Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	451,013	G→A	P200S (CCC→TCC)	cyoA ←	cytochrome bo3 subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	451,013	0	G	A	100.0%	40.7 / NA	17	P200S (CCC→TCC)	cyoA	cytochrome bo3 subunit 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/7);  total (10/7)

GGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAT‑ACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATGAAGAAGGAGTTCATCACGGAGTTGGAGGTCACTTTGAAGTACACCGGAGTGTTCGCCGGGAAAGCGATTTCATTCACGGTAGCAATGCCCTGTTCCGGGTAGATGAAGAACCATTTCCAGTCCATGGAAACC  >  NC_000913/450786‑451218                                                                                                                                                                                                                                     |                                                                                                                                                                                                              ggCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAT‑ACCGTCATAAGTGCCGGATTCGTTGGCGATCAGATGCAgg                                                                                                                                                                                         >  1:179404/1‑250 (MQ=255)      aCAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCCAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGAGCCGGTGTTTCAATAGCTGTGAACTGCATGCCTGAGGAGCCCGGACCGCTGTAGCTAGCGGAGAT‑ACCGTCATAAGTGCCGGATTCGTTGGCGATCAGATGCAGGCGAGTc                                                                                                                                                                                   <  1:480457/251‑1 (MQ=255)                                                          cgcgGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAT‑ACCGTCATAAGTGCCGGATTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATGAAGAAgg                                                                                                                               >  1:83954/1‑251 (MQ=255)                                                                                             tGGTGTTCGGCGACTGCTCCGCTTGTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGATGCTCGGACCGGTGTAGCTGGCGGAGAT‑ACCGTCATAAGTGCCGTATTCGTTGGCGATCAGa                                                                                                                                                                                               <  1:186653/152‑1 (MQ=255)                                                                                                                     ttGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGTAGAT‑ACCGTCATAAGTGCCGGAGTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGTAATGAAGAATGAGTTCATCACGGAGTTGGAGGTCACTTTGAAGTACACCGGAGTGTTCGTCGGGAAAGc                                                                     <  1:276126/250‑1 (MQ=255)                                                                                                                     ttGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAT‑ACCGTCATAAGTGCCGGATTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATGAAGAAGGAGTTCATCACGGAGTTGGAGGTCACTTTGAAGTACACCGGAGTGTTCGCCGGGAAAGc                                                                     <  1:21599/250‑1 (MQ=255)                                                                                                                                          cggcgCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAACCCGGGCCGCTGTAGCTGGCGGAGCTCACC‑TCATGAGTGCAGGATTCGTTTGCGGTCAGATGGAGGCGAGTCTGCATCCCgggcc                                                                                                                                                                      >  1:336014/1‑129 (MQ=255)                                                                                                                                                          tGCAATAGCTTTGAACGTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAT‑ACCGTCATAAGTGCCGGATTCGtt                                                                                                                                                                                                         <  1:228773/81‑1 (MQ=255)                                                                                                                                                            cAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAT‑ACCGTCATAAGTGCCGGATTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATGAAGAAGGAGt                                                                                                                            >  1:6314/1‑156 (MQ=255)                                                                                                                                                                                        gCCCGGGCCGCTGTAGCTGGCGGAGAT‑CCCGACATACGTGCCGGATTCGTTGGCGATCCGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATGAAGAAGGAGTTCATCACGGAGTTGGAGGTCACTTTGAAGTACACCGGAGTGTTCGCCGGGAAAGCGATTTCATTCACGGTAGCAATGCCCTGTTCCGGGTAGATGAAGAACCATTTCCAGTCCATGGAAAcc  >  1:428581/1‑250 (MQ=255)                                                                                                                                                                                           cGGGCCGCTGTAGCTGGCGGAGAT‑ACCGTCATAATGGCCGGATTCGTTGGCGCTCAGAGGCAGGCGAGTCTGCATACCGGCCATTGCATAAACCTGGCTACCCACACGCCGAATGAAGTAGGAGTTCATCACGGAGTTGGa                                                                                                            <  1:426896/141‑1 (MQ=255)                                                                                                                                                                                               ccGCTGTCGCTGGCGGTGAT‑ACCGTCATAAATGCCGGATTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATGAAGAAGGAGTTCATCACGGAGTTGGATGTCACTTTGAAGTACACCGGAGTGTTCGCCGGGAAAGCGATTTCATTCACGGTAGCAATGCCCTGTTCCGGGTAGATGAAGAACCATTTCCAGTCCATg        >  1:421079/1‑237 (MQ=255)                                                                                                                                                                                                cGCTGTAGCTGNCGGCGAT‑ACCGTCATAAGTGCCGGATTCNTTgg                                                                                                                                                                                                       >  2:75926/1‑45 (MQ=255)                                                                                                                                                                                                 gCTGTAGCTGGCGGAGAT‑ACCGTCATAAGTGCCGGATTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGCATGAAGAAGGAGTTCATCACGGAGTTGGAGGTCACTTTGAAGTACACCGGAGTGTTCGCCGGGAAAGCGATTTCATTCACGGTa                                                     >  1:190558/1‑190 (MQ=255)                                                                                                                                                                                                                  t‑ACCGTCATAAGTGCCGGATTCGTTGGCGATCAGATGCAGGCGAGTCTGc                                                                                                                                                                                <  1:42115/50‑1 (MQ=255)                                                                                                                                                                                                                  t‑ACCGTCATAAGTGCCGGATTCGTTGGCGATCAGATGCAGGCGAGTCTGc                                                                                                                                                                                >  2:42115/1‑50 (MQ=255)                                                                                                                                                                                                                         cATAAGTGCCGGATTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATGAAGAAGGAGTTCATCACGGAGTTGGAGGTCACTTTGAAGTACACCGGAGTGTTCGCCGGGAAAGCGATTTCATTCACGGTAGCAATGCCCTGTTCCGGGTAGATGAAGAACCATTTCCAGTCCATg        >  1:424487/1‑212 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                              GGCAAACAAGTCTGGTTTCACGTTGGAGAAATATTCCACCTGGTTGTATTCGCTAGGCGCGGCCAGTTTTTCGAACGCAGCCATGTCAGACATGGTGTTCGGCGACTGCTTCGCTTTTGCGACCCACTGGTCGAATGCGGCGCGATCCGGTGTTGCAATAGCTTTGAACTTCATGCCTGAGAAGCCCGGGCCGCTGTAGCTGGCGGAGAT‑ACCGTCATAAGTGCCGGGTTCGTTGGCGATCAGATGCAGGCGAGTCTGCATACCGGCCATGGCATAAATCTGGCTACCCAGACGCGGAATGAAGAAGGAGTTCATCACGGAGTTGGAGGTCACTTTGAAGTACACCGGAGTGTTCGCCGGGAAAGCGATTTCATTCACGGTAGCAATGCCCTGTTCCGGGTAGATGAAGAACCATTTCCAGTCCATGGAAACC  >  NC_000913/450786‑451218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑