Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,705,623	G→A	L56L (CTC→CTT)	dppB ←	dipeptide ABC transporter membrane subunit DppB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,705,623	0	G	A	100.0%	25.4 / NA	12	L56L (CTC→CTT)	dppB	dipeptide ABC transporter membrane subunit DppB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/2);  total (10/2)

CGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCTATAGTGACCCGCAGCC  >  NC_000913/3705436‑3705820                                                                                                                                                                                            |                                                                                                                                                                                                      cGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCAAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATc                                                                                                                                                                <  1:276480/227‑1 (MQ=255)        cGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGCAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGCAATGCCTCGATCGCCATGCATAACGCCCCAACTGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCAAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGAt                                                                                                                                 >  1:270787/1‑251 (MQ=255)         ggAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCAAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATc                                                                                                                                >  1:800/1‑251 (MQ=255)                                                                ccTGGAAGCGCGGCACAAACTCTTCCCAACCCGGGATGCGGCTTTTCATTGAAATGCCTAGCTCGCCATGCAAAACCCCCCAAATGTAATGGAGAGACTCCTGCCAGATGGTTTTATCTAGGCCAAGTTCACCAAGCAGCTGCCCGTGACGCTCTGGGGATACCCCCCGTCCCCCCGCCATGATCAGCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGt                                                                                         <  1:72011/235‑1 (MQ=255)                                                                             cACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGAAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCAAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTc                                                            >  1:373240/1‑251 (MQ=255)                                                                              aCGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCCCCTCGGTTTATCTAAGCCAAGTTCAGCCAGCAGCTgcg                                                                                                                                                                                    >  1:272373/1‑130 (MQ=255)                                                                                 aaCTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATACCTAGATCGCCCTGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCAAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAgg                                                                                                         >  1:432849/1‑202 (MQ=255)                                                                                                gggATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTCAGCCAAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAggg                                                                                          >  1:435731/1‑202 (MQ=255)                                                                                                                  gAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCAAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTcgcccgc                                                                                                                                                  >  1:421317/1‑128 (MQ=255)                                                                                                                                                     ntAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCAAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCTATAGTGACCCGCAGcc  >  1:309946/2‑237 (MQ=255)                                                                                                                                                                               gTTTANCTAAGNCNNGTTCAGCCAGCAGCTGCGCNTGACGCTCTGGGGAGNTCCCNCGTTcnccng                                                                                                                                                   >  2:214929/1‑66 (MQ=255)                                                                                                                                                                                tttATNNAANNNACNNTCAGCCAGCAGCTGCGAGTGACGNTCTGGGGAGATCCCACGTTCGACCGACATNANCNTCACNNGATNGCCCGGGATCNTGTGGACAAAGGCCAATGTGAGCAGGGTAATACCGATAAACGGGGGGCNGNAGAGTc                                                            >  2:382936/1‑152 (MQ=255)                                                                                                                                                                                      tAAGCCAAGTTAAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGATCGCCCGCCATGATCATCACCGGCTCGACCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTcc                                                           >  1:425295/1‑147 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                                      CGCCGACCGGAATACCGACTGCCGTAGCAAAAATCATCGCGCAGACGCCAAGTTCCAGCGTGGCCTGGAAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTTCAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTTCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACATAACCCGGATTCTCTATAGTGACCCGCAGCC  >  NC_000913/3705436‑3705820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑