| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 462,097 | C→T | S61S (AGC→AGT) | ppiD → | periplasmic folding chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 462,097 | 0 | C | T | 93.8% | 32.6 / ‑3.3 | 16 | S61S (AGC→AGT) | ppiD | periplasmic folding chaperone |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (10/5); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01 | |||||||||||
GTTGCGTGAGGTACACAGTCATCTACAGCGGAGTGTTGTTACACCATGATGGACAGCTTACGCACGGCTGCAAACAGTCTCGTGCTCAAGATTATTTTCGGTATCATTATCGTGTCGTTCATATTGACCGGCGTGAGTGGTTACCTGATTGGCGGAGGCAATAACTACGCCGCAAAAGTGAATGACCAGGAAATCAGCCGTGGGCAATTCGAGAACGCCTTCAACAGCGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCG > NC_000913/461870‑462324 | gTTGCGTGAGGTACACAGTCATCTACAGCGGAGTGTTGTTACACCATGATGGACAGCTTACGCACGGCTGCAAACAGTCTCGTGCTCAAGATTATTTTCGGTATCATTATCGTGTCGTTCATATTGACCGGCGTGAGTGGTTACCTGATTGGCGGAGGCAATAACTACGCCGCAAAAGTGAATGACCAGGAAATCAGCCGTGGGCAATTCGAGAACGCCTTCAACAGTGAGCGTAATCGCATGCAGCAAc < 1:248462/250‑1 (MQ=255) cGCACGCCTGCAAACAGTCTCGTGCTCAAGATTATTTTCGGTATCATTATCGTGTCGTTCATATTGACCGGCGTGAGTGGTTACCTGATTGGCGGAGGCAATAACTACGCCGCAAAAGTGAATGACCAGGAAATCAGCCGTGGGCAATTCGAGAACGCCTTCAACAGTGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTc > 1:140197/1‑250 (MQ=255) naaaCAGTCTCGTGCTCAAGATTATTTTCGGTATCATTATCGTGTCGTTCATATTGACCGGCGTGAGTGGTTACCTGATTGGCGGAGGCAATAACTACGCCGCAAAAGTGAATGACCAGGAAATCAGCCGTGGGCAATTCGAGAACGCCTTCAACAGTGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTcc > 1:306458/2‑200 (MQ=255) gCTCAAGATTATTTTCGGTATCATTATCGTGTCGTTCATATTGACCGGCGTGAGTGGTTACCTGATTGGCGGAGGCAATAAATACGCCGCAAAAGTGAATGACCAGGCAATAAGCCGTGGGCAATTCGAGAACGCCTTCAACAGTGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTc > 1:338268/1‑245 (MQ=255) nnnnnnnnnnaCNNNNNTGANTGGTTACCTGATTGGCGGAGGCAATAACTACGCCGCAAAAGTGAATGACCAGGAAATCAGCCGTGGGCAATTCGAGAACGCCTTCAACAGTGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTcc < 2:306458/144‑1 (MQ=255) gTGAGTGGTTACCTGATTGGCGGAGGCAATAACTACGCCGCAAAAGTGAATGACCAGGAAATCAGCCGTGGGCAATTCGAGAACGCCTTCAACAGTGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCtat > 1:25984/1‑162 (MQ=255) gCAATAACTACGCCGCAAAAGTGAATNACCAGGNNATNAGCCGTGGGCAATTCGAGANCGNNNTNNNCNGTGAGNGTNNTCGCATGCANCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCTATATGCANACCCTGCGTNNACAGGTGCTGAATCGTCTGATNGACNNGNNGNTGCNggn > 2:113908/1‑192 (MQ=255) aCTACGCCGCAAAAGTGAATGACCAGTAAATCAGCCGTGGGCAATTCGAGAACGCCTTCAACAGCGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTcgcg < 1:420197/251‑1 (MQ=255) cgccgcAAAAGTGAATGACCAGGAAATCAGCCGTGGGCAATTCGAGAACGCCTTCAACAGTGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGAccc > 1:13386/1‑251 (MQ=255) gTGAATGACCAGGAAATCAGCCGTGGGCAATTCGAGAACGCCTTCAACAGTGAGCGTAATCGCAGGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCa < 1:237634/134‑1 (MQ=255) aCCAGGAAATCAGCCGTGGGCAATTCGAGAACGCCTTAAACagtgag > 1:225889/1‑47 (MQ=255) ggAAATCAGCCGTGGGCAATTCGAGAAAGCCTTCAACAGTGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGc > 1:428739/1‑250 (MQ=255) ggCAATTCGAGAACGCCTTCAACAGTGAGCGTAATCGCATGCAGCAACCGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGACGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACCGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGc > 1:446883/1‑251 (MQ=255) gCAATTCGNNACNNCNTNCAACANTGAGCGTAATCGCATGCAGCAACAGCTggg > 2:342657/1‑54 (MQ=255) gCAATTCGAGAACGCCTGCAACAGTGAGCGTAATCGCATGCAGCAACAGCTggg < 1:342657/54‑1 (MQ=255) cAATTCGAGAACGCCTTCAACAGTGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCTGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCg > 1:504101/1‑251 (MQ=255) cAACAGTGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATcgacga < 1:430844/117‑1 (MQ=255) | GTTGCGTGAGGTACACAGTCATCTACAGCGGAGTGTTGTTACACCATGATGGACAGCTTACGCACGGCTGCAAACAGTCTCGTGCTCAAGATTATTTTCGGTATCATTATCGTGTCGTTCATATTGACCGGCGTGAGTGGTTACCTGATTGGCGGAGGCAATAACTACGCCGCAAAAGTGAATGACCAGGAAATCAGCCGTGGGCAATTCGAGAACGCCTTCAACAGCGAGCGTAATCGCATGCAGCAACAGCTGGGCGATCAATACTCCGAGCTGGCAGCGAACGAAGGCTATATGAAAACCCTGCGTCAACAGGTGCTGAATCGTCTGATCGACGAGGCGCTGCTGGATCAGTACGCACGTGAGCTGAAACTGGGTATCAGCGATGAGCAGGTTAAACAGGCGATTTTCGCGACCCCAGCCTTCCAGGTTGACGGCAAATTTGATAACAGCCG > NC_000913/461870‑462324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |