| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 186,539 | C→T | T704T (ACG→ACA) | glnD ← | protein‑PII uridylyltransferase/uridylyl‑removing enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 186,539 | 0 | C | T | 96.4% | 67.2 / ‑3.8 | 28 | T704T (ACG→ACA) | glnD | protein‑PII uridylyltransferase/uridylyl‑removing enzyme |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (10/17); total (11/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
CAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCC > NC_000913/186334‑186769 | cAAACCGAATAACCTCATGACGATCTGCGCACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACCGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCCGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGc > 1:154060/1‑251 (MQ=255) aaCCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGGGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGTCGGTCCGTGCTCCAGATAAAAATCGCGGTGCCTCCGCGTGTAGCCTGCGGGCTAAGCAATACCAGCTGTTTGCTTAAATCATGCTg < 1:203957/251‑1 (MQ=255) cATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCgg < 1:178487/251‑1 (MQ=255) gCGGACAGCGGGTTGCAATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGGGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATgccag < 1:273114/249‑1 (MQ=255) gTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATGCCAGCGGTTTGCTTAAATCATGc < 1:420804/214‑1 (MQ=255) ncATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTg > 1:130518/2‑212 (MQ=255) ccAGCACGATAAAGGTATCCATCGCCATACCGGCGCGAGTGGTGAAAATTTGGGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACATATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCGTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAAGCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGAc < 1:266856/250‑1 (MQ=255) ccATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGATGTAATAAATGGCGGGGATGCCCAGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACa > 1:25055/1‑250 (MQ=255) gtgAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGc < 1:332383/199‑1 (MQ=255) aTTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATTCCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGTGCATGCCAGGCCAGTTGAt < 1:439680/187‑1 (MQ=255) gCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGc < 1:403896/164‑1 (MQ=255) ggTCTAAGTCGGCACAGACGGCGGCAAACAGAGAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGc < 1:396453/162‑1 (MQ=255) aTTCGTCACAGACGGCGGCAAACAGATAAGGGCGTTCCGGGCGCCAGATAAAAATCTCTGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCgg < 1:367031/100‑1 (MQ=255) aTTCGNNACNNNCGNNGGCAAACAGATAAGGGCGGTCCGNGCTCCAGATAAAAATCTCGGTGCATCCACNTNTNGCCTNNGGGNTAAGCAATACNAGCgg > 2:367031/1‑100 (MQ=255) cGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAATTCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAATGCCTGGAGt > 1:295143/1‑251 (MQ=255) ggCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATcc > 1:169373/1‑223 (MQ=255) gCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTg > 1:467686/1‑251 (MQ=255) gACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTTCACGTGTAGCCTGCGGGCTAAGc > 1:191410/1‑79 (MQ=255) aNATNAGGGCNGTCCNGGCTCCAGATAAAAANCTCGGTGCCTCCACGTGTTNNCNGCGGGNTAAGc < 2:191410/66‑1 (MQ=255) gATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCTTGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGAc < 1:41512/167‑1 (MQ=255) gATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATANNGCTGtanna > 2:41512/1‑99 (MQ=255) ggTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCa < 1:472121/246‑1 (MQ=255) gTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATNNATGGCGNNc > 2:32909/1‑99 (MQ=255) gTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATgccaggcc < 1:32909/109‑1 (MQ=255) aaaaTCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTATGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTcc < 1:494800/251‑1 (MQ=255) aaaaTCTCGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTAGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTcc < 1:494774/251‑1 (MQ=255) tctcGGTGCCTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCGTAATTCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTa < 1:438812/122‑1 (MQ=255) nccTCCACGTGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGAt > 1:126162/2‑200 (MQ=255) | CAAACCGAATAACCTCATGACGATCTGCGGACAGCGGGTTGCCATCGGGTTCCAGCACGATAAAGGTATCCATCGCCATACCGTCGCGAGTGGTGAAAATTTGTGCGTCGTGAACACTTAAATTGCGGCGGTCTAATTCGGCACAGACGGCGGCAAACAGATAAGGGCGGTCCGGGCTCCAGATAAAAATCTCGGTGCCTCCACGCGTAGCCTGCGGGCTAAGCAATACCAGCGGTTTGCTTAAATCATGCTGTAATAAATGGCGGGCATGCCAGGCCAGTTGATTTGGGCTATGGCGGACAAAATAGTTAGCACGACAGCGTGACCAAATTTGGTGCAGCGCCTCTTCGTCGATGTTATCCATGCGCAGTAGTGCCAGTGCCTGGAGTTGGTGATGGCGAACCCGTTCGCGCATATCCGGCGTGTTTTGCATTCC > NC_000913/186334‑186769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |