Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,970,539 C→T T136I (ACA→ATA)  wecB → UDP‑N‑acetylglucosamine 2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,970,5390CT100.0% 60.0 / NA 23T136I (ACA→ATA) wecBUDP‑N‑acetylglucosamine 2‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/15);  total (8/15)

GCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTACATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGCAAAACTTGCTGCGTGAAAACGTTGCGGATAGCCGAATCTTCATTACCGGTAATACAGTCATTGATGCACTGTTATGGGTGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATA  >  NC_000913/3970315‑3970745
                                                                                                                                                                                                                                |                                                                                                                                                                                                              
gCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGGTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATc                                                                                                                                                                                      <  1:7593/251‑1 (MQ=255)
   gCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACt                                                                                                                                                                                   >  1:461080/1‑251 (MQ=255)
              gCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAAcc                                                                                                                                                                        >  1:208852/1‑251 (MQ=255)
                                    gATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGCAAAACTtgn                                                                                                                                                  <  1:121622/251‑2 (MQ=255)
                                                  tAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGGGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGc                                                                                                                                                           <  1:140863/228‑1 (MQ=255)
                                                   naaaCCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGATGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTATCCAACCGAAACTTCCCGGCAAAACTTGCTGCGTGAAAACg                                                                                                                                      >  1:311994/2‑248 (MQ=255)
                                                            tCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGCAAAACTTGCTGCGTGAAAACGTTGCGGATAGc                                                                                                                           <  1:18013/250‑1 (MQ=255)
                                                                gCCGAGTTNANNNCNNACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACANGCCCGGCGGCGTTTTNTCNGCGTNTTCNTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACAggg                                                                                                                                                                                                      >  2:354317/1‑171 (MQ=255)
                                                                gCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTAATCAGCGTATTCCTGTTGGTCACGTTGATGCTGGACTGCGCGCGTGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCggg                                                                                                                                                                                                      <  1:354317/171‑1 (MQ=255)
                                                                                               aCGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGCAAAACTTGCTGCGTGAAAACGTTGCGGATAGCCGAATCTTCATTACCGGTAATACAGTCATNg                                                                                            <  1:395044/246‑1 (MQ=255)
                                                                                                cGGCTATACGACGACGACGCTGGCAACCAGGCTGGCGGCGTTTTATCAGCGTATTCCTTTTGGGCACGTTGAGGCTGTTCTGCTCAGGGGTAATCTCTATTCGCCGTGGCCGGAGGAGGCTAACCGGATATTGACCGGGCATCTGGCGATTTATCACTTCTCACCAACCGAAACTTCCCGGCAAAACtt                                                                                                                                                    <  1:223101/189‑1 (MQ=255)
                                                                                                       actactacgacgCTGGCAACCAGCCGTGCGGCGTTTTATCAGCGTATTCCTGTTGGTCGCGTTGAGGCTGGTCTTCGCCCGGTCGATCTCTATTCGCCGTGTCCGGAATAGGTTAACCGTATATTGACTGTGCAGCGGGCGATGTATCACTTCTCTCCAAACTAAACTGCCCGGCAAATCTTGCTGCGTGAAAACGTTGCGGATAGCCGAGTCTTCATTACCGGTAATTCAGTCATTGATTCACTGTTAt                                                                                <  1:414146/244‑1 (MQ=255)
                                                                                                                       cAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGTTCACGTTTAGGCTGGTCTGCGCACGGGCGATCTTTATTCGCCGTGGCTGGAAGAGGTGAACCGTATAGTGACCGGGCATCTGGCGATGTATCTCTTCTCTCCAACCGAAACTTCCCg                                                                                                                                                             <  1:341449/157‑1 (MQ=255)
                                                                                                                                 ggcggcGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGCAAAACTTGCTGCGTGAAAACGTTGCGGATAGCCGAATCTTCATTACCGGTAATACAGTCATTGATGCACTGTTATGGGTGCGTGACCAGGTGATGagcagc                                                      <  1:437104/250‑1 (MQ=255)
                                                                                                                                                 nnnnnnnnnnttNNNNNCGTNGAGGCTGGTCTGCGCACGGGCGAGCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGCAAAACTTGCTGCGTGAAAACg                                                                                                                                      <  2:311994/144‑1 (MQ=255)
                                                                                                                                                          gTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGACCGGAAGGGGGTAAACGTATATTGCCCGGGCCTCTGGCGATGTATCAGTTCTCTCCAAc                                                                                                                                                                         >  1:443639/1‑110 (MQ=255)
                                                                                                                                                             ggTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCggg                                                                                                                                                                                                      <  1:69236/78‑1 (MQ=255)
                                                                                                                                                                      gAGGCTGGTCTGCGAACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGAGATGTATCACTTCTCTCCCACCGAAACTTCCCGGCAAAACTTGCTGCGTGAAAACGTTGCGGATAGCCGAATCTTCATTACCGGTAATACAGTCATTGATGCACTGTTATGGGTGCGTGACCAGGTGATGAGCAGCGACAAGCTGCg                                           >  1:420028/1‑224 (MQ=255)
                                                                                                                                                                          cTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGCAAAACTTGCTGCGTGAAAACGTTGCGGATAGCCGAATCTTCATTACCGGTAATACAGTCATTGATGCACTGTTATGGGTGCg                                                                         <  1:239376/190‑1 (MQ=255)
                                                                                                                                                                             ntCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGCAAAACTTGCTGCGTGAAAACGTTGCGGATAGCCGAATCTCCATTACCGGTAATACAGTCATTGATGCACTGTTATGGGTGCGTGACCAGGTGATGAGCAGCg                                                     >  1:313995/2‑207 (MQ=255)
                                                                                                                                                                             gTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGCAAAACTtgctg                                                                                                                                                <  1:64534/116‑1 (MQ=255)
                                                                                                                                                                                         gCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTATATTGACCGGGCATCTGGCGATGTATCCCTTCTCTCCAACCGACACTTCCCGGCAAAACTTGCTGCGTGACAACGTTGCGGATAGCCGAATCTTCATTACCGGTAATACAGTCATTGATGCACTGTTATGGGTGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGAACCCGATa  >  1:382765/1‑246 (MQ=255)
                                                                                                                                                                                                                     ggCTAACCGTATATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGCAAAACTTGCTGCGTGAAAACGTTGCGGATAGCCGAATCTTCATTACCGTTAATACAg                                                                                                  <  1:389267/122‑1 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                              
GCAGCCAGGACAGGGCCTGACAGAGATAACCTGTCGGATTCTGGAAGGGCTAAAACCTATTCTTGCCGAGTTCAAACCAGACGTCGTGCTGGTTCACGGCGATACGACGACGACGCTGGCAACCAGCCTGGCGGCGTTTTATCAGCGTATTCCTGTTGGTCACGTTGAGGCTGGTCTGCGCACGGGCGATCTCTATTCGCCGTGGCCGGAAGAGGCTAACCGTACATTGACCGGGCATCTGGCGATGTATCACTTCTCTCCAACCGAAACTTCCCGGCAAAACTTGCTGCGTGAAAACGTTGCGGATAGCCGAATCTTCATTACCGGTAATACAGTCATTGATGCACTGTTATGGGTGCGTGACCAGGTGATGAGCAGCGACAAGCTGCGTTCAGAACTGGCGGCAAATTACCCGTTTATCGACCCCGATA  >  NC_000913/3970315‑3970745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑