Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	26,614	G→A	G113D (GGC→GAC)	ispH →	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	26,614	0	G	A	100.0%	31.1 / NA	15	G113D (GGC→GAC)	ispH	4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/4);  total (11/4)

TGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTC  >  NC_000913/26422‑26843                                                                                                                                                                                                 |                                                                                                                                                                                                                                      tGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGACGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATggg                                                                                                                                                                              <  1:482230/250‑1 (MQ=255)                                           tACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGACGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGACGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCg                                                                                                                                   >  1:467340/1‑251 (MQ=255)                                           tACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGAGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGACGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGc                                                                                                                                                                             >  1:31061/1‑209 (MQ=255)                                                  cGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTCACGAAGCCAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCACAGGGCATATGGAAGTCGCCCGCGGCCGTCGCCGTGACGAAGAATCTATTCTCATTGGTCACGCCGGGCACCCGGGAGTGGAAGGGACAATGGGACCGGACAGTAACCCGGAAGGGGGAATGTAACTGGTCGAATCGCCGGa                                                                                                                              >  1:446367/1‑249 (MQ=255)                                                  cGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCCCCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGACGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGCAGTGGAAGGGACAATGGGCcagtac                                                                                                                                                                       >  1:80840/1‑208 (MQ=255)                                                           ccTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGAGTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGACGAAGAATCTATGCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGTGACAATGGGCcagtac                                                                                                                                                                       <  1:67048/199‑1 (MQ=255)                                                                                             gTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATCCAACCTGTCCGCTGGTGTCCAAAGTGCATCTGGAAGTCGCCCGCGCCAGTCGCCGTGACGAAGAATCTCTTCTCATCGGTCACGCCGGGCACCCGGACGGGGAAGGGACAATGGGCca                                                                                                                                                                           >  1:86806/1‑161 (MQ=255)                                                                                                     cGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGACGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGcc                                                                                                                                                                            >  1:474703/1‑152 (MQ=255)                                                                                                     cGAAGCAAAAAGTAGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGACGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAg                                                                                                                                                                                              >  1:400772/1‑134 (MQ=255)                                                                                                        aGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCAGCTGGTGACCAAAGCGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGACGAAGAATCTATTCTCATCGGTAACGCCGCGCACCCGGAAGTGGAAGGGAAAATAGGCCCGTACAGTAACCCGGAAGGGGGAGTGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGAccc                                                                         >  1:287588/1‑248 (MQ=255)                                                                                                                                                                  cATATNGAAGTNGNNCGCGCCAGTCGCCGTGACGNAGAATCTATtc                                                                                                                                                                                                                         >  2:198398/1‑46 (MQ=255)                                                                                                                                                                  cATATNGAAGTNGNNCGCGCCAGTCACCGTGACGNAGAATCTATTCTCATNGGTCNCGCCGNGCNcn                                                                                                                                                                                                    >  2:209190/1‑66 (MQ=255)                                                                                                                                                                  cATATGGAAGGCGCCCGCGCCAGTCGCCGTGACGAATAATCTATTCTCAGCGGTCACGCCTGGCACCCGGAAGTGGAATGGACAATGGGCCAGTACAGGAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACTATGTGGGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGAc                                                                     <  1:209190/194‑1 (MQ=255)                                                                                                                                                                   atatGGAAGTCGCCCGCGCCAGTCGCCGTGACGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCAGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACCCGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCg           >  1:291062/1‑251 (MQ=255)                                                                                                                                                                             cGCCCGCGCCAGTCGCCGTGACGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGAAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTc  <  1:496198/250‑1 (MQ=255)                                                                                                                                                                                                 |                                                                                                                                                                                                                                      TGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCACCCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTC  >  NC_000913/26422‑26843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑