| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 478,694 | G→A | intergenic (‑77/+87) | pdeB ← / ← ylaC | c‑di‑GMP phosphodiesterase PdeB/putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 478,694 | 0 | G | A | 96.9% | 85.8 / ‑5.8 | 32 | intergenic (‑77/+87) | pdeB/ylaC | c‑di‑GMP phosphodiesterase PdeB/putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (15/16); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGGAGAGTTCAAGATGGCTTCAACC > NC_000913/478484‑478913 | gaATAGGTATCCAGCTCTTCAATAAACGATGCTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTg > 1:156489/1‑251 (MQ=255) ctgctgATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGCGGTAAAGCTGGTATTTc > 1:339238/1‑185 (MQ=255) gctctgatcTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTGCATGAATTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTTATTCGTCGcgagcgag < 1:179965/242‑1 (MQ=255) gAAAGAATCAGTGCTCCCGAAAGAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCCTGAAGTGAGAGGTGGAAACGATCATTGTCATTTGACCAGGCGCaga < 1:17248/186‑1 (MQ=255) gAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCAGATCCTGATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTAGTTCATGAAGTTAGGGGTGGAAACTATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTg < 1:257235/198‑1 (MQ=255) tCAGTACTCCCGAAATAAGGCCCACCAGATGTCGTGTTCTCAACAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTTGCTGGAGATAAGGAGGTAAATCTGGTATTTCATGAAGTGAGGGGTGGAAACGATCATTGTCATTTGACCAGTCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGcgagcgag < 1:347621/233‑1 (MQ=255) tCCCGAAATAAGGCCGACCAGAGGTCGTGTTCTCTCCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTTGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTgg < 1:362145/141‑1 (MQ=255) tCCCGAAANANNNCNNACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGNAGGGAAAGTATATCTNATNGTGGNAAANGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTgg > 2:362145/1‑141 (MQ=255) ccaaaaTAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTc > 1:282063/4‑251 (MQ=255) ccGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATCTCCTTATTACCCCCGTGTAGGGACTGTATCTCTCATCGTGGAACACGCATGGCTGGAGCCAAGCCGGAAACGCTGGAATTTATTGCAGTGATGGGTGGACACGATCATTGTCATTTGACCAGGCTCAGAGCTTTAAGcc > 1:255575/1‑180 (MQ=255) ccGAAATAAGGCCGACCAGATGTCGTGTGCTCGCCAGCATATCCTTATTAACACAGTGTATGGAAAGTATATCTGATTGTTGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGAt < 1:280174/133‑1 (MQ=255) cGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTAc > 1:145700/1‑176 (MQ=255) aCCAGATGTCGTGTTCTCACCAGCATATCCGTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGCAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGCGCGAGGGTaaa > 1:24059/1‑216 (MQ=255) aGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAgg < 1:409783/147‑1 (MQ=255) cGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGCAAAAGGCTGGCTGGAGATAAGGAGGTCAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGCTTCGGCGCGAGCGAGGGTaaaaa > 1:443946/1‑209 (MQ=255) gtTCTCACCAGCATATCCTTATTAACACCGTGTAGGGCAAGGATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCCTTGTCATTTGACCCGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATAGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGc > 1:12490/1‑250 (MQ=255) gtTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATCAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGc > 1:327243/1‑250 (MQ=255) agctcACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAg < 1:20835/249‑1 (MQ=255) tatCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACg < 1:447057/225‑1 (MQ=255) aaCACAGTGTAGGGAAAGTATATCTGCTTGTGGAAAAAGGCTGGCTGGAGATAAGGCGGTAAAGCTGGTATTTCATGACGTGATGGGTGGAACCGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGACCATCcgcg > 1:78935/1‑251 (MQ=255) aaCACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTg < 1:437307/89‑1 (MQ=255) aaCACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACCTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACg > 1:497586/1‑211 (MQ=255) acacAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCgn < 1:50202/164‑2 (MQ=255) acacAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCAc > 1:388254/1‑203 (MQ=255) tagcTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGAtn < 1:99573/62‑2 (MQ=255) gATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGCTGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgcg > 1:188565/1‑142 (MQ=255) gAAAAAGGCTGGCTGGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGCGATGGGTGGACAAGAACATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATACGGCGCGAGCGTGGGTAATAATATCGTAACAAGACAGTTCACCTTTACGGAGGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGGAGAGTTCAAGATGGCTTCAAcc > 1:487513/1‑249 (MQ=255) gctggntgNANATNNGNAGGTNAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCAntnt > 2:251331/1‑64 (MQ=255) gctggctgGAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGTGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAc < 1:251331/103‑1 (MQ=255) gctggctgGAGATAAGGAGGGAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATg < 1:379658/99‑1 (MQ=255) gctggAGATAAGGAGGTAAAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTGTTGCAGTTGTTcn < 1:122687/193‑2 (MQ=255) tAAGGAGGTAAAGCTGGGATTTCATGAAGTGATGGGTGGAGACGATCATGGTCAGTTGACCAGGCGCAGAGCTTGACGCCTGGTATGTAACCCTAACTGAAGATGTTGAGTCGGCGCGAGCGAGGGTAAAAATATCg < 1:65186/137‑1 (MQ=255) | GAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCTCACCAGCATATCCTTATTAACACAGTGTAGGGAAAGTATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGATCATTTTTTGCAGTTGTTCCTTATGAACATCCGCGACACGCGGAGAGTTCAAGATGGCTTCAACC > NC_000913/478484‑478913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |