| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,823,583 | G→A | G395E (GGG→GAG) | spoT → | bifunctional (p)ppGpp synthase/hydrolase SpoT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,823,583 | 0 | G | A | 95.5% | 57.2 / ‑5.8 | 22 | G395E (GGG→GAG) | spoT | bifunctional (p)ppGpp synthase/hydrolase SpoT |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (10/11); minor base T (1/0); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
GAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCG > NC_000913/3823354‑3823791 | gAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGctgcctgc < 1:226466/251‑1 (MQ=255) gTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGTTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGTCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGTATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCgg < 1:345726/251‑1 (MQ=255) gtgtTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAAGCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGc < 1:151946/198‑1 (MQ=255) gCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTg > 1:23062/1‑205 (MQ=255) naCTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTGc > 1:122103/2‑200 (MQ=255) gggCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGTATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAgn < 1:298830/183‑2 (MQ=255) aTCCGCGCCCAGCGCGGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTGACGTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCg < 1:239049/166‑1 (MQ=255) cccAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGATCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGGGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATCCCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTAcc > 1:191513/1‑251 (MQ=255) cTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCCCACAGGAAGAGAGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTc > 1:332432/1‑251 (MQ=255) agagCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGc > 1:18761/1‑130 (MQ=255) gagCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCAcg > 1:160419/1‑174 (MQ=255) gagCGCCGGTAGGTCGTTTGAATTTATCGAGAGCGTGAAATCCGATCCCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGTCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAAcc < 1:451424/226‑1 (MQ=255) ttcagccgtgtgctcttccaatctCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACTTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCAGGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTCCCAGCGGTCAACCCGTTGAAATCATTACCGCTCCGGGCGCt < 1:25284/227‑1 (MQ=255) cGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCNCACCGGAAGANCGCNNTGTCNANCNGCCGGCCGGTGCAACGCCCGTCGACTTCGNTTATGCAGTGCATTCCGATATCGNNCANNNCTNNGTggg < 2:381522/141‑1 (MQ=255) cGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTGCAACGNNCGTCg > 2:25284/1‑95 (MQ=255) cGAGAGCGTTAAATCCGATATCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGTGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCACCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCCCCCGAATGCCGCTTGGCTGAACt > 1:412090/1‑251 (MQ=255) cGAGAGCGTTAAATCAGATCTCTTCCCGGATGAGATTTACGTTTTCACACAGGCAGAGCGCATTGTCGAGCTGACTGACGGTGCAACGCCCGTCGACTTCGCTAATGCAGTGCATACCGATATCGGTCATGACGGCGTggg > 1:381522/1‑141 (MQ=255) gagnGNNTNNAATNCGATCGCTTCCCGGATGAGATNNACGTTTTCANACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGNTTATGCAGTNNATNCCGAGNTNNNTNNNGCNTGCGTGGGCGCACGCGTTGNCCNNCAGCCTNACCCGCTGTCGCAGCCGCTTACCAGc < 2:120324/189‑1 (MQ=255) gagaGCGTTAAATACGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGc > 1:120324/1‑189 (MQ=255) cGTTAAATCCGAGCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGGTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCg < 1:428996/251‑1 (MQ=255) cctCCCGGATGAGATTTACGGTTTCACACCGGAAGAGCGCACTGACGAGCTGCCTGCCGGTGCAACGCCCGTCGACTGCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCg < 1:216974/242‑1 (MQ=255) ccGGATGAGATTTACGTTTTCACACCGGAAGAGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGtt > 1:374241/1‑177 (MQ=255) | GAGGTCCAGATCCGTACCGAAGATATGGACCAGATGGCGGAGATGGGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAGCGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTGCCTGCCGGTGCAACGCCCGTCGACTTCGCTTATGCAGTGCATACCGATATCGGTCATGCCTGCGTGGGCGCACGCGTTGACCGCCAGCCTTACCCGCTGTCGCAGCCGCTTACCAGCGGTCAAACCGTTGAAATCATTACCGCTCCGGGCGCTCGCCCGAATGCCGCTTGGCTGAACTTTGTCGTTAGCTCG > NC_000913/3823354‑3823791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |