Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,357,2020CT100.0% 42.9 / NA 17intergenic (‑92/+221)sapA/ymjAputative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/10);  total (7/10)

GCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAAC  >  NC_000913/1356956‑1357392
                                                                                                                                                                                                                                                      |                                                                                                                                                                                              
gCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTTCTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTgtgt                                                                                                                                                                                            <  1:266193/251‑1 (MQ=255)
                              ccGCTGACGCAATAGACAACACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCAt                                                                                                                                                              >  1:205860/1‑251 (MQ=255)
                                   gatgCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTTAACAGGTGACGTCGCCATCTg                                                                                                                                                           <  1:112041/246‑1 (MQ=255)
                                            gACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCATGACCAATCACAAGTCCAGCAATCACCAAAAGAGACGATAATAACTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTTATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCg                                                                                                                                                >  1:491606/1‑251 (MQ=255)
                                                                  tCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGTGCTGTGTGGTTTGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCg                                                                                                                                                <  1:204359/229‑1 (MQ=255)
                                                                          cGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAAACTATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTg                                                                                                                                                           <  1:501864/210‑1 (MQ=255)
                                                                              ggAGATTCAGGCGCGGCGATTGCCTGACCACTCACACTTCCAGCAATCCACACTCGCGACGATAATACCTCGCGCATAATATTACGCGATTTTATGTCAAAAGGCTATCTTACTCATTTTTCATGACATTTGCCATTTCCGTTTGTGTTCAGGGGTCGAAGTGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTTAACATTCGTACCAGTGATTTCTCTataactataactaac                                                                                                                 >  1:243747/1‑245 (MQ=255)
                                                                                               gATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGCTTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGc                                                                                             >  1:15228/1‑251 (MQ=255)
                                                                                                       gACCACTCACAAGTCCAGCAAGCAACAAAAGAGACGATAATACCTTGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATTACATTTGCCATTACCGTTTGTGTTCAGGGGTCGGAATGAGGGCTGTGGGGTTGGGCGGTGAACAGTGGACGTCGCCAGCTGTTCAACATGTGTGCCCGTGATGTCTCTATAACTATAACTCACAGaacaac                                                                                                         <  1:333752/231‑1 (MQ=255)
                                                                                                         ccACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATTCCTGGCACATAATATTAAGTGATGTTATGTAAAGAGGCTATCTTACTAATATTGAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGCCGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCaga                                                                                          <  1:29239/244‑1 (MQ=255)
                                                                                                                caAGTCTAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATAACCTTTTGTGTGCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGGGACGTCGCCATCTGTGCAACATTCGGACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTg                                                                            <  1:502113/251‑1 (MQ=255)
                                                                                                                               ccAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTcac                                                                                                                 >  1:339661/1‑199 (MQ=255)
                                                                                                                                                     gcgcATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCAttn                                       <  1:130448/251‑2 (MQ=255)
                                                                                                                                                                                    ggCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATATCTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCg        <  1:84287/251‑1 (MQ=255)
                                                                                                                                                                                      cTATCGTACTAATATTTAATGACATTTGCCATTACCGTTAGTGTTCAGGGGTGGATATGAGGGATGTGTGGTTGGCAGTTGCACAGGTGa                                                                                                                                                                       >  1:341630/1‑90 (MQ=255)
                                                                                                                                                                                                                  ccATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGcc                                                                                                                                                                >  1:378626/1‑69 (MQ=255)
                                                                                                                                                                                                                                                 tgggCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACaan  <  1:47455/195‑2 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                              
GCTTTGGATGGGTTAAAGGTGTTGACTTGCCCGCTGACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAAC  >  NC_000913/1356956‑1357392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑