| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,358,331 | C→T | G286D (GGC→GAC) | puuP ← | putrescine:H(+) symporter PuuP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,358,331 | 0 | C | T | 95.7% | 58.8 / NA | 23 | G286D (GGC→GAC) | puuP | putrescine:H(+) symporter PuuP |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (14/8); minor base G (0/1); total (14/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGACGCCGCGATAAAGATAACGCCACCGTAGACCGCCGTCAGGAAGATGGCTTTCGGGATCACGCGTGCGGCGTCCGGCGTCTCTTCCGAAAGCGTGGTCACCGCATCGAAACCGAGGAAGGAGAAACAGACAATCGTCGCC > NC_000913/1358128‑1358558 | ccGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCttt > 1:11332/1‑250 (MQ=255) aGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGACACACATTCTCACACCACATCACATACAGCCGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGCGCAGAGGACAATCGACTGGAAGAGCTTGTCGCCGa > 1:279114/1‑175 (MQ=255) aaaaCACATTGTCACGCCCCATCTCATACAGCAGACGCGACACGCTGTCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACATCGCAATTTCAGGCAGTGCGGCAACCGGATCTTTAAAGCGGCTGATAGCGGGAAATAACAGCTGCATAAAGAACGACTCCTCGATAAAGATAACGCCACCGTAGACCGCCGTCAGGAAg < 1:381892/250‑1 (MQ=255) gTCACGCCCCATCACCTACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGATCTTGTCGCCGACGTACAgcg > 1:388470/1‑125 (MQ=255) tcccATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTAGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGGGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGACGCCGCGATAAAGATAACGCCACCGTAGa > 1:367775/2‑221 (MQ=255) ccccATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTAGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGACGCCGCGATAAAGATAACACCACCGTAGa > 1:367762/1‑221 (MQ=255) gcgACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAANCGGNNNnnatnnnnnnnnnn > 2:309561/1‑144 (MQ=255) gcgACACGCGGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGAcgccn < 1:309561/177‑2 (MQ=255) cATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGTGCGTCATCCgg < 1:384727/112‑1 (MQ=255) tgtgAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAggn < 1:125138/96‑2 (MQ=255) aaGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAANTTCNGGCANTGNGGCATCCGGATCTGTNAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGACGCNNCNNNANAGATAACg < 2:357272/173‑1 (MQ=255) aaGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGAAGAGGAAAATAGACTGCAAGAGCTTGTCGCCGACGTACAGCGCCCTTTCAGGCAGTGCGGCATCAGGATCTTTAACCCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGACGCCGCGATCAAGATAACg > 1:357272/1‑173 (MQ=255) ccaggccagAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGGCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGTAAGANc < 1:394054/134‑1 (MQ=255) gccagAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGCCGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGACAGAACAGCTGCATAAAGAACGACGCCGCGATAAAGATAACGCCACCGTAGACCGCCGTCAGGAAGATGGCTTTCGGGATCACGCGTGcggcgtccggcg > 1:234740/1‑224 (MQ=255) ncagAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGACGCCGCGATAAAGATAACGCCACCGTAGACCGCCGTCAGGAAGATGGCTTTCGGGATCACGCGTGCGGCGTCCGGCGTCTCTTCCGAAAGCGTGGTCACCGCATc > 1:132147/2‑251 (MQ=255) gtTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGACGCCGCGATAAAGATAACGCCACCGTAGACCGCCGTCAGGAAGATGGCTTTCGGGATCACGCGTGCGGCGTCCGGCGTCTCTTCCGAAAGCGTGGTCACAGCATCGAAACCGaggaagg > 1:334964/1‑251 (MQ=255) caAACGTGGTGCAGAGGAAAATCGACGGGAATAGCTTGTCGCCGACGTACAGCGCAATGTCAGGCAGTGCGGCATCCGTATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGACGCCGCGATAAAGATAACgn < 1:48607/147‑2 (MQ=255) ntgCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGaaa > 1:100560/2‑98 (MQ=255) gtgCAGAGGAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGANNTCGGGan > 2:30800/1‑96 (MQ=255) gtacAGAGGAAAATCGACTTGAAGAGCTTGTCGCCGGCGTACAGCGCAATTTTAGGCAGTGCGGCCTCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAac < 1:30800/114‑1 (MQ=255) ggAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGCAAGAACAGCTGCATAACGAACGACGCCGCGATAAAGATAACGCCACCGTAGACCGCCGTTAGGAAGATGGCTTTCGGGATCACGCGTGCGGCGTCCGGCGTCTCTTCCGAAAGCGTGGTCACCGCATCGAAACCGAGGAAGGAGAACCAGACAATCGTCGcc > 1:499169/1‑251 (MQ=255) ggAAAATCGACTGGAAGAGCTTGTCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGACGCCGCGATAAAGATAACGCCACCGTAGa > 1:80092/1‑141 (MQ=255) cTGGAAGAGCTTGTCGCCGTCGTACAGCGCAATGTCAGGCAGTGCGGCATCCGTATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGACGCCGCGATAAAGAAAACGCCACCGTAGACCGCCGTCAGGAAGATGGCTTTCGGGATCACGCGTGcggcgtccggcgtc < 1:288035/181‑1 (MQ=255) | CCGACAACGCGACAATCCCGACCATAATGACGTTGAGTGCCGGAGTCCGCCATTTTGGGTGCACATAGCCAAACACGCGCTCCGGAAACACATTGTCACGCCCCATCACATACAGCAGACGCGACACGCTGGCATGTGAAGCCAGGCCAGAGGCTAACGTGTTCACAAACGTGGTGCAGAGGAAAATCGACTGGAAGAGCTTGCCGCCGACGTACAGCGCAATTTCAGGCAGTGCGGCATCCGGATCTTTAAAGCGGCTGATATCGGGAAAGAACAGCTGCATAAAGAACGACGCCGCGATAAAGATAACGCCACCGTAGACCGCCGTCAGGAAGATGGCTTTCGGGATCACGCGTGCGGCGTCCGGCGTCTCTTCCGAAAGCGTGGTCACCGCATCGAAACCGAGGAAGGAGAAACAGACAATCGTCGCC > NC_000913/1358128‑1358558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |