Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,604,840	C→T	S265F (TCC→TTC)	lsrD →	Autoinducer‑2 ABC transporter membrane subunit LsrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,604,840	0	C	T	100.0%	53.2 / NA	20	S265F (TCC→TTC)	lsrD	Autoinducer‑2 ABC transporter membrane subunit LsrD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/11);  total (9/11)

GCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCG  >  NC_000913/1604602‑1605064                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 gCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCAc                                                                                                                                                                                                                      >  1:178430/1‑251 (MQ=255)                                  ccGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCCGACCTTATGTTCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTCTTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGC‑CCATATTTATGGTGG‑TTCCGGGTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGc                                                                                                                                                                                      >  1:199565/1‑250 (MQ=255)                                       ggctcttccgatcggcgcGATTCAAGTTAACCGTATCTGAGGGGGGCTCTATGCCA‑GGTGGGGCTGGCGGCTGGGTTCGCCGCGGTGGTGCTGGTATCGTATTTTGGTCCAGCACGTCCCGATCGCGGGGCGTCGTTTCTGAGGCCCGCCATGACCGCCGTGGTGCTTGGCGGGGC‑CAATATTTA‑GGTGGGTTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAgg                                                                                                                                                                               <  1:371032/237‑1 (MQ=255)                                              ttttATTGCGCTATTCCAGTTACCCTTACCTTTTGTGCTCTCTAGGCCATGGCGGGGCTGGGTTCTGCGGTCGCCGCTGTGTTGCTGGTATCGTATTTTGGTTCAGCCCGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCTGCACCGCCTTGGTGCTTGGCGGGGC‑CAAGATTTATGGGGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGc                                                                                                                                                                          <  1:340545/249‑1 (MQ=255)                                                       gcgATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAgg                                                                                                                                                                >  1:17298/1‑251 (MQ=255)                                                                                  gcgcTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTTTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTg                                                                                                                                                                                       >  1:1201/1‑201 (MQ=255)                                                                                  gcgcTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGATGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCCCCGCCGTGGTGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCt                                                                                                                                     >  1:488891/1‑251 (MQ=255)                                                                                                            gTCTGCGGTCGCCGCTGTGCTGCTGGCATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGTGTCGTTTCTGCTGCCCGACATCACCGCCGTGGCGCTTGGCGGGGCACCAT‑TTTATGGTGG‑TTCCGGTTTAATTATCGGCCCCGCCATTGCGGTTTTATTGGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAg                                                                                                                                                              >  1:468494/1‑200 (MQ=255)                                                                                                                ncGGTCGCCGCTGTGCTGCTGGTATCGTCTTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTgg                                                                                                                                                                                               >  1:148136/2‑163 (MQ=255)                                                                                                                                    ggttttggattttgttccagccctttCCGAGCTCGGTGTGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTTCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGc                                                                                   <  1:388558/227‑1 (MQ=255)                                                                                                                                                  gTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCCCCATTTCGGTTTTATTAGTGGGATANTTGNNNNNAg                                                                                                                                                                                >  2:308370/1‑144 (MQ=255)                                                                                                                                                                     cGGTGCGTCGTTTCTGATCCCCTCCATCTCCGCCGTGGGGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCCGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGtggctg                                                         <  1:407667/244‑1 (MQ=255)                                                                                                                                                                         gctttgtTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGC‑TAATATTGATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCGTTCCGGTGCGCTACTTAGCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCgggn                                              <  1:54399/246‑2 (MQ=255)                                                                                                                                                                          cgtcgtTTCTGATGCCCGCCACCACCGCCGTGGTGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCgggn                                              <  1:308370/250‑2 (MQ=255)                                                                                                                                                                                    gATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGtn                                                  <  1:149438/236‑2 (MQ=255)                                                                                                                                                                                                          gtgCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGGCGTTGTCGTAGGTCGTTCCGTTAGCCTGc                                                                                   <  1:401567/181‑1 (MQ=255)                                                                                                                                                                                                           tgCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAgn                        <  1:148355/239‑2 (MQ=255)                                                                                                                                                                                                            gCTTGGCGGGGC‑CAATATTTATGTTGG‑TTCCGGTTTCATTATCGGCCCCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAATGATATCTTCATGnc           <  1:395281/251‑1 (MQ=255)                                                                                                                                                                                                                     ggC‑CAATATTTATGGTGG‑TTCCGGTTTCATTATCGGCACCGCCATTGCGGTTTTCTTAGTGGGATATTTGCCACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTCCTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCg  >  1:68380/1‑251 (MQ=255)                                                                                                                                                                                                                                  tggtgt‑TTCCGTTTTCATTATCGGCACCGCCAGTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTGGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGTCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGac           <  1:374509/230‑1 (MQ=255)                                                                                                                                                                                                                                  tggtgn‑NTCNNNTTNNATTATCGGCACCGCCATTGCGGTNTTATTAGTGGGATATTTGCAACAAGGTTTNCNANTGGCNNGAGNGCCAAATCAGNTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCNTNCCGTTAGCCTGCATCGCCAGCAAATTAAAGANTGGCTGGCGCGTCGGGCCAATaaanccttnnccnannnnnnn                     >  2:374509/1‑201 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                 GCCGGACGTAATGTGTTTTTGATTGGGCAAAGCCCGCGCGTGGCGCTTTATAGCGCGATTCCAGTTAACCGTACCTTATGTGCGCTCTATGCCATGACGGGGCTGGCGTCTGCGGTCGCCGCTGTGCTGCTGGTATCGTATTTTGGTTCAGCACGTTCCGATCTCGGTGCGTCGTTTCTGATGCCCGCCATCACCGCCGTGGTGCTTGGCGGGGC‑CAATATTTATGGTGG‑TTCCGGTTCCATTATCGGCACCGCCATTGCGGTTTTATTAGTGGGATATTTGCAACAAGGTTTGCAAATGGCAGGAGTGCCAAATCAGGTGTCCAGCGCCCTTTCCGGTGCGCTACTTATCGTCGTTGTCGTAGGTCGTTCCGTTAGCCTGCATCGCCAGCAAATTAAAGAGTGGCTGGCGCGTCGGGCCAATAACCCATTGCCATAAAGGATATCTTCATGACACTTCATCG  >  NC_000913/1604602‑1605064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑