Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,113,805	G→A	*76* (TGA→TAA)	yceK →	DUF1375 domain‑containing lipoprotein YceK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,113,805	0	G	A	100.0%	39.3 / NA	14	*76* (TGA→TAA)	yceK	DUF1375 domain‑containing lipoprotein YceK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/10);  total (4/10)

TGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTGATTAACGTTCATCCCACTCATCAGCTGCTGCGCGTCCTTCCTCGGTATCCAGCGGTGGCTCAAGCTGAAATTCCCCCTCGTCCCATTCATGTAATGTATTCTCTTCCTGCCACTCCTGGCGTATCTCTATCTCATCATAGTCGCCATC  >  NC_000913/1113590‑1113953                                                                                                                                                                                                                        |                                                                                                                                                     tgtgGTGAGCATCATGGTGTCCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTAATTAACGTGCATCCCACTCATCAGCTGCTGcgcg                                                                                                                    <  1:155465/250‑1 (MQ=255)       gAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTAATTAACGTTCATCCCACTCATCAGCTGCTGCGCGtccttcc                                                                                                             <  1:116769/251‑1 (MQ=255)                                                                ggtgcaggggcaTGGCAACCAATTTTATCCCGGTGTGGCATGGGATGTGGGTGTCTCCGCCTGGCGTTATGTCCAGACCCTTGATCTGGTATTATCTCTGGTTTTTGATACTTTACTGCTGCCGCTCGAAATTCCTCATGGCCCGTATGGGTAATTAAGGTTCATTCCCCTCCTCAGCTGGTTCGCGTCCTTCCTCGGTCTCCAGCGGTGGCTCACGCTGAAAtt                                                                              >  1:211505/4‑225 (MQ=255)                                                                           aTGGCAACCAATATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTAATTAACGTTCATCCCACTCATCAGCTGCTGCGCGTCCTTCCTCGGTATCCAGCGGTGGCTCAAGCTGAAATTcc                                                                            <  1:374325/216‑1 (MQ=255)                                                                                    aaTATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTANTTANNNNNc                                                                                                                                            >  2:306677/1‑143 (MQ=255)                                                                                    aaTATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTAATTAACGTTCATCCCACTCATCAgctgn                                                                                                                          <  1:306677/161‑2 (MQ=255)                                                                                                    gCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTAATTAACGTTCATCCCANTCANNNnntgnn                                                                                                                         >  2:308690/1‑144 (MQ=255)                                                                                                    gCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTAATTAACGTTCATCCCACTCATCAGCTGCTgcgcn                                                                                                                    <  1:308690/151‑2 (MQ=255)                                                                                                                              ggCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCAGATCGACATTCATCATGGCCCGTTTGAGTAATTAACGTTCATCCCACTCATCAGCTGCTGCGCGtcc                                                                                                                 >  1:501186/1‑128 (MQ=255)                                                                                                                               gCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTAATTAACGTTCATCCCACTCATCAGCTGCTGCGCGTCCTTCCTCGGTATCCAGCGGt                                                                                              <  1:80716/146‑1 (MQ=255)                                                                                                                                                                    gTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTAAGTAACGTTCATCCCACTCATCAGCTGCTGCGCGTCCTTCCTCGGTATCCAGCGGtn                                                                                             <  1:94280/110‑2 (MQ=255)                                                                                                                                                                            tACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTAATTAACGTTCATCCCACTCATCAGCTGCTGCGCGTCCTTCCTCGGTATCCAGCGGTGGCTCAAGCTGAAATTCCCCCTCGTCCCATTCATGTAATGTATtct                                                <  1:263861/147‑1 (MQ=255)                                                                                                                                                                              cTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTAATTAACGTTCATCCCACTCATCAGCTGCTGCGCGTCCTTCCTCGGTATCCAGCGGTGGCTCAAGCTGAAATTCCCCCTCGTCCCATTCATGTAATGTATTCTCTTCCTGCCACTCCTGGCGTATCTCTATCTCATCATAGTCGCCATc  <  1:174366/191‑1 (MQ=255)                                                                                                                                                                                                          nggCCCGNNTGAGTAATTAACGTTCATCCCACTCATCAGCTGCTGCGCGTCCTTCCTCGGTATCCAGCGGTGGCTCAAGCTGAAATTCCCCCTCGTc                                                                    <  2:15222/96‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                     TGTGGTGAGCATCATGGTGACCTTGCTGAGTGGCTGTGGCAGCATTATTAGTCGCACTATACCGGGGCAGGGGCATGGCAACCAATATTATCCCGGTGTGCAATGGGATGTGCGTGACTCCGCCTGGCGTTATGTCACGATCCTTGATCTGCCATTCTCTCTGGTTTTTGATACTTTACTGCTGCCGATCGACATTCATCATGGCCCGTATGAGTGATTAACGTTCATCCCACTCATCAGCTGCTGCGCGTCCTTCCTCGGTATCCAGCGGTGGCTCAAGCTGAAATTCCCCCTCGTCCCATTCATGTAATGTATTCTCTTCCTGCCACTCCTGGCGTATCTCTATCTCATCATAGTCGCCATC  >  NC_000913/1113590‑1113953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑