Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 700,628 C→T G323S (GGT→AGT)  nagC ← DNA‑binding transcriptional dual regulator NagC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913700,6280CT100.0% 79.0 / NA 27G323S (GGT→AGT) nagCDNA‑binding transcriptional dual regulator NagC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/7);  total (20/7)

CTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACC  >  NC_000913/700392‑700848
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                            
cTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGCGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGAcgaccg                                                                                                                                                                                                                 >  1:195074/1‑250 (MQ=255)
           tACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCCGAACGTAACACCGGCAGATTAGTGCGAAACACCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAAtt                                                                                                                                                                                                     >  1:489018/1‑251 (MQ=255)
                         ncgcgTTTTACCAGCGCCAAAGCGCCGATTGCCGAGCGGGGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGcc                                                                                                                                                                                         >  1:146385/2‑249 (MQ=255)
                                    cAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGAAGAATAGTGAGCAACGCCTTCAGCGCCTGGGAATTGATGCAGCTTTCAATAGCAGGAAGCAGCACTTAATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGAAGGTTTTACTCAGATGACGACCGACATACTc                                                                                                                                                                                                         >  1:84731/1‑222 (MQ=255)
                                                nccGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACAGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTAAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATAGCCTTTGTTCGCGGCtt                                                                                                                                                                >  1:53510/2‑251 (MQ=255)
                                                       gCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGc                                                                                                                                                             >  1:435925/1‑247 (MQ=255)
                                                            gCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTAGTAATGCAGCTTTCAAGAGCAGGGAGCAGCGCTTGATCGGCTGCGGTGATTTTACCGGCAATAAGAAGTTGTTGCGGAGTAAAGAAGTTGGTTGCAATGGCGATTGTTTTACTCAGATGACGACCGACATan                                                                                                                                                                                                           <  1:48612/196‑2 (MQ=255)
                                                             cGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCg                                                                                                                                                                                              >  1:108680/1‑208 (MQ=255)
                                                              ggTGATCCAGCTCAGAACGTACGACCGGCAGATTACTGCGAAACGCCTTCCGCGCATGGGTATTAATGCAGCTTTAAATAGCAGGGAGCAGAACTTTCTTGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGCTGACGACCGCCATACTCAATTACTTCCGACGCCAGCCTATCGCCTTTGTTcgcagatcggaagagacacgtc                                                                                                                                                  >  1:451389/1‑232 (MQ=255)
                                                               gTGATCCATCTCAGAAAGTACCAACGGTAGATTAGAGCGAAACGCCTTCAGCGCCTGGGAATTAATGCCGCTATCAATAGCCGGGAGCAGCACTTTATCCGCTTAGGTGATTTCACCGGACATCACACTTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCCATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGATTTGCAGATAGTTTTg                                                                                                                                                  >  1:409406/1‑250 (MQ=255)
                                                                     cAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGCATTATTGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATCACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGc                                                                                                                                           >  1:365841/1‑251 (MQ=255)
                                                                                        ggCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCAcgcg                                                                                                                        >  1:29852/1‑251 (MQ=255)
                                                                                                                                       ttCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATg                                                                          <  1:203667/250‑1 (MQ=255)
                                                                                                                                        tCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCTATGGTTTTACTCAGATGACGACCGACATACTCAAATACTTCCGACGCCAGACTATCGTCTTTTTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTan                                                                                                              <  1:45593/213‑2 (MQ=255)
                                                                                                                                                   gAGCAGCACTTTCTCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGCTTAACTCAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTCCTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGTAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCCCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGc                                                               >  1:113461/1‑249 (MQ=255)
                                                                                                                                                     gcagccCTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTa                                                                                                               <  1:34726/199‑1 (MQ=255)
                                                                                                                                                     gcagcaCTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGAc                                                                                                                                                                                     >  1:385328/1‑129 (MQ=255)
                                                                                                                                                      cagcaCTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTcc                                                                                                                                   >  1:178903/1‑178 (MQ=255)
                                                                                                                                                        gcaCTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGAt                                                         >  1:277986/1‑250 (MQ=255)
                                                                                                                                                                         gTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCCGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCaa                                        >  1:116728/1‑250 (MQ=255)
                                                                                                                                                                                     gCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTcg                                                                                                                                                                      >  1:22002/1‑112 (MQ=255)
                                                                                                                                                                                      cAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGACTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTcc                                                                                                                                   >  1:487604/1‑146 (MQ=255)
                                                                                                                                                                                                  tnnGCGGATNNAATAAGTTGATAGCAATGACGATGGTTTTACTCAAATGACGGCCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTcg                                                                                                                                                                      <  2:22002/99‑1 (MQ=255)
                                                                                                                                                                                                              aTAAGTTGATAGCAATGGCGATGGTTTTACTCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATATTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGAcc  <  1:218476/251‑1 (MQ=255)
                                                                                                                                                                                                                      aTAGCNATGAANANNGTTTTACTCAGATGACCACNGACATACTCAATTACNTCCGNCGCCan                                                                                                                                                                                       >  2:200137/1‑61 (MQ=255)
                                                                                                                                                                                                                      aTAGCAATGGCGATGGTTTTACTCAGATGACTACCGACATACTCAATTACTTCCGACGCCAg                                                                                                                                                                                       <  1:200137/62‑1 (MQ=255)
                                                                                                                                                                                                                          cAATGGCGATGGTTTTAATCAGATGACGAACGACATAATCAATTACTTCAGACCCCCGACTATCGCCTTTGTTCGCGGCttt                                                                                                                                                               >  1:431716/1‑82 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                            
CTGGAGCAAAATACCGTTGAGCATGGCGCGTTTTACCAGCGCAAAAGCGCCGATTGCCGAGCGGTGATCCAGCTCAGAACGTACCACCGGCAGATTAGTGCGAAACGCCTTCAGCGCCTGGGTATTAATGCAGCTTTCAATAGCAGGGAGCAGCACTTTATCGGCTTCGGTGATTTCACCGGCAATAACAATTTTTTGCGGATTAAATAAGTTGATAGCAATGGCGATGGTTTTACCCAGATGACGACCGACATACTCAATTACTTCCGACGCCAGACTATCGCCTTTGTTCGCGGCTTTGCAGATAGTTTTGATGGTGCAGTCGTCCAGCGGCACGCGGCTCTGGTAGCCCTGCTTTAACAGATTCAACACCCGTTGTTCAATGGCAGCGTTGGCAGCGATAGTTTCCAGGCAGCCAAAGTTGCCGCAGTGGCAGCGTTCACCCAGCGGTTCGACC  >  NC_000913/700392‑700848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑