Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,347	T→A	intergenic (+138/‑131)	metA → / → aceB	homoserine O‑succinyltransferase/malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,347	0	T	A	100.0%	56.8 / NA	21	intergenic (+138/‑131)	metA/aceB	homoserine O‑succinyltransferase/malate synthase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/9);  total (12/9)

GAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGG  >  NC_000913/4215104‑4215522                                                                                                                                                                                                                                                     |                                                                                                                                                                                nagcTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTataatta                                                                                                                                                                           >  1:314021/2‑250 (MQ=255)                                       ggCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGagt                                                                                                                                     <  1:412754/250‑1 (MQ=255)                                                                     cATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGcac                                                                                                       >  1:109644/1‑250 (MQ=255)                                                                              tACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTGTTTGATTTTGCAttt                                                                                                                                              <  1:182040/202‑1 (MQ=255)                                                                               ncGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTg                                                                                                                                                         >  1:132176/2‑190 (MQ=255)                                                                                   nacaTGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACaa                                                                                                     >  1:148191/2‑238 (MQ=255)                                                                                     caTGAATCCACCGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGaaaa                                                                                                             >  1:109622/1‑228 (MQ=255)                                                                                             cAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAACGATTCAGCACCTTACGTCAGGGAACTTCGGTTGCCTTTTTT‑ATTTCTGCAACGTCCCTCAGCTGGCGAATAGATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGACATTGTTTTTGATTTTGCATTTTACATGAGTAGTCTTAGTTGTGTTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTgggg                                                                               >  1:449579/1‑250 (MQ=255)                                                                                                               ctGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGGGCCTTTTTT‑ATTGCCGAAACGTACCTCAGCAGGTGAATAAATTTTATGCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGAt                                                                                                                                                       <  1:451520/160‑1 (MQ=255)                                                                                                                        ncgntcgntNNGNNATTNAGCACCTTACCTCAGGCACCTNNGTGTGCCTTNTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATNAACAAGGTANNAANTATTGNTNNNTNNNATNGAAATTGTTTTTGATTCTGNATNNTAAATGNGTAGTCTTAGTTGTGCTGAACGaaaa                                                                                                             <  2:109622/192‑1 (MQ=255)                                                                                                                          gATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGc                                                  >  1:408933/1‑250 (MQ=255)                                                                                                                                   gCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTTTATTTCCGCAACGTACCTCAGCAAGTGCATAACTTTTCTTCATATTGTTCTCAACCAGTTATCAAGTCTTTATAATTAAAATGGAAATTGTTTTTGATTTTTCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATc                                                                  >  1:360375/1‑226 (MQ=255)                                                                                                                                         cAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAgn                                  <  1:122869/251‑2 (MQ=255)                                                                                                                                         cAGCACCTTACCTCAGGCACCTTAGGGCGGCTTTTTT‑TTTTCCGAAACGGAACTCCGCAGGTGAATAAAATTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTTAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGcaca                                                                                                      >  1:164458/1‑183 (MQ=255)                                                                                                                                           gCACCTTACCTCAGGCGCCTTCGGGTGCTTTGGTT‑ATTTCTGAAACGTACCTCAGCAGGTGAATTAATTTTGTTCAGATTGTTAGCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGtt                                                                                                                                                             <  1:370565/126‑1 (MQ=255)                                                                                                                                           gCACCNNACNNNAGNNACCTTCGGGTGCCTTTTTT‑ATTTNCGAAACGTACCTCCGCAGGTGAATAAATTNTNTNCATANNGTTNTCAACAAGTTNTCAAGTATTTATAATTAAAATGGAAATTGtt                                                                                                                                                             >  2:370565/1‑126 (MQ=255)                                                                                                                                                                                    cGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCACGCATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGATGTGCTGAACGAAAAGCGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACaa    >  1:78286/1‑239 (MQ=255)                                                                                                                                                                                         nganccTNANNANNTGANTAAATTTTATTCATATTTTTANNAACAAGTTANCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGNAGTCTTAGTNNTGNTGAACNANNNGNNNACNACGATCCTTCGTTCACAGTNGGNNAGTTTTNGGATCCATGACGAGGAGCTGCACGAt                                             <  2:109644/189‑1 (MQ=255)                                                                                                                                                                                             ccTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTc                                                                       <  1:183918/163‑1 (MQ=255)                                                                                                                                                                                              cTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATACTTAAAATGGCACTTGTTTTTGCTTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGCAAAGAGCACAACGATCCTTCGTTAACAGTGGGGAAGTTTTCGGATCCATGACgagg                                                        >  1:77445/1‑177 (MQ=255)                                                                                                                                                                                               nnagnnnNNGAANAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTcac                                                                                      <  2:314021/144‑1 (MQ=255)                                                                                                                                                                                                                            gTTATCAACAAGTTATCAAGTATTTATAATTAAAATGGAAATTGTTTTTGATTTTGCCTTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCATTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAgg  >  1:330100/1‑201 (MQ=255)                                                                                                                                                                                                                                       gTTATCAAGTATTTATAATTAAAATGGAAATGGTTTTTGATTTTTCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGAtn                                                                  <  1:104495/126‑2 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                GAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGATCACGCCATACGATCTACGGCACATGAATCCAACGCTGGATTAATCTTCTGTGATAGTCGATCGTTAAGCGATTCAGCACCTTACCTCAGGCACCTTCGGGTGCCTTTTTT‑ATTTCCGAAACGTACCTCAGCAGGTGAATAAATTTTATTCATATTGTTATCAACAAGTTATCAAGTATTTTTAATTAAAATGGAAATTGTTTTTGATTTTGCATTTTAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGG  >  NC_000913/4215104‑4215522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑