Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	413,051	C→T	G901G (GGG→GGA)	sbcC ←	ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	413,051	0	C	T	100.0%	30.3 / NA	12	G901G (GGG→GGA)	sbcC	ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

GTTTTATGGCTGACCAGATCCGAAAGCGCCAGCGCCAGCGCCAGACTAACGAGGAAACTTTCGCCGCCGGAAAGGGTACGGGTATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGATTAGCGAGATGGACTAAATTATCCAGCGTCAGCCCCTGGGCAAACTTGCGGAATTTATCGCCCTCTTTGGAACCTATTAGCGAATTCAGATATCCCCAGTCCTCAACCTGCTGCGTCATTTGAGCAATTTGCTGCATTAAGGTTTGTTGTTGCTGACGGTT  >  NC_000913/412830‑413179                                                                                                                                                                                                                              |                                                                                                                                 gtTTTAGGGCTGACCAGATCCGAAAGCGCCAGCGCCAGCGCCAGACTAACGAGGAAACTTTCGCCGCCGGAAAGGGTACGGGTATCGCGTACCGCATCTTCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCATCCGGGTAAGTGGCTGATTAGCGAGATGGACTAAATTATCCAGCGTCAGTCCCTGGGCAAACTTGCGGAATTTATCGc                                                                                                      <  1:192381/250‑1 (MQ=255)        ggCTGACCAGATCCGAAAGCGCCAGCGCCAGCGCCAGACTAACGAGGAAACTTTCGCCGCCGGAAAGGGTACGGGTATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGATTAGCGAGATGGACTAAATTATCCAGCGTCAGTCCCTGGGCAAACTTg                                                                                                                   >  1:390254/1‑230 (MQ=255)                                       cgccagACTAACGAGGAAACTTTCGCCGCCGGAAAGGGTACGGGTATCGCGTACCGCATATGCCTGCCAGGTATACCCAACCTCGAATTCCAGCGCCTCGCTGGCTTTGCGCTGTAACTGATAGCGCCCGGGCAGCCGGGTAAGTTGCTGATTAGCGAGATGGACTACATTATCCAGCGTCAGTCCCTGGGCAAACTTGCGGACTTTATCGCCCTCTTTGGAACCTATTAg                                                                                   >  1:345979/1‑231 (MQ=255)                                       cgccagACTAACGAGGAAACTTTCGCCGCCGGAAAGGGGACGGGTATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGATTAGCGAGATGGACTAAATTATCCAGCGTCAGTCCCTGGGCAAACTTGCGGAATTTATCGCCCTCTTTGGAACCTATTAGCGAATTCAGATATCCCCAGt                                                               <  1:285366/251‑1 (MQ=255)                                                               gccgccGGAAAGGGTACGGGTATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGATTAGCGAGATGGACTAAATTATCCAGCGTCAGTCCCTGGGCAAACTTGCGGAATTTATCGCCCTCTTTGGAACCTATTAGCGAATTCAGATATCCCCAGTCCTCAACCTGCTGCGTCATTTg                                         >  1:379691/1‑249 (MQ=255)                                                                                              gCATCTGCCTGCCAGGGATCAACAACCTCAACTTCCAGCGCATCGCTGGCTTTGCGCTGTAACCGATAGCGCCCGTGAAGCCGGGTAAGTTGCTGATTAGCGAGATGGAATAAATTATCCAGCGTCAGTCCCTGGGCAAACTTGCGGAATTTATCGCCCTCTTTGGAACCTATTAGCGAATTCAGCTATCCCCAGTCCTCAACCTGCTGCGTc                                              >  1:475489/1‑213 (MQ=255)                                                                                                    gcctgccAGGTATCAACCACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTCAGTTGCTGATTAGCGAGATGGACTAAATTATCCAGCGTCAGTCCCTGGGCAAACTTGCGGAATTTATCGCCCTCTTTGGAACCTATTAGCGAATTCAGATATCCCCAGTCCTCAACCTGCTGCGTCATTTGAGCAATTTGCTGCATTAAGGTTTGTTGTTGCTGACGGtt  >  1:409930/1‑251 (MQ=255)                                                                                                                   acaacCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGATTAGCGAGATGGACTAAATTATCCAGCGTCAGTCCCTGGGCAAACTTGCGGAATTTATCGCCCTCTTTgg                                                                                             <  1:264454/145‑1 (MQ=255)                                                                                                                                   gcgcCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGATTAGCGAGATGGACTAAATTATCCAGCGTCAGTCCCTGGGCAAACTTGCGGAATTTATCGCCCTCTTTGGAACCTATTAGCGAATTCAGATATCCCCAGTCCTCAACCTGCTGCGTCATTTGAGCAATTtgc                               <  1:403788/191‑1 (MQ=255)                                                                                                                                                              gATAGCGCCCGTGCAGCAGGGTAAGTTGCTGATTAGCGAGATGGACTAAATTATCAAGCGTCAGTCCCTGGGCAAAATTGCGGCATTTa                                                                                                          >  1:409676/1‑89 (MQ=255)                                                                                                                                                                             gCCGGGTAAGTTGCTGATTAGCGAGATGGACTAAATTAGCCAGCGTCAGTCCCTGGGCAAACTTGCGGAAGTTATCGCCCTCTTTGGAACCTATTAGCGAATTCAGATATCCCCAGGCCTCAAACTGCTGCGTCATTTGAGCAATTTGCTGCATTAAGGTTTGTTGTTGc          <  1:490734/170‑1 (MQ=255)                                                                                                                                                                                              nnaGNNNNNTGGNCTAAATTATCCAGCGTCAGTCCCTGGGCAAACTTGCGGAATTTATCGCCCTCTTTGGAACCTATTAGCGAATTCAGATATCCCCAGTCCTCAACCTGCTGCGTCATTTGAGCAATTTGCTGCATTAAGGTttg                 <  2:309519/144‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                 GTTTTATGGCTGACCAGATCCGAAAGCGCCAGCGCCAGCGCCAGACTAACGAGGAAACTTTCGCCGCCGGAAAGGGTACGGGTATCGCGTACCGCATCTGCCTGCCAGGTATCAACAACCTCGACTTCCAGCGCCTCGCTGGCTTTGCGCTGTAACAGATAGCGCCCGTGCAGCCGGGTAAGTTGCTGATTAGCGAGATGGACTAAATTATCCAGCGTCAGCCCCTGGGCAAACTTGCGGAATTTATCGCCCTCTTTGGAACCTATTAGCGAATTCAGATATCCCCAGTCCTCAACCTGCTGCGTCATTTGAGCAATTTGCTGCATTAAGGTTTGTTGTTGCTGACGGTT  >  NC_000913/412830‑413179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑