Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,315,384	G→A	Y129Y (TAC→TAT)	yciF ←	DUF892 domain‑containing protein YciF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,315,384	0	G	A	100.0%	63.6 / NA	21	Y129Y (TAC→TAT)	yciF	DUF892 domain‑containing protein YciF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/13);  total (8/13)

CATGGATTCAAGCATAGATTCGGCTTGCTTTTCCATTGCGTGGGCGTCACGTAACCAGTCATGATAATGTTCAATACGATTCATGAAAATGTCCTCTTTATAAAATGACTAAAAGTTAAAATTCATATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCAATAAGACCTTCCATTGCCACACATTTCATGCGCTTAATTTTCAGGTTCGATTCGGACTCCACAACTTGATCAATACGTTCAATCTGTCCATGAGTTTCCTCG  >  NC_000913/1315141‑1315627                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    cATGGATTCAAGCATAGATTCGGCTTGCTTTTCCATTGCGTGGGCGTCACGTAACCAGTCATGATAATGTTCAATACGATTCATGAAAATGTCCTCTTTATAAAATGACTAAAAGTTAAAATTCATATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGATAACCt                                                                                                                                                                                                                                               >  1:154386/1‑250 (MQ=255)                                               tCACGTAACCAGTCATGATAATGTTCAATACGATTCATGAAAATGTCCTCTTTATAAAATGACTAAAAGGTAAAATTCATAGTTCAGGCGTTATTTTCTGCTTTCTTATTTACGTTATGAATGGCCATATCAGTCTGTTTGATGTCGGTGGCCTTTTCTTCTACCAGGGTTTCTTGCAGAAGCTTCGCTGCTTTACGATAAc                                                                                                                                                                                                                                                 <  1:70020/202‑1 (MQ=255)                                                          ntCATGATAATGTTCAATACGATTCATGAAAATGTCCTCTTTATAAAATGACTAAAAGTTAAAATTCATATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCATTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACtt                                                                                                                                                                                     >  1:51373/2‑251 (MQ=255)                                                                             cGATTCCTGAAAATGTCCTCTTTATANAATGACNNAANGTTAAAATTCATATTTCAGNCTNNNTNNNCNGCTTTNTTNNTTACGTTATNAATGGCCAGATTAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTNTTTCAGAAGNNTCGCTGCTTTACGATACCCTANTTTNNcnncnagcngc                                                                                                                                                                                                                             >  2:121361/1‑189 (MQ=255)                                                                             cGATTCATGAAAATGTCCTCTTTATAAAATGACTAAAAGTTAAAATTCATATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATTAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTgg                                                                                                                                                                                                          <  1:121361/211‑1 (MQ=255)                                                                                  cATGAAAATGTCCTCTTTATAAAATGACTAAAAGTTAAAATTCATATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGcn                                                                                                                                                              <  1:125008/250‑2 (MQ=255)                                                                                  cATGAAAATGTCCTCTTTATAAAATGACTAAAAGTTAAAATTCATATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTGCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCgg                                                                                                                                                             <  1:432992/251‑1 (MQ=255)                                                                                               tctTTATAAAATGACTAAAAGTTAAAATTCATATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTCgn                                                                                                                                                                                                                           <  1:306945/176‑2 (MQ=255)                                                                                                             cTAAAAGTTAAAATTCATATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTAAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTAc                                                                                                                                  >  1:258251/1‑251 (MQ=255)                                                                                                                                   cNGNCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCANTTTGATGTCGNTGGNNTTTTNTNCNTCCAGGGTTTCTTTCAGAAGCTTCGCTGCNTTACGATAACCTAATTGTTCAGCNNGCNNNGCNNATGTc                                                                                                                                                                                                                    <  2:371941/147‑1 (MQ=255)                                                                                                                                   cAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGACCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTc                                                                                                                                                                                                                    >  1:371941/1‑147 (MQ=255)                                                                                                                                              ttCGGCTTTCGTATTTACGTTATTAATGGCCAGATCATTCAGTTTGATGTCTGTGGCCTTTTCTTCTTCCATGGTTTCTTTCAAAAGCTTCCCTTCTTTACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTc                                                                                                                                                                                          <  1:360520/162‑1 (MQ=255)                                                                                                                                                                                                  gTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTc                                                                                                                                                                                   <  1:407435/117‑1 (MQ=255)                                                                                                                                                                                                     gCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTAGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCAATAAGACCTTCCa                                                                                           >  1:351395/1‑202 (MQ=255)                                                                                                                                                                                                     gCCTTTTCTTCTTCCAGGGTTTCGTTCAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTcn                                                                                                                                                                                                                            <  1:63644/73‑2 (MQ=255)                                                                                                                                                                                                     gCCTTTTCTTCTTACAGGGTTTTTTTCAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTcag                                                                                                                                                                                                                                     >  1:144785/1‑64 (MQ=255)                                                                                                                                                                                                            cttcttcCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGAcc                                                                                                                       <  1:106868/167‑1 (MQ=255)                                                                                                                                                                                                            cttcttcCAGGGTTTCTTTCAGAAGCNTCGCTGNNTTNCGATAACCTAATTGTTCAGNCANNNTNNNTNATGTCNCGNNACTGGCAATNTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCNTTTTTCTCGNNACTTTCGATGAcc                                                                                                                       >  2:106868/1‑167 (MQ=255)                                                                                                                                                                                                                          tctttcAGAAGCTTCGCTGCTTTACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAAACTCATAATGCTCGACTGTCTGTGCTGCGGCAATCAGTGCGGCATCACTCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCAATAAGACCTTCCATTGCCACACATTTCATGCGCTTAATTTTCAGGTTCGATTCg                                                  <  1:498570/222‑1 (MQ=255)                                                                                                                                                                                                                                               ttACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTtctccg                                                                                                                                     <  1:466006/118‑3 (MQ=255)                                                                                                                                                                                                                                               ttACGATAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTGTCGATGTCCTCATTATCGTCTTCAATAAGTCCTTCCATTGCCACACAGTTCATGCGCGTAATTTTCAGGTTCGATTCGGACTCCACAACTTGATCAATACGTTCAATCTGTCCATGAGTTTCCTCg  <  1:82236/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    CATGGATTCAAGCATAGATTCGGCTTGCTTTTCCATTGCGTGGGCGTCACGTAACCAGTCATGATAATGTTCAATACGATTCATGAAAATGTCCTCTTTATAAAATGACTAAAAGTTAAAATTCATATTTCAGGCTTTATTTTCGGCTTTCTTATTTACGTTATTAATGGCCAGATCAGTCAGTTTGATGTCGGTGGCCTTTTCTTCTTCCAGGGTTTCTTTCAGAAGCTTCGCTGCTTTACGGTAACCTAATTGTTCAGCCAGCGTCGCTAATGTCCCGTAACTGGCAATCTCATAATGCTCGACTTTCTGTGCTGCGGCAATCAGTGCGGCATCACGCACTTCGTTTTTCTCGGTACTTTCGATGACCTCATTAGCTTCTTCAATAAGACCTTCCATTGCCACACATTTCATGCGCTTAATTTTCAGGTTCGATTCGGACTCCACAACTTGATCAATACGTTCAATCTGTCCATGAGTTTCCTCG  >  NC_000913/1315141‑1315627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑