| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 313,543 | G→A | intergenic (‑301/‑1748) | ykgR ← / → insE1 | putative membrane protein YkgR/IS3 element protein InsE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 313,543 | 0 | G | A | 95.7% | 58.1 / ‑3.5 | 23 | intergenic (‑301/‑1748) | ykgR/insE1 | putative membrane protein YkgR/IS3 element protein InsE |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (12/10); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01 | |||||||||||
ACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGGAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTTATCCGGAATG > NC_000913/313298‑313785 | aCGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTg > 1:155228/1‑251 (MQ=255) aTAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTac < 1:14512/251‑1 (MQ=255) aGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGCGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGGAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTacac > 1:201417/1‑251 (MQ=255) tttATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGAGCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTAGACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCTGATTCATACTGGATTACACTGCAGGCACGCCTTATga < 1:217335/247‑1 (MQ=255) ccTGAAGTCAGTTCCGNGNATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGNGGAGCATATCNCATNNCATCNTNANGTCGTATCTCCTGGGGTGTTATACAAGATNTCGTTGTTGGTGACCGGGGAGAGNNATNNNGTNNTATTa < 2:372895/162‑1 (MQ=255) ccTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTa > 1:372895/1‑162 (MQ=255) ggAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCAc < 1:388360/191‑1 (MQ=255) gCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGCATCTCCTGGGGTGTTATACAAGAAATTGTTGTTGGCGACCTGGGAGAGAACTTGAGTTCTATTAAACCGCCAACTATGCCGGCTACATACTGGATTACACTGCAAGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGaa > 1:286495/1‑211 (MQ=255) ccGGGCGTTGATTGGGGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCATGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATGGATTTGATAGTTTTNc < 1:392304/251‑1 (MQ=255) gTTGATTGGAGCTGAGGAGCATATCGCATCTAATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTa > 1:429981/1‑142 (MQ=255) gATTGGTGCTGAGGAGCATATCGAATCTCATAATAATGTCGTATCTCCTGGGGCGTTATCCAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTCCACCGTCAACTTTGCCGGATACATACTGGATTACACTGCAg > 1:216388/1‑147 (MQ=255) ttGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTAGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGGTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCAttgatttg < 1:496502/230‑1 (MQ=255) gCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGTGTTAATTATCTGAAAGAATTTGTGAGTCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCAtt < 1:292841/251‑1 (MQ=255) gtTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTg > 1:30079/1‑123 (MQ=255) gtTATACAAGATATCGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTtgt > 1:438752/1‑150 (MQ=255) cttngttgGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACNNTNNNGNagacatac < 2:361478/62‑1 (MQ=255) cGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGatacatac > 1:361478/1‑64 (MQ=255) cGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCAt > 1:159066/1‑250 (MQ=255) cGTTGTTGGTGACCTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCGGAAAGAATTTGTGATGCTGTAGTGGTTACTCATGGATTTGATAGTTGGAGTCTGGGGAGGAGAATATATATTTATTCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATa < 1:277662/251‑1 (MQ=255) gNNCTGGGANNGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTg < 2:30079/99‑1 (MQ=255) ncNGGNAGAGANATTGNGTTCTATTAAACCGTNAACTATTCCGGATACATACNNGNTTACANTGCAg < 2:216388/66‑1 (MQ=255) cTGGGAGAGAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCAt > 1:401272/1‑237 (MQ=255) nagagAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGcc > 1:95708/2‑218 (MQ=255) nagagAAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTTATCCGGAATg > 1:61661/2‑248 (MQ=255) | ACGAGTTTGGTCAACCAATTTTTTGACATGTATCACAAATTTGAATAGATGTATTACATCAACTATCTTTTATTGCACCAACGTCATTGATATATGTCGCCTGAAGTCAGTTCCGGGAATGAGTCTGATCTCAAGACTGGCCCAGTCCGGGCGTTGATTGGTGCTGAGGAGCATATCGCATCTCATCATAATGTCGTATCTCCTGGGGTGTTATACAAGATATCGTTGTTGGTGACCTGGGAGAGGAATTGAGTTCTATTAAACCGTCAACTATGCCGGATACATACTGGATTACACTGCAGGCACGCCTTATGAGAGAACGTGCCGCAGTGACGGGTTAATTATCTGAAAGAATTTGTGAGGCTGTATCGGTTACTCATTGATTTGATAGTTTTACTCTCGGGAGAATAATAGATATTTAATCCATTAACGGAAACCAGCCAGTTCCTTTCGATGCCTGAATTTGATCCCATAGTTTATCCGGAATG > NC_000913/313298‑313785 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |