <  1:378985/250‑1 (MQ=255)                                                                                             cACCGGCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATTAAGCAAAGCCATATCCAGGGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGGCTTCGCGGCATGCGGCGGTATCAGGCTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCGTCACCTGAAACTCAACGCCATGTGGCTCCAGCAGAtt   <  1:506402/246‑1 (MQ=255)                                                                                                                   tCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATTAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCTGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCcagc        <  1:349968/219‑1 (MQ=255)                                                                                                                            gCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACTCTATACGTCAGTTAATTAAGCAATACCCTCTCCAGTGAGGAATc                                                                                                                                    >  1:161955/1‑86 (MQ=255)                                                                                                                                 caccacTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATTAAGCAAAACCATATCCAGTGAgg                                                                                                                                        <  1:452066/77‑1 (MQ=255)                                                                                                                                                              aCGCCACCATATACGGCAGGTCATTAAGCAAAACCATAGCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCan                                                                                                        <  1:55981/80‑2 (MQ=255)                                                                                                                                                                   accaTATACGTCAGTTCATTAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTAGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTaa                                                    >  1:434283/1‑127 (MQ=255)                                                                                                                                                                    ccaTATACGTCAGTTCCTTAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGc                                                                                                           >  1:282803/1‑71 (MQ=255)                                                                                                                                                                                      |                                                                                                                                                              CCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATGTTGCCAGACTTCGCGGCAGGCGGCGGTATCAGACTCGCGGTTTTGTGTATCCCAGGTAAAGCCCGTTGCCATCACCTGAAACTCAACGCCATGTGGCTCCAGCAGATTG  >  NC_000913/1327798‑1328136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑