Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,725,233	C→T	G172R (GGA→AGA)	kgtP ←	alpha‑ketoglutarate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,725,233	0	C	T	100.0%	36.1 / NA	16	G172R (GGA→AGA)	kgtP	alpha‑ketoglutarate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/10);  total (6/10)

AATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCG  >  NC_000913/2725013‑2725417                                                                                                                                                                                                                             |                                                                                                                                                                                         aaTGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAatgatgn                                                                                                                                                            <  1:128711/251‑2 (MQ=255)                                                                                             tAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGc                                                                                                                                 <  1:152801/186‑1 (MQ=255)                                                                                                                gCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAatgatg                                                                                                                                                             >  1:237105/1‑139 (MQ=255)                                                                                                                   aaCACAGCTCCTAACGCGAAAGGAATACGCCAGCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGccn                                           <  1:313772/250‑2 (MQ=255)                                                                                                                      acaGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCATTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCttt                                                                                                                                                <  1:415010/146‑1 (MQ=255)                                                                                                                         gCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACACCCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAatgat                                                                                                                                                              >  1:86364/1‑129 (MQ=255)                                                                                                                                    gAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGACTTCCATGGTGTGTTTGAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCGTTGCTCCCTTCAACTGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTcc                                                                                          <  1:506948/186‑1 (MQ=255)                                                                                                                                             aggCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGCGTGTTGTAAACCCACGAAAACACGTAGGGCTCGCCATCATCTGCCGATCAACGTCACCTACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCACCGCCAACTTCACTCCTATAGGTGGCGCTGGTGCCATATTCTCCGCCACCAGATAATCCCTGAAATAAACGAGCGAGAAGc                                                     >  1:462078/3‑214 (MQ=255)                                                                                                                                                 cATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGNNACATACNNa                                                                                                                                                                    >  2:11641/1‑99 (MQ=255)                                                                                                                                                  aTCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCTCCCTTCAACGGCAACTTCACTCATAGAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTggg           <  1:350376/251‑1 (MQ=255)                                                                                                                                                    ncccACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTggg           >  1:299954/2‑249 (MQ=255)                                                                                                                                                          tctctGAGTGCAGCGTCTTCCATGGTGGGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAatgatg                                                                                                                                                             <  1:234333/97‑1 (MQ=255)                                                                                                                                                             ctGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGGAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCGTTGCGCCCTACAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCg  <  1:249616/249‑1 (MQ=255)                                                                                                                                                                                       ttgtAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACGTAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAgg                                                                                                                <  1:190671/113‑1 (MQ=255)                                                                                                                                                                                          tAAAAACACGACAACCAGTAGGGCTANCAGTTGNNTGNCGATCAACGTCACATACTGNAANNNTNNNTNCAAACNTTNNCGCCCTTCANCGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATNATCCCTGAANNAAACGAGCGAGAAGCAATAATNCCGNNGNNCNCGTNccn                             >  2:120190/1‑192 (MQ=255)                                                                                                                                                                                                     cAACCAGTAGGGCTAGCAGTTGTCTGCCGATCAACGTCACATACTGAAATGATGCGCAAAAACCTTTgcgc                                                                                                                                            <  1:138837/71‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                         AATGCACGGCGATTGCGCCATAATCCTTTCAGAGATCCAGCTTCTTTTAAAGCGCGCGTTTCTTGTTGCGAAGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCG  >  NC_000913/2725013‑2725417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑