| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,052,074 | C→T | E82K (GAA→AAA) | ubiI ← | 2‑octaprenylphenol 6‑hydroxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,052,074 | 0 | C | T | 85.7% | 22.1 / ‑4.4 | 14 | E82K (GAA→AAA) | ubiI | 2‑octaprenylphenol 6‑hydroxylase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (5/7); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
ACATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATTTTCTCCCCAGGCGACCTGCTGTAATTCTGCGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCA > NC_000913/3051855‑3052291 | aCATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATTTTCTCCCCAGGCGACCTGCTGTAATTCTGCGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTGGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTTCATACCGTGATAACAGCTGGCCCTACgagag < 1:372970/251‑1 (MQ=255) cAGAGTGATATCTGAAGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATAACTGAATTTTAAACGATATGCCCACGACGGAAATCCCCCATGATTTGATCGTCAAACGAACTGTGAACAACGCTGTCTTTGTCCAACACTTTCATAACGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGAc > 1:82876/1‑192 (MQ=255) ncgcTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCACCGATATGCCCACGATGGCTATAGCCCATGCTTTGATCGTCAAAAGAAATGTGACCACAGCTGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGACTGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGAATTCGCCGCCAGAgg > 1:95634/2‑250 (MQ=255) gcgTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAAAGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTTCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCAGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGACACGCGCCGTTGTGGGGGTGCATTCGCCGCCCGAgg > 1:296949/1‑233 (MQ=255) nnntttnCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTTCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGcc < 2:298711/137‑1 (MQ=255) naaTTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTTCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGcc > 1:298711/2‑140 (MQ=255) caacgctctGCCCAAGATGGCTCTAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTTCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAAcgcg > 1:138817/9‑181 (MQ=255) cccAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTTCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACgg < 1:430375/227‑1 (MQ=255) ggCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTTCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTggcgg < 1:334226/144‑1 (MQ=255) cTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTTCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTgtggtgg < 1:249022/173‑1 (MQ=255) cGTCAAACGAAATGTGTCCAAAGCTGTCTTGGTCCCACGCTTCCATTCCTTGATAACAGCTGGCCCGACGAGAGAGAATGTCCTGCCAGACGCCAAGACg < 1:444214/100‑1 (MQ=255) cGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTTCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTgtggtggng < 1:395634/156‑1 (MQ=255) gtgtgcccACACTTTCGTACCGTTATAGCAGCTGGGCCTACGAGAGAGAATGTCCTGCCAGACGGCAAGACTGGTGAGTAATTTTTCGCTGGCGGCAt < 1:218809/93‑1 (MQ=255) cccACACTTTCATACCGTGATAACAGCTGGCCCTACGAGATAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGAGGTGCATTCGCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGATCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCca > 1:5281/1‑227 (MQ=255) | ACATGCTGCCATCTTTCAGCGTCAGGAAGGTTTCATTTTCTCCCCAGGCGACCTGCTGTAATTCTGCGGGGGCTAACAGAGTGATATCTGACGACTGATGCGCTTTGTTCCACAGCGCGTAGTGAATCACTGAATTTTCAACGATATGCCCAAGATGGCTATAGCCCATGCTTTGATCGTCAAACGAAATGTGACCAAAGCTGTCTTTGTCCCACACTTCCATACCGTGATAACAGCTGGCCCTACGAGAGAGAATGTCCTGCCAGACGCCAAGACGGGTGAGTAATTTTTCGCTGGCGGCATTGATAGCCGAAACGCGCAGTTGTGGTGGTGCATTCGCCGCCAGAGGTTCCTGTACGCGCTGCTCCAGTACGGCAACGCGTAAGCCGCTCCCCTGTAAGCCACAGGCAACCGCCAGCCCCACCATGCCGCCGCCA > NC_000913/3051855‑3052291 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |