Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,613,247	G→A	R279R (CGG→CGA)	yjjU →	putative patatin‑like phospholipase YjjU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,613,247	0	G	A	100.0%	50.8 / NA	19	R279R (CGG→CGA)	yjjU	putative patatin‑like phospholipase YjjU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/10);  total (9/10)

AGGGCGCTAAAACGTTGGTCGTCATTCGCACTGTGCCGTCACAAATGTACTACACGCCGCAGTGGTTCAAACGCATGGAACGCTGGCTGGGTGACAGTAGCCTGCAGCCGCTGGTCAATCTGGTGCAGCATCATGAAACCAGCTATCGTGACATTCAGCAATTTATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCCATTACATAGTATCGCGCTTGGCAGTCGGATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACC  >  NC_000913/4613003‑4613455                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                 aGGGCGCTAAAACGTTGGTCGTCATTCGCACTGTGCCGTCACAAATGTACTACACGCCGCAGTGGTTCAAACGCATGGAACGCTGGCTGGGTGACAGTAGCCTGCAGCCGCTGGTCAATCTGGTGCAGCATCATGAAACCAGCTATCGTGACATTCAGCAATTTATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCTATTACATAGTATCGCGCTTGGCAGTCGAATTcct                                                                                                                                                                                                            <  1:143511/251‑2 (MQ=255)                  ntcgtcATTCGCACTGTGCCGTCACAAATGTACTACACGCCGCAGTGGTTCAAACGAATGGAACGCTGGCTGGGTGACAGTAGCCTGCAGCCGCTGGTCAATCTGGTGCAGCATCATGAAACCAGCTATCGGGACATTCAGCAATTTATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCAATTACATAGTATCGCGCTTGGCAGTCGAATTCCGGCGCTGCGTGagagcgg                                                                                                                                                                                           >  1:63181/2‑245 (MQ=255)                    cgtcATTCGCACTGTGCCGTCACAACTGTACTACACGCCGCCGTGGTTCAAACGCATGGAACGCTGGCTGGGTGACAGTAGTCTGCAGCCGCTGGTCAATCAGGTGCAGCATCATGAAACCAGCTATCGTGACATTCAGCAATTTATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCCATTACATAGTATAGCGCTTGGCAGTCGAATTCCGGCGCTGCGTGCAGACtat                                                                                                                                                                                          >  1:473752/1‑250 (MQ=255)                                                                 gTTCAAACGCATGGAACGCTGGCTGGGTGACAGTAGCCTGCCGCCGCTGGTCACTCTGGTGCAGCATCATGACACCAGCTATCGTGACATTCAGCAATTTATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCCATTACATAGTATCGCGCTTGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGc                                                                                                                                             >  1:456753/1‑250 (MQ=255)                                                                 gTTCAAACGCATGGAACGCTGGCTGGGTGACAGTAGCCTGCAGCCGCTGGTCAATCTGGTGCAGCATCATGAAACCAGCTATCGTGACATTCAGCAATTTATAGAGAAACCTCCGGGCAAGCGGCGGATATTCGAAATTTATCCGCCGAAGCCATTACATAGTATCGCGCGTGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCa                                                                                                                                            <  1:501373/251‑1 (MQ=255)                                                                      aaCGCATGGAACGCTGGCTGGGTGACAGTAGACTGCAGCCGCTGGTCAATCTGGTGCAGCATCATGAAACCAGCTATCGTGACATTCAGCAATTTATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCCATTACATAGTATCGCGCTTGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTg                                                                                                                                               >  1:180351/1‑243 (MQ=255)                                                                                         gggTGACAGTAGCCTGCAGCCGCTGGTCAATCTGGTGCAGCATCATGAAACCAGCTATCGTGACATTCAGCAATTTATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCAATTACAGATTATCGCGCTTGGCAGTCGAATTCCGGCGCTGCGTGAATACTATAACCTTGGGCGTTTTTGCGGTCGTTAGTTCCTCGCCACGGTTGGCAGGCTATTAACTGAAAAAGcgcc                                                                                                                      <  1:497626/249‑1 (MQ=255)                                                                                                        gCAGCCGCTGGTCAATCTGGTGCAGCATCATGAAACCAGCTATCGTGACATTCAGCAATTTATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCCATTACATAGTATCGCTCTTGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTgg                                                                                                     <  1:256721/251‑1 (MQ=255)                                                                                                                   tCAATCTGGTGCAGCATCATGAAACCAGCTATCGTGACATTCAGCAATTTATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCCATTACATAGTATCGCGCTTGGCCGTCGAATNCCGNNNNNGc                                                                                                                                                                                                     >  2:316187/1‑144 (MQ=255)                                                                                                                                           ccAGCTATCGTGACATTCAGCAATTTATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCCATTACATAGTATCGCGCTTGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTGATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCg                                                                  <  1:205498/251‑1 (MQ=255)                                                                                                                                                       aCATTCAGCAATTGATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCCATTACATAGTATCGCGCTTGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATGTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTc                                                      <  1:468390/251‑1 (MQ=255)                                                                                                                                                                       tGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGACATTACATAGTCTCGAGCTTGGCAGACGAATTCAGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCATCGCCACGGTTGGCTAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGAt                                                                            >  1:489312/1‑213 (MQ=255)                                                                                                                                                                                                      gAAATTTATCCGCCGAAGCCATTACATAGTATCGCGCTTGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAACGTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAATCGCCGCTTACTCGCCATCTGGTGCCAGTTGTTACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGc                                         <  1:467401/217‑1 (MQ=255)                                                                                                                                                                                                            tATCCGCCGAAGCCATTACATAGTATCGCGCTTGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAACTTGGGTGTTTATGCGGTCTTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATCCGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGAcn  <  1:316187/250‑2 (MQ=255)                                                                                                                                                                                                                 nccgAAGCCATTACATAGTATCGCGCTTGGCAGTCGAATTctg                                                                                                                                                                                                            >  1:46071/2‑41 (MQ=255)                                                                                                                                                                                                                   cgAAGCCATTACATAGTATCGCGCTTGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGc                                                                                                                                                                        <  1:167213/77‑1 (MQ=255)                                                                                                                                                                                                                            tACATAGTATCGCGCTTGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGc                                                                                                                                         >  1:197920/1‑99 (MQ=255)                                                                                                                                                                                                                                          cTTGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAAATTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCCAGCTATTAACTGAAAAAGCGCCGCTTACCCGACATATGGTGCCAGTGGTGACGCCGGAATAGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTg                           >  1:207872/1‑195 (MQ=255)                                                                                                                                                                                                                                            tGGCAGTCGAATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCgn              <  1:150966/206‑2 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                 AGGGCGCTAAAACGTTGGTCGTCATTCGCACTGTGCCGTCACAAATGTACTACACGCCGCAGTGGTTCAAACGCATGGAACGCTGGCTGGGTGACAGTAGCCTGCAGCCGCTGGTCAATCTGGTGCAGCATCATGAAACCAGCTATCGTGACATTCAGCAATTTATTGAGAAACCACCGGGCAAGCTGCGGATATTCGAAATTTATCCGCCGAAGCCATTACATAGTATCGCGCTTGGCAGTCGGATTCCGGCGCTGCGTGAAGACTATAAACTTGGGCGTTTATGCGGTCGTTATTTCCTCGCCACGGTTGGCAAGCTATTAACTGAAAAAGCGCCGCTTACCCGCCATCTGGTGCCAGTGGTGACGCCGGAATCGATTGTCATTCCGCCTGCGCCAGTCGCCAACGATACGCTGGTTGCCGAAGTGAGCGACGCTCCGCAGGCGAACGACC  >  NC_000913/4613003‑4613455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑