| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 294,093 | G→A | P236L (CCT→CTT) | yagM ← | protein YagM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 294,093 | 0 | G | A | 94.4% | 47.1 / ‑6.1 | 19 | P236L (CCT→CTT) | yagM | protein YagM |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (9/8); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGGCAGCATAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGAGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTAT > NC_000913/293866‑294337 | gagagTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGGCAGCATAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAACGCATAACAATCCCCTTtcttct > 1:330488/1‑250 (MQ=255) gATTGTCAGCGATACTTTAACTGGTAAAGTAGTATTTGACCTCTCATAATTGTTTGTCCTTCTTTTTTACCTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGGCAGCATAACCACCATTTCATTGACATGGTAAAGATCAATCCAACAAACAACCGGATAAAGCATAACAATCCCCTTTCTTCTAAGAAc < 1:356428/250‑1 (MQ=255) gCCAGCGATACTTTACCTGGTAAAGTAGCATTTTACCTCTCATAATTGTTTGTCCTTTTTTGTTACCTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATACGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGGCAGCATAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAAGCATAACAATCCCCTTTCTTCTAAGAACTTCAt > 1:156144/1‑251 (MQ=255) aTTTTACCTCTCATAAGTGTTTGTCCTTCTTTGTTACCTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAAGGATGTCATCCGGTACATTAACACGGTTGGAAACATCGGTTTTGCGGTGCACTTGCTGCATAAGCACCAGTTCATTGACATGGTAAATAGCAAGCCAACAAACATCCGGGTGAAGTTGAACAATCCCCTTTCTGCTAAGAACTTCATTCATcccaattgattatattcgggataa < 1:450362/250‑22 (MQ=255) ttACCTCTCATAATTGTTTTTCCTTCTTTGTTACCTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTTCATTAACACGGTTGAAAACATCTATTTTGCGTAGCACTGGCAGCATAACCACCATGTCATTGACATGGTAAATATCATTCCAACAAACAACCGGATAAAGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGa < 1:503017/250‑1 (MQ=255) gtCCTTCTTTGTTACCTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGGCAGCATAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAAGCATAACAATCCCCTTTCTTCTAAGATCTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTagagn < 1:98748/243‑2 (MQ=255) ttACCTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTGTTGCGGAGCACTGGCATCATAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAAGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTagagac < 1:456771/233‑3 (MQ=255) ccTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGGCAGCATAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAAGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGAGCCTGCCCGGCATCAAAGtaata > 1:321402/1‑250 (MQ=255) ctTAGGTTNTNNNCNNTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACNGTTGGAAACATCTGTNTTNCGGANCACNGGCAGCATAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAAGCATAACAATCCCCTTTCTTCTAAGAACTTCa > 2:358173/1‑182 (MQ=255) gatgTCATCCGGTACATTAACACGGTTGGAACCATCTGTTTTGCGGAGCACTGCCAGCATAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAAGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATc > 1:201478/1‑151 (MQ=255) ggTACATTAACCCTGTTGGAAACATCTTTTGTGCGGAGCACTGGCAGCATAACCACCATGTCATTTACATGGTAAATATCATGCCAACAAACAACCGCATAAATCATAACAATCCCCTTTCTGCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATg < 1:341766/163‑1 (MQ=255) gTTGGAAACATCTGTTTGGCGGAGCACTGGCAGCATAACCACCATGTCATTGACATGGTAAATATTAATCCAACAAACAACCGGATAAAGCATAGCAATCCCCTTTCTTCTAAGAACGTCATTTATCCCGAATATAATCAATGGTGATGTGGACAAGAGTAGAGCCTGCCCGTCATCAAAGt < 1:321208/182‑1 (MQ=255) ccaTTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAAGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGAAGAGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTcc > 1:382098/1‑251 (MQ=255) cATTGACATGGTAAATATCAATCCAACAAACAACCGGATACAGCATAACCCTCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGAGcc > 1:246164/1‑119 (MQ=255) atatCAGTNCNNNANNCAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCANTTATCCCGAATATAANCANTTGGNATGNTGACAAGAGTAGAGc > 2:346057/1‑104 (MQ=255) anttNAATCCNACAANCAACCGGATAAAGCANAACAATCCCCTTTCTTCTANNANCTTCANTTATc < 2:201478/63‑1 (MQ=255) aaacaaCCGGATAAAGCATAACAATCCCCTTTCTTCTAAAAACTTCATTAGTCCCGAatat > 1:450645/1‑61 (MQ=255) ncGGATAAAGCATAACAATCCCCTTTCTTCTAAGAACTTGATTTATCCCGAATATAATCAATTGGGATGTTGACAag > 1:50852/2‑77 (MQ=255) gATAAAGCATAACAATCCCCTTTCTTCTAAGAACTTCATTGATCCCGAATATAATCATTTGGGATGTTGACAAGAGAAAAGCGTGCCCGGCATAAAAGCAATAACCGTCAAAGGATGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCACAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGtat > 1:229785/1‑250 (MQ=255) | GAGAGTGATTGCCAGCGATACTTTAACTGGTAAAGTAGTATTTTACCTCTCATAATTGTTTGTCCTTCTTTGTTACCTCTTAGGTTCTGCTCTCTTGTGAGTATGGGTTTATAATGATGTCATCCGGTACATTAACACGGTTGGAAACATCTGTTTTGCGGAGCACTGGCAGCATAACCACCATTTCATTGACATGGTAAATATCAATCCAACAAACAACCGGATAAGGCATAACAATCCCCTTTCTTCTAAGAACTTCATTTATCCCGAATATAATCAATTGGGATGTTGACAAGAGTAGAGCCTGCCCGGCATCAAAGTAATAACCCTCAAAGGTTGTTCTATGAATAAGAATAATGTTTGAATATCCTGAGACACTTTGATGCTTTACATCAAAATGTCTTTGATTGTAAATGTAAATGGCTTTTTCCATATCGTAATTTTCCCTTAGCGAGGATAGCCTTTCACGTAT > NC_000913/293866‑294337 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |