Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,032,624	C→T	L420L (CTG→TTG)	uacT →	urate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,032,624	0	C	T	93.3%	36.1 / NA	15	L420L (CTG→TTG)	uacT	urate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (6/8);  minor base G (0/1);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

CATGGTGCCAAAAATGGCGGTGCTGGTAGCCTCCATTCCGCAATTTGTGCTGGGCGGCGCTGGTCTAGTGATGTTCGGCATGGTACTGGCGACAGGGATTCGAATTCTGTCGCGCTGTAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTCCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATGCGCAAGCTGAAGAAA  >  NC_000913/3032401‑3032797                                                                                                                                                                                                                                |                                                                                                                                                                              cATGGTGCCAAAAATGGCGGTGCTGGTAGCCTCCATTCCCCAATTTGTGCTAGGTGCCGCTGGCCTACTGATGTTCGGCATGGAACACACGAAAGGGATTCGGCTTCTGTCGCGCTGTAACTACACCCCCAACCGTTACAACACCTATATTGTGGCGATCAGTCTCGGCGGTGGCATGACTCCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTATTGCAACCGCTGCTACATAGCggggatc                                                                                                                                                    >  1:76679/1‑246 (MQ=255)        ccAAAAATGGCGGTGCGGGTAGCCACCATTCCGCAATTTGTGCGGGGCGGCGCTGGTCTAGTGATGTTCGGCATGGTACAGGCGACAGGGATTCGAATTCGGTCGCGCTGTAACTACACCACCAACCGTTACAACCTCTATATTGTGGCTATCAGTCGCTGTGTTGGCATGACTCCGACGCTCTCTCACGATTTCTTGTCTAAGTTACCGGCCGTATTGCAACCGCTGCTACATAGCGGCATTATGCTCGc                                                                                                                                             <  1:380039/251‑1 (MQ=255)                   ggTGCTGGTAGCCTCCATTCCGCAATTTGTGCTGGGCGGCGCTGGTCTAGTGATGTTCGGCATGGTACTGGCGACAGGGATTCGAATTCTGTCGCGCTGTAACTACACCACCAACCGTTACAACCTCTATATTGGGGCGATCAGTCTCGGCGTTGGCATGACTCCGACGCTCTCTCACTATTTCTTTTCTAAGTTACCGGCCGTATTGCAACCGCTGCTACATAGCGGc                                                                                                                                                        <  1:197564/229‑1 (MQ=255)                                                 gCTGGGCGGCGCTGGTCTAGTGATGTTCGGCATGGTACTGGCGACAGGGATTCGAATTCTGTCGCGCTGTAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTCCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTATTGCAACCGCTGCTACATAGCgn                                                                                                                                                         <  1:310770/198‑2 (MQ=255)                                                                                   ntACTGGCGACAGGGATTCGAATTCTGTCGCGCTGTAACAACACCACCACCCGGTACAACCTCTAAATTGTGGCGCTCAGTCTCCGCGTTGGCATGACTCCGACGCTCTCTCCCGCTTTCTTTTCTAAGTTACCGGCCGTATTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTCGCGCCGTTGTGc                                                                                                                         >  1:53253/2‑196 (MQ=255)                                                                                                                     gTAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTCCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTATTGCAACCg                                                                                                                                                                       <  1:217031/116‑1 (MQ=255)                                                                                                                                 ccaACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTACGACGCTCTCTCACGATTTCTTTTATAAGTTACCGGCCGTATTGCACCCGCTGCTACATAGCGACATTATGCTCGCAACCCTTAGCGCAGTTGTGCTGAACGTCTTCTTTCATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATACAGATTTAAAAGTCAGAACAGTACGTATGTGGCTTCTg                    >  1:226337/1‑251 (MQ=255)                                                                                                                                     ccGTTACAACCTCTATCTTGTGGCGATCAGTCTCGGCGTTGGCATGACTCCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTATTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTgga                                                                                                                     >  1:262776/1‑148 (MQ=255)                                                                                                                                     ccGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTCCGACGCTCTCTCACGATTTCTTTTCTAATTTACCTGCCGTATTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCGTTAATGTCTATCAGCATCATTCGGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATgc                <  1:458564/251‑1 (MQ=255)                                                                                                                                                               tCAGTCTCGGCGTTGGCATGACTCCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTATTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTtgtn                                                                                                                          <  1:147812/119‑2 (MQ=255)                                                                                                                                                                    ctcGGCGTTGGCATGACTCCGACGCTNTCTCACNNTTNCTTTTCTAAGTTACCGGCCNTANNNCNNNCNCTGCTNCANNGCGGCATTANGCTCGCAACCCTTAGCGCCGTTGTGCTGAACg                                                                                                                   >  2:118519/1‑121 (MQ=255)                                                                                                                                                                    ctcGGCGTTGGCATGACTCCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTATTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACg                                                                                                                   <  1:118519/121‑1 (MQ=255)                                                                                                                                                                                                  cACGATTTCTTTTCTAAGTTACCGTCCGTATTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGTACGTCTTCTTTAATGGCTATCAGCATCATGCTTACCTGGTGAAGGAATCCGTCTCTGATAACGCTTTAAAAGTCAGGACACGACGTATGTGGCTTCTGATGCGCAAGCTGAAGaaa  >  1:11498/1‑204 (MQ=255)                                                                                                                                                                                                      aTTTCTTTTCTAAGTTACCGGCCGTATTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTCAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGc                         <  1:237072/177‑1 (MQ=255)                                                                                                                                                                                                                 aaggtACCGGCCGTAGGGCAACCGCTGCTACATAGCGGCATTAGGCTCGCAACCCGTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGc                                                                                       <  1:167799/100‑1 (MQ=255)                                                                                                                                                                                                                       ccGGCCGTATTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTNAAANNNNNggnn                                       >  2:307702/1‑144 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                              CATGGTGCCAAAAATGGCGGTGCTGGTAGCCTCCATTCCGCAATTTGTGCTGGGCGGCGCTGGTCTAGTGATGTTCGGCATGGTACTGGCGACAGGGATTCGAATTCTGTCGCGCTGTAACTACACCACCAACCGTTACAACCTCTATATTGTGGCGATCAGTCTCGGCGTTGGCATGACTCCGACGCTCTCTCACGATTTCTTTTCTAAGTTACCGGCCGTACTGCAACCGCTGCTACATAGCGGCATTATGCTCGCAACCCTTAGCGCCGTTGTGCTGAACGTCTTCTTTAATGGCTATCAGCATCATGCTGACCTGGTGAAGGAATCCGTCTCTGATAAAGATTTAAAAGTCAGGACAGTACGTATGTGGCTTCTGATGCGCAAGCTGAAGAAA  >  NC_000913/3032401‑3032797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑