Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,996,049	T→A	L156* (TTG→TAG)	yigA →	DUF484 domain‑containing protein YigA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,996,049	0	T	A	100.0%	40.5 / NA	15	L156* (TTG→TAG)	yigA	DUF484 domain‑containing protein YigA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (7/8);  total (7/8)

AACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTTGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAAC  >  NC_000913/3995812‑3996282                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          aaCAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAAc                                                                                                                                                                                                                                >  1:425096/1‑249 (MQ=255)                                                         ctcACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGa                                                                                                                                                                                                                                  >  1:202156/1‑191 (MQ=255)                                                                                                                                aGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGc                                                                                                                                                                                                                 <  1:321719/137‑1 (MQ=255)                                                                                                                                              gcCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGTAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTg                                                                                                                                                               <  1:340715/173‑1 (MQ=255)                                                                                                                                                             gCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCCTTAAGCCGTAAGTCTTTCGCAACGCTGCGTATTCAGCGTTAGGGGCAGGa                                                                                                                                                                                                                                  >  1:349079/1‑91 (MQ=255)                                                                                                                                                             gCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGAGGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGt                                                                                                        <  1:421187/213‑1 (MQ=255)                                                                                                                                                             gCNGGCNCTTANGTNCGCCGTCGAACCACANTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCNNCNNNANGGGCAGGa                                                                                                                                                                                                                                  <  2:349079/91‑1 (MQ=255)                                                                                                                                                                    cTTAGGGGCGCCGTCGAACAACACTCCTCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGAAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCAATATCAACAAGGGCAAGGAACGc                                                           >  1:428293/1‑251 (MQ=255)                                                                                                                                                                                      cnACNCTCATNTGGCNTTAAGCCGTCAGTCTNTCGAACCGCTGCGTATTCANNGNGAGGGNCAGGa                                                                                                                                                                                                                                  <  2:202156/66‑1 (MQ=255)                                                                                                                                                                                                    cATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTcgcg                                                                                            <  1:391680/186‑1 (MQ=255)                                                                                                                                                                                                               cAGTCTTTCGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGTCGATTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAggg                                                                     >  1:293788/1‑198 (MQ=255)                                                                                                                                                                                                                       cGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTgg                                                                                                                                                                                                                   >  1:33507/1‑48 (MQ=255)                                                                                                                                                                                                                       cGAACCGCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTgg                                                                                                                                                                                                                   <  2:33507/48‑1 (MQ=255)                                                                                                                                                                                                                             gCTGCGTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAAc  >  1:279935/1‑251 (MQ=255)                                                                                                                                                                                                                                  gTATTCAGCGTTAGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGc                                                                                                                                                                   <  1:79065/85‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          AACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTTGGGGCAGGAACAGCACTATCTTGGGCCGCTTAACGGACCAGAGCTGCTGGTGGTGCTACCGGAAGCGAAAGCGGTGGGATCGGTGGCGATGTCGATGCTGGGAAGCGATGCTGATTTGGGTGTCGTGCTGTTTACCAGTCGCGATGCCAGTCACTATCAACAAGGGCAAGGAACGCAGTTACTTCATGAAATTGCGCTGATGTTGCCGGAGCTTCTGGAGCGTTGGATTGAAC  >  NC_000913/3995812‑3996282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑