Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,084,903	T→G	N307T (AAC→ACC)	fdoG ←	formate dehydrogenase O subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,084,903	0	T	G	100.0%	38.8 / NA	16	N307T (AAC→ACC)	fdoG	formate dehydrogenase O subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/9);  total (7/9)

CCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAG  >  NC_000913/4084697‑4085116                                                                                                                                                                                                               |                                                                                                                                                                                                                      ccACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTc                                                                                                                                                                                                          <  1:108599/220‑1 (MQ=255)           cGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCgg                                                                                                                                                                                                        >  1:119706/1‑212 (MQ=255)                           ngntgnnacagngaGTTCCACACGCTGCGCGCATGTNGCAGCGTGGTACCGCGTTTGGCGAAGCCGTTNTNGTCCATTGCATAAGTCCAGGAGGAGTTATCGTACTTGCGTTTTTCCGCNTCGGAGCCGGNGAANNGGCCNTNTNCAAAGGCGTAAGCCTCACGCACGATCAGGNTGGCGGTGGTATAGGCTTCGGTGNNTTNNNGCNNGAAtt                                                                                                                                                                                      <  2:363771/201‑1 (MQ=255)                            gCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAgg                                                                                                                                                <  1:81385/251‑1 (MQ=255)                                                   cAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCGGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGTTACAGCAATACGc                                                                                                                                                        <  1:163226/220‑1 (MQ=255)                                                                            cgcgTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGGAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAg                                                                                                                                            <  1:37454/207‑1 (MQ=255)                                                                                      gAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAACGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGGACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTAc                                                                                      >  1:391465/1‑251 (MQ=255)                                                                                                                                     tGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAgn                                                                                                                                                               <  1:123918/131‑2 (MQ=255)                                                                                                                                                 ngtcgtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATACTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATc                           >  1:103989/2‑251 (MQ=255)                                                                                                                                                     gtAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGGTCAGGCTGGCGGTGGCATAGGCGTCGGCGTATTAGGGGTTGACTTTTTCATTGTTCAGCAGGAACAAGAATACGGCTGACAGGAAAGCAATGTAAAGCCCGGAACGAATCGGGGAATAGTAGTCAGCTAACGCCGACGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGccc                       >  1:222655/1‑251 (MQ=255)                                                                                                                                                             tGAACAGGCAATCTTCAAAGCCGTAATCCTCACGCACTATCAGGCTGGCGGGGGTATAGGCTTCGGTGTATTCACGGATGGATTTTTCATTGTTCCGCATGTACAGCAATACGCCTGACAGGAAAGCCATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTCCGCGTAAAGCGAGGATCGATCACAATCAACTTCGCGCCGTTTTGCATTTTGGCTTCCATAGcc                        >  1:445451/1‑242 (MQ=255)                                                                                                                                                                      cATCTTCAAAGCCGTAATCCTCACTCTCGTTCAGGCTGTCTGTGTTATAGGCTTCGTTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGc                                                                                                                                                               <  1:265775/98‑1 (MQ=255)                                                                                                                                                                           tCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAg  <  1:244976/250‑1 (MQ=255)                                                                                                                                                                            cAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGGGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTAcc                                                                                                >  1:401857/1‑155 (MQ=255)                                                                                                                                                                                       tCCTCACGCACGATCAGGCTGGCGGTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTCAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCg                          >  1:74257/1‑214 (MQ=255)                                                                                                                                                                                          tCACGCACGAGCAGGCTGGCGGTGGTAGAGGCTTCGGAGTATGCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAg                                                                                                                                                                <  1:192719/77‑1 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                      CCACATCTGGCGTGTAGCGGGAAACGTGCTGTTTCAGCAAGTTCCACACGCAGCGCGGATGTTGCAGCGTGGTATCGCGTTTGGCGAAGCCGTTTTCGTCCAGTTCATAAGTCCAGGAGGATTTATCGTACTTGCGTTTTTCCGCGTCGTAGCCGGTGAACAGGCCATCTTCAAAGCCGTAATCCTCACGCACGATCAGGCTGGCGTTGGTATAGGCTTCGGTGTATTCGCGGTTGAATTTTTCATTGTTCAGCAGGTACAGCAATACGCCTGACAGGAAAGCAATGTCAGTACCGGAACGAATAGGGGCATAGTAGTCAGCTACCGCCGCCGTACGCGTAAAGCGAGGATCGATCACAATCAGCTTCGCGCCGTTGTGAATTTTGGCTTCCATCGCCCAGCGGAACCCGACCGGGTGAG  >  NC_000913/4084697‑4085116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑