Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	633,227	C→T	G84D (GGT→GAT)	hcxA ←	hydroxycarboxylate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	633,227	0	C	T	100.0%	41.9 / NA	15	G84D (GGT→GAT)	hcxA	hydroxycarboxylate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/4);  total (11/4)

TGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACATCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGT  >  NC_000913/632997‑633470                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    tGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCATCGGACTCAg                                                                                                                                                                                                                                            <  1:249905/240‑1 (MQ=255)           ctcCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGGGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCATCGGACTCAGCCGCCAGTTGTTGTACa                                                                                                                                                                                                                           >  1:31193/1‑247 (MQ=255)                                                       cATAATGCAGCTCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGGCCAGGCGGCGCAGGTGGCGGCGATCGTCGTAACGGCAACAAACGGCAGACCGAGACGGTGGGCGAGGGCTGGCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCGCCACGCTGCGGTCTTCATCGGACTCAGCCTCCAGTGTTTGTACATCGCTTTCGCTGCAATGACCGCGAAACAAAATAGGCTTTGCGCCTgtc                                                                                                                                                                           <  1:71377/251‑3 (MQ=255)                                                                                                                        gcggcgTTCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGGGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCATCGGACTCAGCCGCCAGTTGTTNTACNNNNNtt                                                                                                                                                                                                                     >  2:298217/1‑144 (MQ=255)                                                                                                                           gcgATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCATCGGACTCAGCCGCCAGTTGTTGTACATCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCgcagca                                                                                                                                    >  1:222546/1‑222 (MQ=255)                                                                                                                              aTCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCCCCGCCACCGACGCCAATCACCACGCTGCGGTCGTCATCGGACTCAGCCGCCAGTTGTTGTACATCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACcgcgcn                                                                                                    <  1:298217/251‑2 (MQ=255)                                                                                                                                                                ggcgggcgNGNNNTNNAGCGGTGTCGAGCAGTGCACCGCCACCGACGACAATCACCCCGCNGCGGTCGTCCTAGGANTCNGCCGNCGGNTGTTGTACATCGCTTTCGCTGCAATGAc                                                                                                                                                                                                        >  2:347773/1‑117 (MQ=255)                                                                                                                                                                         gggCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCATCGGACTCAGCCGCCAGTTGTTGTACATCGCTTTCGCTGCAATGACCGCGAAGCAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTgcgn                                                                                                                                           <  1:302369/169‑2 (MQ=255)                                                                                                                                                                         gggCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCATCGGACTCAGCCGCCAGTTGTTGTACATCGCTTTCGCTGCAATGACCGCGAAGCAAAATATGCTTTGCCCCNGGCNNNNNa                                                                                                                                                                     >  2:302369/1‑143 (MQ=255)                                                                                                                                                                                cgaggTGTCGAGCAGTGCACCGCCACGGACGCCAATCACCCCGCTGCGGTCGTCATCGGACTCAGCCGCCAGTTGTTGTAAATCGCTTTCTCTGCAAt                                                                                                                                                                                                           >  1:72578/4‑98 (MQ=255)                                                                                                                                                                                                 cACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCATCGGACTCAGCCGCCAGTTGTTGTACATCGCTTTCGCTGCAATGACCGCGACACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATg                                                                                                                                 >  1:264842/1‑155 (MQ=255)                                                                                                                                                                                                     gCCACCGACGCCAATCACCACGCTGCGGTCGTCATCGGACTCAGCCGCCAGTTGTTGTACATCGCTTTCGCTGCAATGACCGCAAAACAAAATATGCTTTGCCCCTGGCAGTCCACACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCg                             >  1:443299/1‑251 (MQ=255)                                                                                                                                                                                                              gCCAATCACCACGCTGCGGTCGTCATCGGACTCAGCCGCCAGTTGTTGTACATCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCcgacg                     >  1:284357/1‑250 (MQ=255)                                                                                                                                                                                                                                ntcgtcATCGGACTCAGCCGCCAGTTGTTGTACATCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGGGAAAag                                       >  1:306195/2‑214 (MQ=255)                                                                                                                                                                                                                                gtcgtcATCGGACTCAGCCGCCAGTTGTTGTACATCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTCGCCGGGCCGACGACCACGCGGCTAGGATtgt  >  1:221706/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    TGAGGATAATCTCCGGTTCCACCAGCACCATAAAATTGGCGTCGTCGAAAATCTCATAATGCAGCGCCTGTCCGGCATCATTATACCAGACGGAGAGCGGTGTCCAGGCGGCGCAGGTGGCGGCGATCGTCGGAACGGCAACAAACGGCAGACCGAGACGGCGGGCGAGGGCTTTCGCGGTGTCGAGCAGTGCACCGCCACCGACGCCAATCACCACGCTGCGGTCGTCACCGGACTCAGCCGCCAGTTGTTGTACATCGCTTTCGCTGCAATGACCGCGAAACAAAATATGCTTTGCCCCTGGCAGTCCAAACGCTGGCGGAAGTTTGGTTTGCGCCGCAGCAATGGCGCGTTTGCCGTAGATCCACACCGCGCGAGAAAGTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGT  >  NC_000913/632997‑633470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑