Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,038,660	C→T	G55S (GGC→AGC)	dsbC ←	protein disulfide isomerase DsbC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,038,660	0	C	T	100.0%	17.0 / NA	8	G55S (GGC→AGC)	dsbC	protein disulfide isomerase DsbC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/3);  total (5/3)

GGTAATATCAGTAAACACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGTTAGTCAGAACTGTCTTCATGCCAGCTACAGGCGCGGGCTGAATATCGCTGCTTTTGATGCCCATTTTGGCTAACGTTTGTTGAATTGCCGCGTCATCAGCCTGAGCAAAGCCTGAAAACGCCGCTAACAAAGTAAACAACATAAAACCTTTCTTCATAAATCTTCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACAT  >  NC_000913/3038472‑3038902                                                                                                                                                                                             |                                                                                                                                                                                                                                                   ggTAATATCAGTAAACACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCTGCTGTTAGTCAGAACTGTCTTCATGCCAGCTACa                                                                                                                                                                                                                  >  1:381255/1‑223 (MQ=255)                                       gcggcgCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGGGATGTACAACACGCTGCTGTTAGTCAGAACTGTCTTCATGCCAGCTACAGGCGCggga                                                                                                                                                                                                         <  1:64994/194‑2 (MQ=255)                                             cTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCAGCATCGGTGATGTACAACACGCTGCTGTTAGTCAGAACTGTCTTCATGcc                                                                                                                                                                                                                         <  1:185725/172‑1 (MQ=255)                                                                          ncATTCAACTGCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAATACGCTGCTGTTAGTCAGAACTGTCTTCATGCCAGCTACAGGCGCGGGCTGAATATCGCTGCTTTTGATGCCCATTTTGGc                                                                                                                                                                         >  1:150063/2‑191 (MQ=255)                                                                           cATTCAACTGCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCTGCTGTTAGCCAGACATGTCTTCATGCCAGc                                                                                                                                                                                                                      >  1:410800/1‑145 (MQ=255)                                                                                                                         cgttccACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCTGCTGTTAGTCAGAACTGTCTTCATGCCAGCTACAGGCGCGGGCTGAATATCTCTGCTTTTGATGCCCATTTTGGCTAACGTTTGTTGAATTGCCGCGTCATCAGCCTTAGCAAAGCCGGAAAACGCCTCTAACAAAGTAAACAACATAAAACCTTTCTTCATAAATc                                                                             <  1:324812/232‑1 (MQ=255)                                                                                                                                                                             gTGATGTACAACACGCTGGTGTTAGTCAGAACTGTCTTCATGCCAGCTACAGGCGCGGGCTGAATATCGCTGCTTTTGATGCCCATTTTGGCTAACGTTTGTTGAATTGCCGCGTCATCAGCCTGAGCAAAGCCTGAAAACGCCGCTAACAAAGTAAACAACATAAAACCTTTCTTCATAAATCTTCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAg                            >  1:137553/1‑233 (MQ=255)                                                                                                                                                                                     caacaCGCTGCTGTTAGTCAGAACTGTCTTCATGCCAGCTACAGGCGCGGGCTGCATATCGCTGCTTTTGATGCCCATTTTGGCTAACGTTTGTTGAATTGCCGCGTCATCAGCCTGAGCAAAGCCTGAAAACGCCGCTAACAAAGTAAACAACATAAAACCTTTCTTCATAAATCTTCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACAt  >  1:219890/1‑251 (MQ=255)                                                                                                                                                                                             |                                                                                                                                                                                                                                                   GGTAATATCAGTAAACACGGTGATGACGTGTTTTTCCTGCGGCGCTTTATAAACGATCATCTCTTTTTCAAGCGCATTCAACTGCTTTAACAGCATCTTATTGGTGACATTGACCGGAGCCGTGCCACTAACGTCATACATTGGCCCCTGAATGATATGTTTACCATCATCGGTGATGTACAACACGCCGCTGTTAGTCAGAACTGTCTTCATGCCAGCTACAGGCGCGGGCTGAATATCGCTGCTTTTGATGCCCATTTTGGCTAACGTTTGTTGAATTGCCGCGTCATCAGCCTGAGCAAAGCCTGAAAACGCCGCTAACAAAGTAAACAACATAAAACCTTTCTTCATAAATCTTCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACAT  >  NC_000913/3038472‑3038902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑