Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,722,605	G→A	E885K (GAA→AAA)	patZ →	peptidyl‑lysine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,722,605	0	G	A	100.0%	78.7 / NA	27	E885K (GAA→AAA)	patZ	peptidyl‑lysine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/11);  total (16/11)

CATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCTTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCCCGC  >  NC_000913/2722367‑2722827                                                                                                                                                                                                                                               |                                                                                                                                                                                                                               cATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTCATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGACTGGTATTACGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAATTCTTGCCCAGCGCGAGAACTCATGAGTa                                                                                                                                                                                                                     >  1:87652/1‑250 (MQ=255)                                       ncAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGgtgt                                                                                                                                                                              >  1:302444/2‑251 (MQ=255)                                                                       gAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGca                                                                                                                                              <  1:31746/251‑1 (MQ=255)                                                                                           gcgAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCTAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGTGACTGGTGTTATTATTGCCCTCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAAc                                                                                                                          <  1:231196/251‑1 (MQ=255)                                                                                                aTCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGtt                                                                                                                                                                                                     <  1:206187/171‑1 (MQ=255)                                                                                                               gTCTGAATGGTATTACGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGCTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACtgtg                                                                                                       >  1:455420/1‑250 (MQ=255)                                                                                                                              cGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGCAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGNAAANNNNNAcnnnnnnnnnn                                                                                                                                                                                        >  2:308340/1‑144 (MQ=255)                                                                                                                              cGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATgtn                                                                                                                                                          <  1:308340/184‑2 (MQ=255)                                                                                                                                  gCCAAACAATCGGGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACTTTGATATCCAGCTCGAAGAGAGGAGCGTTGGGCTTACGCTAAATCTTGCCGAGCGCGAGAAATCATGAGTAACGTACTGGAAATGTTGACCACTTAATCGTGAGTGATGTTATTAGTGCCCGCCTATGGCGTGTCCATTTGACTGAGGACTTTTCAATGAACAGAGAAGAAATGCGTTGTGATGTTGTCAAAATTTAAgag                                                                                   <  1:460732/251‑3 (MQ=255)                                                                                                                                    cAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCGGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGgtg                                                                                                                                                                               >  1:262168/1‑157 (MQ=255)                                                                                                                                              nnnnnnggnnnNNCTANCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGtta                                                                                                                                                                            <  2:302444/144‑1 (MQ=255)                                                                                                                                                     gtggCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCAAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTTTTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAacac                                                                >  1:434067/1‑251 (MQ=255)                                                                                                                                                       ggCGCTAGCCCGCAAGCTCGGGATTAACGTTGATATCCAGATCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATc                                                                        >  1:181008/1‑241 (MQ=255)                                                                                                                                                        ncgcTAGCCCGCAAGCTAGGGTTTACCGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTattatt                                                                                                                                                                       >  1:404403/2‑145 (MQ=255)                                                                                                                                                               cccGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGc                                                                                                                                                                     <  1:400224/140‑1 (MQ=255)                                                                                                                                                                 cGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCTTGCCCAACt                                                     >  1:282542/1‑250 (MQ=255)                                                                                                                                                                                      gatctCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGAGCGAGAAATCATGAGTACGGTACTGGAAATGTTGACCACTTAATCGGGACTGgtg                                                                                                                                                                               >  1:297133/5‑107 (MQ=255)                                                                                                                                                                                          tCCAGCTCGAAGAGGGGATCGTGGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAAGGTTTACCACTTAATCGGGACTGGTGTGATTATTGCCAGCTGATGGCGTCTGCATTGCACAGAGGAc                                                                                                                                      <  1:216496/144‑1 (MQ=255)                                                                                                                                                                                                 cGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCTTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCg                     >  1:168158/1‑250 (MQ=255)                                                                                                                                                                                                   aaGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCTTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGAtt                  >  1:24475/1‑251 (MQ=255)                                                                                                                                                                                                          gATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATCGCCCTCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACTGTGCTGTTGTCAAAATTTAAg                                                                                     >  1:2722/1‑177 (MQ=255)                                                                                                                                                                                                             cGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCTTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACg         <  1:83393/250‑1 (MQ=255)                                                                                                                                                                                                                  ggCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACANAGANNNNNTgnn                                                                                                            >  2:297521/1‑144 (MQ=255)                                                                                                                                                                                                                  ggCTTACGCTAAATCTTGCCCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTcn                                                                                              <  1:297521/160‑2 (MQ=255)                                                                                                                                                                                                                   gCTTACGCTAACTCTTGACCAGCGCGAGAAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCTTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCccgc  >  1:399390/1‑251 (MQ=255)                                                                                                                                                                                                                   gCTTACGCTAAATCTTGCCCAGCGCGAGAACTCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGc                                                                                                                                                                 >  1:397410/1‑92 (MQ=255)                                                                                                                                                                                                                    cTTACGCTAAATCTTGCCCAGCGCGAGAAATAATGAGTAAGGGACTGGAAATGTTGACCACGTAATCGGGACTGGTGTTATTATTGcctgc                                                                                                                                                                 <  1:168342/91‑4 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                               CATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAATCGTGGCATGGTGGCGCTAGCCCGCAAGCTCGGGTTTAACGTTGATATCCAGCTCGAAGAGGGGATCGTTGGGCTTACGCTAAATCTTGCCCAGCGCGAGGAATCATGAGTAAGGTACTGGAAATGTTGACCACTTAATCGGGACTGGTGTTATTATTGCCCGCTTATGTCGTCTGCATTGCACAGAGGACCCTTCAATGAACAGAGAAGAAATGCACTGTGATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCTTGCCCAACTACCCAAGATTTCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCCCGC  >  NC_000913/2722367‑2722827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑