Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,880,763	G→A	R96C (CGT→TGT)	casD ←	type I‑E CRISPR system Cascade subunit CasD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,880,763	0	G	A	100.0%	37.3 / NA	14	R96C (CGT→TGT)	casD	type I‑E CRISPR system Cascade subunit CasD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/6);  total (8/6)

GATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTGATAACGCCTGTAATGAAGAAGTATCATCACGTTGGATCCCAAGACAAGCCCCGAGTAGCCCTAATAACCCGCTTCGGGTCGGAAATCTTCCGGTAGGTCGCGTTCCTTCAAAGGTCGGC  >  NC_000913/2880550‑2880995                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         gATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACAGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATc                                                                                                                                                                                                      >  1:159980/1‑250 (MQ=255)           cTGACATGTCCCCAAAAAACGCGGGTGTGCTGGTGGGCAACTTCTCCGCCCCAGGTCAGGTGTATACCGAGGCTTTCATAGTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGACAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACAGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCg                                                                                                                                                                                           >  1:167915/1‑251 (MQ=255)                                                                                                   tCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCCACGAGCGACGATAACGGAGGCATCACCTAAATATTCGCGACATGCTTGAATCGTTTCATGACTTTTCAAACCCCAGTAATCTTCTCGCGCTCCACGGACTCTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTGATAACGCCTGTACTGAAGAAGt                                                                                                   >  1:275691/1‑251 (MQ=255)                                                                                                                                      naaCCAGAGAGAGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTACATAGTTTCCTGACTTTTCAAACCACAGTAATCTTCTCGAGCTCCAAGGACAGCATGGTAATCACGCAACCACGATACAGACACACGCCGAACGTCAAGAATg                                                                                                                                                              >  1:56848/2‑157 (MQ=255)                                                                                                                                         ccAGAGAGCGACGGTAAAGGNGGCATCACANNAANATTCTNGNNNTNNNTGNATCGTTTCATGACTTTTCANACNNCAGTAANCTTCTCGCGCTCCAAGGACTGTATgg                                                                                                                                                                                                           <  2:117401/109‑1 (MQ=255)                                                                                                                                         ccAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACAGTAATCTTCTCGCGCTCCAAGGACTGTATgg                                                                                                                                                                                                           >  1:117401/1‑109 (MQ=255)                                                                                                                                          cAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACAGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTGATAACGCCTGTAATGAAGAAGTATCATCACGTTGGATCCCAAGACAAGCCCCGAGTAGccc                                                            >  1:31055/1‑251 (MQ=255)                                                                                                                                                            gaggCATCTCATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACAGTAAtat                                                                                                                                                                                                                                   <  1:464092/66‑3 (MQ=255)                                                                                                                                                                                  nccATGTTTGAATCGTTTCATGACTTTTCAAACCACAGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATc                                                                                                                                                      >  1:55474/2‑121 (MQ=255)                                                                                                                                                                                   ccATGTTTGAATCGTTTCATGACTTTTCAACCCACAGTAATCTTCTCGCGCTCAAAGGAATGTATGGCAATTACGCAACCCCGTTACAGACACACGACGATCGTCACGAATGAGTTCATCGCAGCGAACTGCAAATTGCACACTCTCTGATAACGCCTGTAATGAAGAAGTATCATCACGTTGGATCCCAAGACAAGCCCCGAGTAGCCCTAATAACCCCCTTCGGGTCGGAAATCTTCCGGTAGGTcgcg                   >  1:190353/1‑251 (MQ=255)                                                                                                                                                                                   ccATGTTTGAATCGTTTCATGACTTTTCAAACCACAGTAAGCTTCTCGCGCGCCAAGGACTGTATGGTAAGCACACAACCCCGTTACAGACACACGACGATCGTCAAGAAGGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTg                                                                                                                         <  1:470861/149‑1 (MQ=255)                                                                                                                                                                                            aaTCGTTTCATGACTTTTCAAACCACAGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCAGCGCAGCGCACTGCAAATTGCACActn                                                                                                                             <  1:149985/136‑2 (MQ=255)                                                                                                                                                                                                    cATGACTTTTCAAACCACAGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTGATAACGCCTGTAATGAAGAAGTATCATCACGTTGGATCCCAAGACAAGCCCCTAGTAGCCCTAATAACCCGCTTCGGGTCGGAAATCTTCCGGTAGGTCGCGTTCCTTCAAAGGTCGGc  <  1:457312/251‑1 (MQ=255)                                                                                                                                                                                                      tGACTTTTCAAACCACAGTAATCTTCTCGCGCTCCAAGGACTGGATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTGATAACGCCTGTAATGAAGAAGTATCATCACGTTGGATCCCAAGACAAGCCCCGAGTAGCCCTAATAACCCGCTTCGGGTCGGAAATCTTCCGGGAGGGCGCTTTCCTTCAAAgg       <  1:169362/244‑1 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         GATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACCATCGTTGCATGGGGTGTTAACCAGAGAGCGACGGTAAAGGAGGCATCACATAAATATTCGCGCCATGTTTGAATCGTTTCATGACTTTTCAAACCACGGTAATCTTCTCGCGCTCCAAGGACTGTATGGTAATCACGCAACCCCGTTACAGACACACGACGATCGTCAAGAATGAGTTCATCGCAGCGCACTGCAAATTGCACACTCTCTGATAACGCCTGTAATGAAGAAGTATCATCACGTTGGATCCCAAGACAAGCCCCGAGTAGCCCTAATAACCCGCTTCGGGTCGGAAATCTTCCGGTAGGTCGCGTTCCTTCAAAGGTCGGC  >  NC_000913/2880550‑2880995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑