| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,113,851 | G→A | H208Y (CAT→TAT) | fpr ← | flavodoxin/ferredoxin‑NADP(+) reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,113,851 | 0 | G | A | 95.0% | 49.3 / NA | 20 | H208Y (CAT→TAT) | fpr | flavodoxin/ferredoxin‑NADP(+) reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (10/9); minor base C (1/0); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
GGTCATCATCACTCATTTATCAACCGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATGGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCACCAGGACCAGATTTTTGAAGCGATCTAAATCTTTACC > NC_000913/4113620‑4114091 | ggTCATCATCACTCATTTATCAACCGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTA‑TGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATc < 1:280942/250‑1 (MQ=255) gATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATAGATAAGTAACCACTTCCCAGTAATGCTCAGCTGTCATCTGGCCCGGTCGGCGCCGTAAATGTTTAGTCATCTGACGGGTCTCTTTCAGCAACTGTTGTGTCTCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTcccc > 1:335899/1‑251 (MQ=255) gATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCttt > 1:35891/1‑177 (MQ=255) cAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGttt > 1:22385/1‑251 (MQ=255) cccGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACt > 1:160308/1‑203 (MQ=255) ncGACGTNNATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGtt < 2:40065/96‑1 (MQ=255) tAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTc < 1:82237/183‑1 (MQ=255) aaaTGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTCTGTATCGCGCACCATCTGAGGATCGCAGCACAGCATCACATAGGAGGTTTATTTATTCATCCGCAGGCCAATCGTGCTTTACAGTTCCCCACTTTCCATTAATGCCGGTATCCGTCCGGTGAGCGACCACGCTGCCGTTTCCCGAc > 1:283590/1‑187 (MQ=255) aaaTGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGATGTGTATCGCGAACCATCTGTGGCTTGACGCACAGCATCACATCGCTGGTTTCTTTATTCATAGGCAGGCCAATCGTGCTGTCCACGTCCCCACTTTCAATTAATGCCGGTATCCGTTCGGTGAGCCCCCCCACTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCtt > 1:224276/1‑217 (MQ=255) cAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTAGCCGGCCGGTGAGCGACACCGCTGCCGTGTCCCGACTGACCACCGTCTGAATGCGCAGGTTGCCGTCGGAGCGGTTTGCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTgcg < 1:370553/250‑1 (MQ=255) aaCTGTTGTGTATCGCTCACCATCTGTGGATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGGGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGAcc < 1:334261/136‑1 (MQ=255) cgatccgtATCGCGCACCATCTGTGGATGGCCGCACAGCAGCACATAGCTGGGTTCTTTATTCATCGGCAGTCCAATCGTGCTCTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCTACCCCTCTGCTGTTGCCCGACTTACCACCGTCTTAATGCGCAGTTTTCCTTCGGAGCCTGTTTCCAGTTCCCGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCAc < 1:364606/245‑1 (MQ=255) gttgtGTAGCGCTCACCATCTGTGGATTGCCGCACATCATCACATAGCTGGTTTCTTTATTCATCTGCAGGCCAATCTTGCTTTCCAGTTCCCCACTGTCAAATAATGCCGGTATCCTGCCGGTGATCGACCCCGCGTCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTcc < 1:337070/178‑1 (MQ=255) gcgcACCATCTGTGGATTGCCGCACAGCATCACCTAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCtt > 1:159751/1‑73 (MQ=255) nggATTGCCGCACAGCATCACATAGCTGGTTTCTTTATTTATCGGCAGGCCACCCGGGCTTTCCAGTTCCCCAATTTAACTTAATGCCGGTATCCATCCGGTGGGCGACCCCCCTGCCGTTTCCCGACTGACCCCAGTCTCAATGCGCCGTTTTCCTTCGTAGAGATTTTCCAGTTCATGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTg > 1:59418/2‑218 (MQ=255) ccGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCGCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCTACCCCGCTGCCGTTGCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTGCGTAGCGTTTTTc < 1:322755/164‑1 (MQ=255) ccGCACAGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCCGTTTTCCTTCGTAGCGTTTTTCCAGtt > 1:323465/1‑169 (MQ=255) cacaGCATCACATAGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTACCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCACCAGGACCAGATTTTTGAAGCGATCTAAATCttt > 1:433941/1‑251 (MQ=255) aGCATCACATAGCTGTTTTCTTTATTCATCGGCAGGCCAATCGTGCTGTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGGTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTGTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACTTGCGGCTTGCACCAGGCCCAGATTTTTGAAGCGATCTAAACCTTTatc < 1:297053/251‑3 (MQ=255) tCACATAGCTGGTTTCTTTATTCATCGGCCGTCCAATCGCGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCAGTCCGGTGAGCCACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGACTGCGCAGTTTTCCTTCGTAGCGTTTTTc > 1:220658/1‑154 (MQ=255) | GGTCATCATCACTCATTTATCAACCGTTTGATGCCGGCATATCGCGGGATGATCAATAAGATGAGTGCGGATTTGCTCATCGTGCCGTTTATCGATAAGTAACCGCTTACCAGTAATGCTCCGCTGTCATATGGCCCGGTCGGCGACGTAAATGTTTCGTCATCTGCCGGGTCTCTTTCAGCAACTGTTGTGTATCGCGCACCATCTGTGGATTGCCGCACAGCATCACATGGCTGGTTTCTTTATTCATCGGCAGGCCAATCGTGCTTTCCAGTTCCCCACTTTCAATTAATGCCGGTATCCGTCCGGTGAGCGACCCCGCTGCCGTTTCCCGACTGACCACCGTCTGAATGCGCAGTTTTCCTTCGTAGCGTTTTTCCAGTTCCTGCATCAGTGGCAAATAGCTTAAGTCGGCGGCATAACGTGCGGCGTGCACCAGGACCAGATTTTTGAAGCGATCTAAATCTTTACC > NC_000913/4113620‑4114091 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |