Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,286,364	C→T	G172G (GGC→GGT)	narI →	nitrate reductase A subunit gamma

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,286,364	0	C	T	100.0%	30.2 / NA	14	G172G (GGC→GGT)	narI	nitrate reductase A subunit gamma
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/7);  total (7/7)

CGGAGCGGATATCCTGATCCTGTCGCTGCTCGTTATCCAGTGCGCGCTGGGCCTGTTGACCATTCCGTTCTCCGCTCAGCATATGGACGGTAGCGAGATGATGAAACTGGTTGGCTGGGCGCAGTCGGTGGTGACCTTCCACGGTGGCGCTTCTCAACACCTCGATGGTGTGGCGTTTATCTTCCGTCTGCACCTGGTGCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCT‑GGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTT  >  NC_000913/1286217‑1286593                                                                                                                                                    |                                                                                                                                                                                                                                       cGGAGCGGATATCCTGATCCTGTCGANGCCCGTNNTANAGTGCGCGCTGGGACTGTTNACNNNTNNNTNATCCGNTCNNCATATGGACNGTAGCGAGATGATGAAACTGGTTGGCTGGGCGCAGTAGGTGGTGAACTTCCACNGTGGTGGTTNNCAACACCTCGATGGTGTGGCGNTTANNTNNCNTCTNcc                                                                                                                                                                                            >  2:119035/1‑191 (MQ=255)                         gctgctCGTTATCCAGTGCGCGCTGGGCCTGTTGACCATTCCGTTCTCCGCTCAGCATATGGACGGTAGCGAGATGATGAAACTGGTTGGCTGGGCGCAGTCGGTGGTGACCTTCCACGGTGGTGCTTCTCAACACCTCGATGGTGTGGCGTTTATCTTCCGTCTGc                                                                                                                                                                                             <  1:40432/167‑1 (MQ=255)                             ctCGTTATCCAGTGCGCGCTGGGCCTGTTGACCATTCCGTTCTCCGCTCAGCATATGGACGGTAGCGAGATGATGAAACTGGTTGGCTGGGCGCAGTCGGTGGTGACCTTCCACGGTGGTGCTTCTCAACACCTCGATGGTGTGGCGTTTATCTTCCGTCTGCACCTGGTGCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACg                                                                                                     >  1:432358/1‑251 (MQ=255)                                                  ggCCTGTTGACCATTCCGTTCTCCGCTCAGCATATGGACGGTAGCGAGATGATGAAACTGGTTGGCTGGGCGCAGTCGGTGGTGACCTGCCACGGTGGTGCTTCTCAACACCTCGATGGTGTGGCGTTTATCTTCCGTCTg                                                                                                                                                                                              <  1:73592/141‑1 (MQ=255)                                                       gTTGACCATTCCGTTCTCCGCTCAGCATATGGACGGTAGCGAGATGATGAAGCTGGTTGGCTGGGCGCAGTCGGTGGTGACCTTCCACGGTGGTGCTTCTCAACACCGCGATGGTGTGGCGTTTATCTTCCGTCTGCACCTGGTGCt                                                                                                                                                                                   <  1:282585/147‑1 (MQ=255)                                                                tCCGTTTTCCGCTCAGCATATGGACGGTAGCGAGATGATGAAACTGGTTGGCTGGGCGCAGTCGGTGGTGACCGTCCAGGGTGGTGCTTCTCAACACCTTGATTGTGTGGCGTTTATCTTCCGTCTGCACCTGGTGCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACCCATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCT‑GGTGCGCGCTCGTCACTAAGCg                                                                  <  1:371999/250‑1 (MQ=255)                                                                                   atGGACGGTAGCGAGATGATGAAACTGGTTGGCTGGGCGCAGTCGGTGGTGACCTTCCACGGTGGTGCTTCTCAACACCTCGATGGTGTGGCGTTTATCTTCCGTCTGCACCTGGTGCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACAGCTTGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCT‑GGTGCGCGCTCGTCACTAAGCGAATTTTAGTTcac                                                     <  1:202409/244‑1 (MQ=255)                                                                                        cGGTAGCGAGATGATGAAACTGGTTGGCTGGGCGCAGTCGGAGGTGAACTTCCAAGGAGGTGCTTCTCAAAACATCGCTGGTGTGGCACTTTTATTCCGTCTGCACCCGGTGCTGGGGATGACGTTATTCCTGACGTTCAACTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGAAAGTACCAGCT‑GGTGCGCGCTCGTCACTCAGCGAAtttt                                                            >  1:450169/1‑232 (MQ=255)                                                                                         ggTAGCGTGATGATGAAACTGGTTGGCTGGGCGCCGTCGGTGGTGACCTTCCACGGTGGTGCTTCTCAACACCTCGATGGTGTGGCGTTTATc                                                                                                                                                                                                       >  1:428062/1‑93 (MQ=255)                                                                                                                           aGTCGGTGGTGACCTTCCACGGTGGTGCGTCTCAACACCTCGATGGTGTGGCGTTTATCTTCCGTCTGCACCTGGTGCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTTCCGGTGGAGTATCTGACACGTAAGTACCAGCT‑GGTGCGCTCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGTGTTTTTTTATGGGCACTGTGCgggttg      <  1:119035/251‑4 (MQ=255)                                                                                                                              cGGTGGTGACCTTCCACGGTGGTGCTTCTCAACACCTCGATGGTGTGGCGTTTATCTTCCGTCTGCACCTGGTGCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACAt                                                                                                                                   >  1:142675/1‑124 (MQ=255)                                                                                                                               ggtggtGACCTTCCACGGTGGTGCTTCGCAACACCTCGATGGTGTGGCGTTTATCTTCCGTCTGCACCTGGTGCTGGGGATGACGTTAGTCCTGCTGTTCCCGTTCTCGCGTCGGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCTTGGT‑CGCGCTCGTCACGAAGGGAATTTTAGTGCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGAGGTGAGtt  <  1:435738/251‑1 (MQ=255)                                                                                                                                        cTTCCACGGTGGTGCTTCTCAACACCTCGATGGTGTGGCGTTTATCTTCCGTCTGCAACTGGTGCTGGGGATGACGTTATTCCTGCTGTTCCAGTTCTCGCGTCTGATACACATCTGGAg                                                                                                                             >  1:224908/1‑120 (MQ=255)                                                                                                                                            naCGGTGGTGCTTCTCAACACCTCGATGGTGTGGCGTTTATCTTCCGTCTGCACCTGGTGCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTc                                                                                                                                             >  1:54966/2‑100 (MQ=255)                                                                                                                                                    |                                                                                                                                                                                                                                       CGGAGCGGATATCCTGATCCTGTCGCTGCTCGTTATCCAGTGCGCGCTGGGCCTGTTGACCATTCCGTTCTCCGCTCAGCATATGGACGGTAGCGAGATGATGAAACTGGTTGGCTGGGCGCAGTCGGTGGTGACCTTCCACGGTGGCGCTTCTCAACACCTCGATGGTGTGGCGTTTATCTTCCGTCTGCACCTGGTGCTGGGGATGACGTTATTCCTGCTGTTCCCGTTCTCGCGTCTGATACACATCTGGAGCGTACCGGTGGAGTATCTGACACGTAAGTACCAGCT‑GGTGCGCGCTCGTCACTAAGCGAATTTTAGTTCACATAGACCCTGCTTCGGCGGGGTTTTTTTATGGGCACGGTGCGGGGTGAGTT  >  NC_000913/1286217‑1286593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑