Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,640,418	IS30 (–) +2 bp	coding (17‑18/141 nt)	yjjY →	uncharacterized protein YjjY

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 271761	NA (NA)	19 (1.000)	18/272	0.1	100%	noncoding (1221/1221 nt)	IS30	repeat region
	?	NC_000913	= 4640419	0 (0.000)					coding (18/141 nt)	yjjY	uncharacterized protein YjjY
*	?	NC_000913	= 4508679	NA (NA)	19 (0.990)	15/276	0.1	100%	noncoding (1/1221 nt)	IS30	repeat region
	?	NC_000913	4640418 =	0 (0.000)					coding (17/141 nt)	yjjY	uncharacterized protein YjjY

CAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAa‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/271520‑271761 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGCATGCATCAGGCAGGTCAGGGACTTTTGTACTTCCTGTTTCGATTTAGTTGGCAATTTAGGTAGCAAACATGCAGACCCCGCACATTCTTATCGTTGAAGACGAGTTGGTAACACGCAACACGTTGAAAAGTATTTTCGAAGCGGAAGGCTATGATGTTTTCGAAGCGACAGATGGC  <  NC_000913/4640419‑4640199                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 CAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTAC                                                                                                                                                                                                                      <  1:437853/251‑1                                                                                    GGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGACAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAG                                                                                                                                                                                                                 >  1:88181/1‑173                                                                                           CTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAG                                                                                                                                                                                                                 <  1:460644/166‑1                                                                                                                          ACTCAACATGAACTAGATCTGGTTTGTGCTCAGCTAAACAACAGTCCGAGAAAGACACTGAAGTTCAAAACACCGAATGAGATAATTGAAAGGGGTATTGCATTGACAGATTGAATCTACAATTACGTACTTTAGT                                                                                                                                                                                                                <  1:75555/136‑1                                                                                                                              AACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGCCCGAGAAAGACACTGAAGTTCAAAACCCCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAAATTGACTCTACAATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGCATGCATCAGGCAGGTCAGGGACTTTTGTACTTCCTGTTTCGATTTAGTTGGCAATTTAGGAAGCAAACATGCAGACCCCGCACATTCTT                                                                                         >  1:80828/1‑251                                                                                                                                           TCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACG                                                                                                                                                                                                                         <  1:34456/110‑1                                                                                                                                            CTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGCTTGCATCAGGCAGGTCAGGGAC                                                                                                                                                            >  1:488491/1‑170                                                                                                                                               GTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGCATGCATCAGGCAGGTCAGGGACTTTTGTACTTCCTGTTTCGATTTAGTTGGCAATTTAGGTAGCAAACATGCAGACCCCGCACATTCT                                                                                          >  1:469379/1‑233                                                                                                                                                       TCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATACTTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGCATGCATCAGGATGGTCAGGGACTTTTGTACTTCCTGTTTCGATTTAGTTGGC                                                                                                                              >  1:485570/1‑189                                                                                                                                                        CAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGAGAAGTGAAAGGGGTTTTGCATTGACATATTGAATCTACAAGTACGTACTTTATTCATGTTACGCCGATCATTTTAATTTGCAGCATGCATCAGGCAGGTCAGGGACTTTTGTACTTCCTGTTTC                                                                                                                                          <  1:336808/176‑1                                                                                                                                                                 AACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGCATGCATCAGGCAGGTCAGGGACTTTTGTA                                                                                                                                                     >  1:144552/1‑156                                                                                                                                                                   CAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGCATGCATCAGGCAGGTCAGGGACTTTTG                                                                                                                                                       >  1:400633/1‑152                                                                                                                                                                               GACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGCATGCATCAGGCAGGTCAGGGACTTTTGTACTTCCTGTTTCGATGTAGTTGGCAATTTAGGTAGCAAACATGCAGACCCCGCACATTCTTATCGTTGAAGACGAGTTGGTAACACGCAACACGTTn                                                     <  1:93735/238‑2                                                                                                                                                                                 CACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCATCATGCATCAGGCANG                                                                                                                                                                    <  1:395272/125‑1                                                                                                                                                                                          aCAAAACACCGAAAGAGATAAGTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGAAAGCAGCAGGCAGGTCATGGACTATGGTACTACCTGATTCGATGTAGGAGGCAATTTAGGTAGCAAACATGCAGACCCCGCACAGTCTTATCGTTGAAGACGAGTTGGTAACACGCAACACGTTGAAAAGTATTTTCGAAGCGGAAGGC                             <  1:508678/250‑1                                                                                                                                                                                           CAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTACGCCGATCATNNTAATTTnn                                                                                                                                                                                     >  2:13320/1‑96                                                                                                                                                                                                      AAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGCATGCATCAGGCA                                                                                                                                                                      >  1:152754/1‑102                                                                                                                                                                                                                    AGGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTAC                                                                                                                                                                                                        >  1:335727/1‑54                                                                                                                                                                                                                     GGGGTGTTGCATTGACAGATTGAATCTACAATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGCATGCATCAGGCAGGTCAGGGACTTTTGTACTTCCTGTTTCGATTTAGTTGGCAATTTAGGTAGCAAACATGCAGACCCCGCACATTCTTATCGTTGAAGACGAGTTGGTAACACGCAACACGTTGAAAAGTATTTTCGAAGCGGAAGGCTATGATGTTTTCGAAGCGACAGATGGC  <  1:174558/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 CAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCTTGGCAGCGGGGAACAAATGAGAATACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATACTCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGATAATTGAAAGGGGTGTTGCATTGACAGATTGAATCTACAa‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/271520‑271761 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caATTACGTACTTTAGTCATGTTACGCCGATCATGTTAATTTGCAGCATGCATCAGGCAGGTCAGGGACTTTTGTACTTCCTGTTTCGATTTAGTTGGCAATTTAGGTAGCAAACATGCAGACCCCGCACATTCTTATCGTTGAAGACGAGTTGGTAACACGCAACACGTTGAAAAGTATTTTCGAAGCGGAAGGCTATGATGTTTTCGAAGCGACAGATGGC  <  NC_000913/4640419‑4640199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.