Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,127,608	G→A	G268D (GGC→GAC)	yceM →	putative oxidoreductase YceM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,127,608	0	G	A	100.0%	39.5 / NA	16	G268D (GGC→GAC)	yceM	putative oxidoreductase YceM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/5);  total (11/5)

AACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCG‑AACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATT  >  NC_000913/1127373‑1127849                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   aaCGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGACTGTGCGCGGCAc                                                                                                                                                                                                                                       <  1:427027/249‑1 (MQ=255) aaCGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGACTGGGCGCGGCAc                                                                                                                                                                                                                                       <  1:180608/249‑1 (MQ=255)        cTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGAGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGACTGTGCGCGGCACTTCAtt                                                                                                                                                                                                                                 >  1:142557/1‑248 (MQ=255)            ngAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGACTGTGCGCGGCACTTCATTGAAtgtg                                                                                                                                                                                                                          >  1:46940/2‑251 (MQ=255)                                 ccATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGACTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGc                                                                                                                                                                                                     <  1:8896/251‑1 (MQ=255)                                                              ccaccTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCGCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAACCCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGACTGTGCGCGGCACTTCATTGAAGGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCG‑AACAGGCCGTGCTGgcg                                                                                                                                                                       <  1:166964/251‑1 (MQ=255)                                                                                     ggAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGACTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGc                                                                                                                                                                                          >  1:29/1‑210 (MQ=255)                                                                                           cAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCACTCATCGACATTACGGCTATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTAAGCTTGAGCAACGTGGGTTTGTCGACTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCG‑AACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTg                                                                                                                                                >  1:409052/1‑245 (MQ=255)                                                                                                          cAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGAGTGAGGAGAGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGACTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCG‑AACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAAt                                                                                                                            >  1:333263/1‑250 (MQ=255)                                                                                                                                                   gcgcGAATGGCGTGAGGAGAGCGGGCAGGGCGTAGTGCCTAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGACTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCG‑AACAGGc                                                                                                                                                                                 >  1:208131/1‑156 (MQ=255)                                                                                                                                                                       ncgGGCAGGGCGTAGTGCATAAACCGAATCCTGGTTGGCCGAGTACGCTTGACCAACGTGGGTTTGTCGACTGTGCGCGGCACTTAATTGAATGTGAGCAAAACCAGACAGCTCCGCCAACCGCCGGc                                                                                                                                                                                          >  1:54252/2‑128 (MQ=255)                                                                                                                                                                                             aCCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGACTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAAcn                                                                                                                                                                                                <  1:55231/100‑2 (MQ=255)                                                                                                                                                                                                            gCAGAGTACGCTTGAGCAACGTGGGTTTGTCGACTTTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCG‑AACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTgg                         >  1:27513/1‑251 (MQ=255)                                                                                                                                                                                                                    ngCTTGAGCAACGTGGGTTTGTCGACTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCG‑AACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAg                                                            >  1:405577/2‑208 (MQ=255)                                                                                                                                                                                                                                    gTTTGTCGACTGTGCGCGGCACTTCATTGAATGTGTGCATAACCAGACAGTTCCGCAAACCACCGGCGCAAC‑GGACGTGATAGCGCAACGCATCGTTGAAAAGCTCTGGCGCGATGCGCTGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCAATGTAGACTATTCGACTCTTTCAGCGCCAGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACAGATGAATttatt  >  1:362843/1‑250 (MQ=255)                                                                                                                                                                                                                                        gTCGACTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCG‑AACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAgcgc                                                         >  1:17534/1‑191 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   AACGACGCTGGCGAAATGCTGTTTGCCGAGCACCATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAGCGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAATGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACGCTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAGACAGTTCCGCAAACCGCCGGCG‑AACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATT  >  NC_000913/1127373‑1127849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑