Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,148,391	C→T	V165V (GTC→GTT)	gpr →	L‑glyceraldehyde 3‑phosphate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,148,391	0	C	T	100.0%	31.3 / NA	12	V165V (GTC→GTT)	gpr	L‑glyceraldehyde 3‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/5);  total (7/5)

ACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGC‑CAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCAC  >  NC_000913/3148182‑3148623                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          aCCAAGGTTGGTTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATTCCTGCTCGCCAGCCTCGACGAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTGACTCCCTGCGGGTCGATTAAAATCCGCCGATGGAAGAAGCCGCCTCTGCGCGGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTTGGGATCTCCTCTTGCTCGC‑CAGAGCGGACGCAAAAAATgg                                                                                                                                                                                                  <  1:203951/250‑1 (MQ=255)                                            ncGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTTGGGATCTCCTCTTACTCGC‑CAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATtcatca                                                                                                                                                       >  1:146656/2‑250 (MQ=255)                                                                        ccTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTGGATATCTTTTACTCTCATCTCTTCGATGAAAATACGCGGAGGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGGACAAAGCGGTAAGGCGCTGTATGTTGGGATCTCCTCTTACTCGC‑CAGAGCGGAc                                                                                                                                                                                                             <  1:240089/168‑1 (MQ=255)                                                                                                    cTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGGAAATACGCCGGTGGAAGAAACCGCAACTGAGATGTCTCATGCCGTACAACGCGGTAACACGCTGTATGTTGGGATCTCCTCTTACTCGCACA‑AGAGGACGAAAACAATGGTCGAGTTGCTACGCGAGAGGAACATTCCGCTGTTCAtt                                                                                                                                                            >  1:343209/1‑189 (MQ=255)                                                                                                                                           gAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGATCATGCGGTACAAAGCGGTAAGGCGCTGTATGTTGGGATCTCCTCTTACTCGC‑CAGAGCGGACGCAACAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAAc                                                                                                                                                     >  1:462905/1‑157 (MQ=255)                                                                                                                                              aaTACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTTGGGATCTCCTCTTACTCGC‑CAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTTTACTCCTCTggn                                                    <  1:298491/251‑2 (MQ=255)                                                                                                                                                     cGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTACGGCGCTGTATGTTGGGATCTCCTCTTACTCGA‑CAGAGCGGACGCAAAAAAt                                                                                                                                                                                                    >  1:335794/1‑100 (MQ=255)                                                                                                                                                              cagacgagtgctcctccaatcttGCGGTACAAAGCGGTAATGCTCTGTATGTTGGGATCTCCTCTTACTCGC‑CAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTTCAATTTACTGATCCGCTGGTTGGATAAAAGCGGCCTGCTTGATACCCGGCAAAATAACGGCGTGGGCTGTATTGCCTTTTCTCCTCTGGCTCAGGGATTGCTGAc                                     <  1:195981/228‑1 (MQ=255)                                                                                                                                                               naCCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGAGCTGTATGTTGGGATCTCCTCTTACTCGC‑CAGAGCGGACGCAAAAAATGGTCGAGTTGATGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACATTCGTACAATTTACTGAACCGCTGGGTGGATAAAAg                                                                                                                >  1:45948/2‑174 (MQ=255)                                                                                                                                                               aaCCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTTGGGATCTCCTCTTACTCGC‑CAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGACCg                                   <  1:136700/251‑1 (MQ=255)                                                                                                                                                                                cTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTTGGGATCTCCTCTTACTAGA‑CCGAGCGGACGCACAAAATGGTCGAGTTGCTGCGCCAGTGGACAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCctgct                                                                                                       >  1:504905/1‑166 (MQ=255)                                                                                                                                                                                                nggTAAGGCGCTGTATGTTGGGATCTCCTCTTACTCGC‑CAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCAc  >  1:125100/2‑251 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                                          ACCAAGGCTGGCTACGATATGTGGCCCGGCCCTTACGGCTCTGGCGGTTCACGTAAATACCTGCTCGCCAGCCTCGACCAAAGCCTGAAGCGTATGGGGCTTGAGTATGTCGATATCTTTTACTCTCATCGCGTCGATGAAAATACGCCGATGGAAGAAACCGCCTCTGCGCTGGCTCATGCGGTACAAAGCGGTAAGGCGCTGTATGTCGGGATCTCCTCTTACTCGC‑CAGAGCGGACGCAAAAAATGGTCGAGTTGCTGCGCGAGTGGAAAATTCCGCTGTTAATTCATCAACCTTCGTACAATTTACTGAACCGCTGGGTGGATAAAAGCGGCCTGCTGGATACCCTGCAAAATAACGGCGTGGGCTGTATTGCCTTTACTCCTCTGGCTCAGGGATTGCTGACCGGAAAATATCTCAACGGCATTCCGCAAGATTCAC  >  NC_000913/3148182‑3148623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑