Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,336,411	2 bp→AT	coding (83‑84/1260 nt)	murA ←	UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,336,411	0	G	A	95.7%	60.6 / ‑4.1	23	I28N (ATC→AAT)	murA	UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (11/11);  total (11/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01
*	NC_000913	3,336,412	0	A	T	95.2%	54.9 / NA	22	I28N (ATC→AAT)	murA	UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base T (11/9);  minor base G (0/1);  total (11/11)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGAC  >  NC_000913/3336204‑3336652                                                                                                                                                                                                                ||                                                                                                                                                                                                                                                 cAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGc                                                                                                                                                                                                        <  1:111547/251‑1 (MQ=255)                  cACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCCATATGCCCAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTCCCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGa                                                                                                                                                                                                                      >  1:460322/1‑220 (MQ=255)                  cACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTggn                                                                                                                                                                                       <  1:405916/251‑2 (MQ=255)                                  cAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACAATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAAtt                                                                                                                                                                       >  1:80638/1‑251 (MQ=255)                                      tCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATTCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGGTTCGTGACGTTCTGGAGCTCTACCGGTTCTGCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTGTTAGCGCCGGAAATGGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGa                                                                                                                                                                                 <  1:334928/237‑1 (MQ=255)                                        gTAAGGAGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATcc                                                                                                                                                                  >  1:174622/1‑250 (MQ=255)                                                cgcAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCTGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGAc                                                                                                                                                                                                             >  1:414208/1‑199 (MQ=255)                                                             aaCGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCCCCCCGATGGCTTAGCAGCTTCATTGATGTATAGCCGTCTTTCAGTTTCGGGACGTTATGGATCTCTACCGGTTCTTTCGCCCATAGTGCGGCAAAAAGATTAGGCaga                                                                                                                                                                                                                                          >  1:296797/1‑157 (MQ=255)                                                                   gcgGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTc                                                                                                                                                                                                          <  1:156517/183‑1 (MQ=255)                                                                               atGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTggn                                                                                                                                                                                       <  1:89960/190‑2 (MQ=255)                                                                                         ccATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCg                                                                                                                                                                                                                        <  1:189984/147‑1 (MQ=255)                                                                                                                           gcagcTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAACAAGATTAGGCAGAGCAGCATTTTTAgcg                                                                                                                                                                                                                           >  1:265562/1‑110 (MQ=255)                                                                                                                             agtttCATTGATGTATCGAAGTCTTTCAGTTTCGGGACGTTCGGGATCTCTACCGGTTATTCCGCCAGTAGTGCGGCAAAAAGGAGAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGGTACTTCGCCCTGGATCGTCGTTGGACACTTAACACGAAATTTATCCATTTAGTCTGTACCCAGTTAACATTGCATATCCGCTACCGGCGAATCTCCCATAGCTCACAAGCCGTTCAGTTTGCGATcgcgctt                                                                            <  1:366002/248‑3 (MQ=255)                                                                                                                              tcTTCCTTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGCAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTa                                                                                                                                                                                                                              >  1:389985/2‑104 (MQ=255)                                                                                                                                              naCGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTCGGCCGAGCAGCATTTT‑AGCGCCGGAAATTGTGACTTCGCCCTGGCGCTTAGTTGGCCCCTGAACAAGAAATTTATCCATTTAGTTTGTTCTCCGTTAACAATTAATATCCGCTAc                                                                                                                            >  1:102683/2‑185 (MQ=255)                                                                                                                                                   cTTTCAGTTTAGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTATTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATcg                                                                                 <  1:412771/224‑1 (MQ=255)                                                                                                                                                        aGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGAGTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACGTCGCCCTGGAGCTTCGTGGGCCCCTGAACACGAAATTTATCCAGTTAGTTTGTTCTCAGTTAACAAGTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTcc                                                                     <  1:282210/231‑1 (MQ=255)                                                                                                                                                          tttCGGGACGTTCTGGCTCTCTACCGGTTCGTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATGTTTAGCGCCGTAAATTGTGACTGCGCTCTGGAGCTTCGTTTGCCCCGGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTa                                                                                                                                              <  1:294957/156‑1 (MQ=255)                                                                                                                                                                         gATCTCTCNCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGANTNGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCNTCGTTGGCCCNTGANNACGANANTNATCCATTTAGTGTTTTCTCAGTTAACAATNCATATCCGCTACCGGCGAATCGCNNTTNNNTCNNAAGcc                                                                                                 <  2:369558/186‑1 (MQ=255)                                                                                                                                                                         gATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGAAGCATTTTTAGCGCCGGAAATTGTGACTTCACCCTGGAGATTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGcc                                                                                                 >  1:369558/1‑186 (MQ=255)                                                                                                                                                                                          ccGCCAGTAGTGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCATGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCCACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCg                                                                                     >  1:325583/1‑181 (MQ=255)                                                                                                                                                                                               agtagtGCGGCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATAGCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCTACACAGCATGAATGCGGTTATCCGCAATATATTCCAt          <  1:274378/251‑1 (MQ=255)                                                                                                                                                                                                       ggCAAAAAGATTAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCATGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGAc  >  1:75992/1‑251 (MQ=255)                                                                                                                                                                                                                ||                                                                                                                                                                                                                                                 CAGCGCCCAGATAGAAGCACGCATGGTTTTAACCAGATCGTAAGGTGCGCAGAATACATTAACGTCGCGGGCATCAATATGCACAGAACCATTACGTTCTACTTTCGCACCCAGCTGGCTTAGCAGCTTCATTGATGTATCGACGTCTTTCAGTTTCGGGACGTTCTGGATCTCTACCGGTTCTTCCGCCAGTAGTGCGGCAAAAAGGATAGGCAGAGCAGCATTTTTAGCGCCGGAAATTGTGACTTCGCCCTGGAGCTTCGTTGGCCCCTGAACACGAAATTTATCCATTTAGTTTGTTCTCAGTTAACAATTCATATCCGCTACCGGCGAATCGCCCATAGCTCAAAAGCCGTTCAGTTTGCGATCGCGCGCCCACTCCGCAGGGGTATACGCTTTGATCGACACAGCATGAATGCGGTTATCCGCAATATATTCCATCAGCGGAC  >  NC_000913/3336204‑3336652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑