Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,540,703	G→A	E99E (GAG→GAA)	feoB →	Fe(2(+)) transporter FeoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,540,703	0	G	A	100.0%	31.5 / NA	12	E99E (GAG→GAA)	feoB	Fe(2(+)) transporter FeoB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/8);  total (4/8)

GGTAACTGGGCTGGCGTTACCGTCGAACGTAAAGAAGGGCAATTCTCCACCACCGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGC  >  NC_000913/3540497‑3540901                                                                                                                                                                                                               |                                                                                                                                                                                                       ggTAACTGGGCTGGCGTTACCGTCGAACGTAAAGAAGGGCAATTCGCCACCACCGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAACGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTcc                                                                                                                                                            <  1:329651/251‑1 (MQ=255)                  tACCGTCGAACGTAAAGAAGGGCAATTCTCCACCACCGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGATTGGTGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAACGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTg                                                                                                                                            <  1:199370/250‑1 (MQ=255)                                               ccaccaCCGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAACGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGc                                                                                                                           <  1:265284/238‑1 (MQ=255)                                                   caccGATCATCAGGGCACGCTTGGGGACCTGCCCGGCACCTATTCGCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAAGCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATGAACGATGTGCAGTCGTCGAACCTTGAACCTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGc                                                                                                                                                                 <  1:460474/196‑1 (MQ=255)                                                               ggTCANNCTNNNGGNNCTGCCCGGCACCTATTCTCTGACNACCATCTCATCGCAGACCTCGCTCGATGANCNANTCGCNNGTCNCTACATTTTGNGTGGCGACGCAGACCTGCTGATTAAAGTGGTGGATGCGTCTAACCTTGNANGTAACCTGTACCTGACGCTACAActgct                                                                                                                                                                           >  2:363709/1‑174 (MQ=255)                                                               ggTCACGCTGGGGGACCTGCCCGGCACCTATTCTCTGACCTCCATCGCATCGCAGACCTCGCGCGATGAGCAAATCGCCTGTCACTACATTGGGAGTGGCGACGCCGACCTGCTGATTAACGTGGTTGATGCGGCTAGCCTTGAACGTAACCTTTACCTGACGCTACAActgct                                                                                                                                                                           <  1:363709/174‑1 (MQ=255)                                                                        ggtggACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTAGCTAGATGAGCAAATCGCCCGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTCAACGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGAc                                                                                                                                >  1:446086/1‑208 (MQ=255)                                                                                       naCCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAACGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAg                                                                                                                    >  1:317624/2‑205 (MQ=255)                                                                                                                                                  nntANNNNNTGANTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAACGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAg                                                                                                                    <  2:317624/144‑1 (MQ=255)                                                                                                                                                            gAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAACGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGc  <  1:236615/250‑1 (MQ=255)                                                                                                                                                                 gCNACNCCGACCTGCTGAGGANCGTTTTGGATGCGTCTAACCTTGAACGTAACCTGTACCTGACGCNNCNNNTNCTGGAACt                                                                                                                                                                     <  2:351962/82‑1 (MQ=255)                                                                                                                                                                                                         ccTTGAACGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCg                                                                                                                                  >  1:442532/1‑77 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                       GGTAACTGGGCTGGCGTTACCGTCGAACGTAAAGAAGGGCAATTCTCCACCACCGATCATCAGGTCACGCTGGTGGACCTGCCCGGCACCTATTCTCTGACCACCATCTCATCGCAGACCTCGCTCGATGAGCAAATCGCCTGTCACTACATTTTGAGTGGCGACGCCGACCTGCTGATTAACGTGGTGGATGCGTCTAACCTTGAGCGTAACCTGTACCTGACGCTACAACTGCTGGAACTCGGCATTCCCTGCATTGTGGCACTGAACATGCTCGACATTGCCGAGAAGCAAAATATTCGTATTGAAATTGATGCTCTGTCGGCGCGTCTGGGCTGTCCGGTGATCCCGCTGGTTTCAACCCGTGGTCGCGGTATTGAAGCGCTCAAGCTGGCGATTGATCGC  >  NC_000913/3540497‑3540901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑