| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,356,932 | G→A | T60I (ACC→ATC) | sapA ← | putative periplasmic binding protein SapA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,356,932 | 0 | G | A | 90.9% | 52.6 / NA | 22 | T60I (ACC→ATC) | sapA | putative periplasmic binding protein SapA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (10/10); minor base C (2/0); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGC > NC_000913/1356725‑1357138 | cGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAgg < 1:79480/250‑1 (MQ=255) ggAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGgtgt < 1:907/249‑1 (MQ=255) ncattcattTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCCGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGAc > 1:146220/2‑251 (MQ=255) cattcattTTACGAGTGGGAGTAAACCAGTCGGATTTTTGAAACGGAACCTCGCGCCGCAGGAGGAAGCGATAGATCGCGCAGTTGTCGAGTACTTCCAAGCTTTCGGCAAGTTCCGGCATCAGGCGCTAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGCTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGCCGCAATAGAc > 1:382764/1‑250 (MQ=255) tACGAGTGGGCGTAAACCAGTCGGTTTTGTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGGGGTCGAGTACTTCCCGGCTGTCGGCAAGTTCCGGCATCAGGCTATAGGTATAGGGATCGACATCCATCAGTCTATCATAAAACTGGGTGGCAAGGAGATCGACAATTAACCCACGGCTCGCttc < 1:156554/199‑2 (MQ=255) ngAGTAAACCAGTCGGTTTTTTGAAACTGAACATTGCGCCGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCCGCCTTGTGCAAGGTCCGGCCTCAGGCGCTAGGTATCGGGATCGACCTCCAGCCGGCGATCATAAAACTTGGCGGCAAGGATATCGACAATTAACCCGCTGCTCGCTTTGGCTGGGTTAAAGGGGTTGACTTTCCCGCTGACGCAATAGGCAAAACCGCTGTCGCGGat > 1:90013/2‑251 (MQ=255) naNGCNNGTGGNAGCGATAGGTCGCGCCGTTGTCGANTACTTCCAAGCGTTCGGCAAGTTCCGGCATCNGNCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGNCAAGGATATCNACANNTAACNCNCNGCTCGCTTTGGATGGGTTAAAGGTGTTGANTTGCCCGCTGACGCAATAGACAANNCCNNNGTNNCGGat < 2:382764/213‑1 (MQ=255) gACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGcc < 1:1280/251‑1 (MQ=255) gTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCTGCAAGGATATCg < 1:411213/100‑1 (MQ=255) cTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATACGTATAGGGATAGACATCCAGCAGTAGCTCATCAAACTGGGCGGAAAGGCTATCGACCATTAACCCAGTGCTCGCTTTGGATGGGt > 1:478887/1‑123 (MQ=255) tCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCg < 1:359984/109‑1 (MQ=255) tCCCAGCTNTNNNCNNGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTNGATCATAAAACTGGGNGGNAAGGNTATNGACAATTAACCCACTGCTCg > 2:359984/1‑109 (MQ=255) cggtctgCAAGTGCCGGCATCATGCTATAGGTAGAGGGATCGGCATCCATCAGGCGATCATAAAACTGGGCGGCAAGGATATCGTCAATTAACTCACAGCGCGCTGGGGATggg < 1:410786/108‑1 (MQ=255) nggCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGgcgc > 1:131935/2‑251 (MQ=255) ggCAAGTTCCGGCATCAGGCGATCGGTATAGGGCCCGAAATGCAGCAGTCCATCATAATACTGGGCGGCCAGGATATCGACCATTAACCCCCTGCTCGCTTTGGACGGGTTAACGGTGTTGACTTGACCGCTGACGCAACAGCCAAAACCGCTGTCGCGGATATCAGCATGc > 1:436378/1‑172 (MQ=255) ggCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGGCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGGCGCAATAGACAAAACCGCTGTCGCgg > 1:376213/1‑150 (MQ=255) tataGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACa > 1:219399/1‑119 (MQ=255) aGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTTACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAATTCCAGCAATCACCAAAATAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGc < 1:434264/251‑1 (MQ=255) tCGACATCCAGCAGTCGATCATAAAACAGGGAGGCAAGGATATCGACAATTAACCCAATGCTCGATTTGGATGGGTTAACGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCgcggc > 1:155635/1‑158 (MQ=255) gATCATAAATCTGGACGGCAGGGATANCAACATNNAANACACTGCTCGATTTGCATGNGTNNNANNNGNTGAAANGCNNGCTGACGCANTAGACAAAACCGCTGTCGCGGATATCAGCATGAGGGCGAGATTCCGGCGCGGCNATTGCAAGANNACTCCCAAGTCCAGCAATCACNCAANNANNCNatantan > 2:112041/1‑192 (MQ=255) gATCATAAAACTGGGCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGcc > 1:26882/1‑149 (MQ=255) gggCGGCAAGGATATCGACAATTAACCCACTGCTCGCTTTGGAGGTGATAAAGGTGTTGACTTGCCCTCTGACGCAATAGACAAAACCGCTGGCGCGGATAGCAGCATGCGGTGGAGATTCAGGCGCGGCGATTGCCTGaac < 1:484529/142‑3 (MQ=255) cAAGGATATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTcaca > 1:338745/1‑143 (MQ=255) | CGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGGAAGCGATAGGTCGCGCCGTTGTCGAGTACTTCCCAGCTTTCGGCAAGTTCCGGCATCAGGCGATAGGTATAGGGATCGACATCCAGCAGTCGATCATAAAACTGGGCGGCAAGGGTATCGACAATTAACCCACTGCTCGCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGC > NC_000913/1356725‑1357138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |