| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 480,295 | 2 bp→AA | coding (13‑14/219 nt) | hha ← | hemolysin expression‑modulating protein Hha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 480,295 | 0 | G | A | 93.8% | 36.8 / ‑5.8 | 16 | P5F (CCT→TTT) | hha | hemolysin expression‑modulating protein Hha |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/3); minor base T (1/0); total (13/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
| * | NC_000913 | 480,296 | 0 | G | A | 87.5% | 31.4 / NA | 16 | P5F (CCT→TTT) | hha | hemolysin expression‑modulating protein Hha |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (11/3); minor base C (2/0); total (13/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGT > NC_000913/480108‑480532 || tACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGACGGCATTTTACGGCTGAGTTAAATCCCGGCAGATCATTATCTGATCATTCGTATTTCTTTTTCTCGATAACAAGCTCCCGCCTGTCAATTGTCTGGCAACGACGCAAACCCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTACAAAGATa > 1:285696/1‑237 (MQ=255) ggTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATATGATAATTCGTATTTATTTTTCTAGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAACTTTTTCGGACATACTTCTAcc > 1:82320/1‑157 (MQ=255) cTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGAGTCAATTGTCTGGCAACGACGTAAACGCATTAAATTATCGGTTTTCGTTAAATATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTtcgc > 1:296834/1‑199 (MQ=255) gTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCAt > 1:30351/1‑250 (MQ=255) gTTCATTATCTGATAATTCGTATTTATTTTTATCGATAACACGCTCCAGCGTGTCAATTGTCTGGCACCGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACa > 1:176844/1‑149 (MQ=255) cTGATAATTCGTATTTATTTTTCTCGCTAACACGCTCCAGAGTGTCAATTGACTGGCACAGAAGTAAACTCATTAAATACTCGGTTTTCGTTAACAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCGACCAAGATAACCTCGCAGCATTCTCATTCGTCGCATTGACAAAACAACGGAATAGTCAATTACCTGACTTCAGCCATTTTTTAAGATCCTGCATATTAATAACATAACTACTGa > 1:376886/1‑250 (MQ=255) ttatttTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACTGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgtn < 1:56184/250‑2 (MQ=255) ntcGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAACCTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGACCAACATAAAGGTGTCATcc > 1:45661/2‑248 (MQ=255) tcGATAACNCNNNCNNGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGNTTTTCGTTAAAAATTNTTNGGACNTACNTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACACAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTNAAg > 2:347216/1‑198 (MQ=255) tcGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCTGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGTATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAg < 1:347216/198‑1 (MQ=255) gtCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGATTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATg > 1:498880/1‑251 (MQ=255) tCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTAGGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATg < 1:41926/250‑1 (MQ=255) ncATTAACTAATCGGTTTTCGTTAAAACTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAAt > 1:130793/2‑120 (MQ=255) cATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCCTTTCTGACGATCCTGCATATTAATACCATAACTACTGCACAACATAAAGGTGTCATCCAGa > 1:296863/1‑203 (MQ=255) aaaTAATCGGTTTTAGTTAAAAATTTTTCGGACATACTTCTAACTATGGGTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTg > 1:24714/1‑100 (MQ=255) ggTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGATGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCCTATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGt > 1:376286/1‑251 (MQ=255) || TACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATACTTAATTTTGTAATTAAGT > NC_000913/480108‑480532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |