Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	527,162	C→T	T1301M (ACG→ATG)	rhsD →	protein RhsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	527,162	0	C	T	100.0%	43.7 / NA	16	T1301M (ACG→ATG)	rhsD	protein RhsD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/8);  total (8/8)

CTTGGGATGATGCCAGATCTGGAGCATGTACGGGGGGAGTTTGTGGTGTTCTTTCACGTATAATAGGACCAAGTAAATTTGATAGTACTGCAGATGCTGCGTTAGATGCTTTGAAAGAAACGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTA  >  NC_000913/527042‑527397                                                                                                                         |                                                                                                                                                                                                                                            cTTGGGATGATGCCAGATCTGGAGCATGTACGGGGGGAGTTTGTGGTGTTCTTTCACGTATAATAGGACCAAGTAAATTTGATAGTACTGCAGATGCTGCGTTAGATGCTTTGAAAGAAATGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATgn                                                                                                                                                                              <  1:129804/184‑2 (MQ=255)                          aTGTACGGGGGGAGTTTGTGGTGTTCTTTCACGTATAATAGGACCAAGTAAATTTGATAGTACTGCAGATGCTGCGTTAGATGCTTTGAAAGAAATGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGGAAAATATTTTGCATCGAAGGCAGAATCTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTtg                                                                                  <  1:80977/251‑1 (MQ=255)                                           gtggtgTTCTTTCACGTATAATAGGACCAAGTAAATTTGATAGTACTGCAGATGCTGCGTTAGATGCTTTGAAAGAAATGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCAc                                                                 >  1:429151/1‑251 (MQ=255)                                               ggttatatCACGTATAATAGGACCAAGTAAATTTGATAGTACTGCAGATGCTGCGTTAGATGCTTTGAAAGAAATGCAGAATAGATCTCTATGTAATTATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGTAAAATATTTTGCATCTAAGGCAGAAACTGATAATGTAAGAAAGGAGTCATATcn                                                                                                                        <  1:100444/185‑2 (MQ=255)                                                   cTTTCACGTATAATAGGACCAAGTAAATTTGATAGTACGGCAGATGCTGCGTTAGATGCTTTGAAAGAAATGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTcc                                                                                                      <  1:41288/206‑1 (MQ=255)                                                                       aaGTAAATTTGATAGTACTGCAGATGCTGCGTTAGATGCTTTGAAAGAAATGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTtg                                     <  1:266692/251‑1 (MQ=255)                                                                        aGTAAATTTGATAGTACTGCAGATGCTGCGTTAGATGCTTTGAAAGAAATGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGa                                                                                                                                                                                       >  1:11901/1‑104 (MQ=255)                                                                                  aTAGTACTGCAGATGCTGCGTTAGATGCTGTGAAAGAAATGCAGAATAGAnc                                                                                                                                                                                                                                 <  1:395196/52‑1 (MQ=255)                                                                                        cTGCAGATGCTGCGTTAGATGCTTTGAAAGAAATGCAGAATAGATCTCTATGTAATAATATGGAATACTCTGGTATTGTCAGTAAAGATACTAATGGAAAATATTTTGCATATAAGGCAGACACTGATAATTTAAGAAAAGAGTCATATCCTCTGAAAAGAAAATGTCCAACAGGTACAGATaga                                                                                      >  1:341315/1‑185 (MQ=255)                                                                                                       tAGATGCTTTGAAAGAAATGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTCAAGATACTAATGGAAAATATTTTGCAACTAAGGCAGCAACTGATAATTTAAGCAAGGAGCCATATCCTCTGACAGGAAAATGTCCCACAGGTACAGCTAGAGTTGCTGATTATCATACTCACGGTGCAGATCGTCATGGCGATTATGTTGATGACTTTTTTTc                        >  1:266228/1‑232 (MQ=255)                                                                                                          ntGCTTTGAAAGAAATGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATa             >  1:311478/2‑240 (MQ=255)                                                                                                            ncTTTGAAAGAAATGCAGAATAGATCTCTATGCAATGCTATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGACAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAACTGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTa  >  1:55545/2‑249 (MQ=255)                                                                                                            gCTTTGAAAGAAATGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAAATGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGCAAATGTCTCACAGGTACAGAt                                                                                         >  1:501382/1‑162 (MQ=255)                                                                                                             cTTTGAAAGAAATGCAGAATAGATCTNTATGTANNGANATGGAATACTCTGGTATTGNCTNNNANNNTNCTAATNGANNATATTTTGCNTCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAANTGTCCCACANNTACAGATAGAGTtgc                                                                                 >  2:109580/1‑169 (MQ=255)                                                                                                             cTTTGAAAGAAATGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGATTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAg                                                                                           <  1:197061/159‑1 (MQ=255)                                                                                                             cTTTGAAAGAAATGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTtgc                                                                                 <  1:109580/169‑1 (MQ=255)                                                                                                                         |                                                                                                                                                                                                                                            CTTGGGATGATGCCAGATCTGGAGCATGTACGGGGGGAGTTTGTGGTGTTCTTTCACGTATAATAGGACCAAGTAAATTTGATAGTACTGCAGATGCTGCGTTAGATGCTTTGAAAGAAACGCAGAATAGATCTCTATGTAATGATATGGAATACTCTGGTATTGTCTGTAAAGATACTAATGGAAAATATTTTGCATCTAAGGCAGAAACTGATAATTTAAGAAAGGAGTCATATCCTCTGAAAAGAAAATGTCCCACAGGTACAGATAGAGTTGCTGCTTATCATACTCACGGTGCAGATAGTCATGGCGATTATGTTGATGAATTTTTTTCAAGTAGCGATAAAAATCTTGTA  >  NC_000913/527042‑527397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑