| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 364,850 | G→A | Q486* (CAG→TAG) | lacZ ← | beta‑galactosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 364,850 | 0 | G | A | 94.1% | 36.7 / ‑3.6 | 18 | Q486* (CAG→TAG) | lacZ | beta‑galactosidase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (8/8); total (9/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01 | |||||||||||
AAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAGCGCGGATCATCGGTCAGACGATTCATTGGC > NC_000913/364635‑365040 | aaaCGCCTGCCAGTATTTAGCGAAACCGCCAAGACGGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGATCGACAGATTTGATCCAGc < 1:3975/251‑1 (MQ=255) ggCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGAAGCGAAAGCCGTTTTTTGATGGACCATTTCGGCCCAGCCGGGCAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCCAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGATCGACAGATTTGATCCCGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGAcc > 1:432526/1‑251 (MQ=255) cGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGAt < 1:268302/251‑1 (MQ=255) cgcgTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGTCAAATAATATCGGTGGCCGTGGTGTCTGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGAtn < 1:104807/158‑2 (MQ=255) cGAAAGCCATTTTTTGATGGACCATTTCGGCACACCCGGGAAGGGCTGGTCTTCATCAACGCGCGCGCACATCGGGCAACTAATATCGGTGGCCGCGGTGTCGGCTCCGCCGCCTTCCTACTACACCGGGCGGGAAgg > 1:194503/1‑138 (MQ=255) ccATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTACACCTGGCGGGAATGATCGACAGATTTGATCCAGCGAGACAGCGCGTCGTGATTAGCGCCGTGGCCTGATGCATTCCCCAGCGAGCAGATGATCACTCTCGGGTTATTATGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCg < 1:504414/251‑1 (MQ=255) ggCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCCTTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGt > 1:201823/1‑249 (MQ=255) ccGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAAGATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGATCGACGGATGTGATCCAGCGATACAGCGCGTCGGGATTAGCGCCGTGGGCTGATTCAGTCCCCAGCTACCAGATGATCACACTCGGGTGGTTACGAGCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAgcgc < 1:472850/251‑1 (MQ=255) cGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAGcgcg > 1:115294/1‑251 (MQ=255) gggcTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCa > 1:390201/4‑198 (MQ=255) ggTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGCGGTCTCGGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGCTACAGCGCGTCGTGATTAGAGCCGCGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCCCCATTCGAGTTACGCGTTCGCTCCTCGCCGGTAGCCAGCGCGGATCATCg > 1:279710/1‑248 (MQ=255) tCATCCACGCGCGCGTACAGCGGGCAAATAATAACGGTGGCCGGGGTTTCGGCTCCGCCTCCTTCATACTACACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATAc < 1:496083/111‑1 (MQ=255) cgcgTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTCCACCGGGCGGGCAAGATCGACAGTTTTGATCCAGCGATACAGCGCGTCGTGCTTAGCGCCGTGGCATGATTCATTCCCCAGCGACCAAATGATCACACTCGGGTGATTACGATCGCTCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAGAGCGTATCATCGGTCAGACGCTTCATTGGc > 1:327990/1‑250 (MQ=255) aTCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCAt < 1:258661/200‑1 (MQ=255) tCGGGCAAATAATATCGGTGGCCGTGNTGTCGGNNCCNCCGCCTTCATACTACACCGNGCNNNANNNANAGACANATNNGATCCAGCGNTGCAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATNCTCTCACTCNNGTGATTACGCTCGCGCTGCACNATTNNCNNTNcgcngt > 2:108453/1‑189 (MQ=255) ncNGGNAAATANTATCNGTGGCCGTGGTGTCGNCTCCTCCGCCTTCATACTANNCNGGGGGNGAAgg < 2:194503/66‑1 (MQ=255) ggCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTACACCGGGCGGGAAGGATCGCCAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTcgcg > 1:427315/1‑180 (MQ=255) cTTCATACTACACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCTTGATTAGCCCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACTATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAgcgc < 1:465448/173‑1 (MQ=255) | AAACGCCTGCCAGTATTTAGCGAAACCGCCAAGACTGTTACCCATCGCGTGGGCGTATTCGCAAAGGATCAGCGGGCGCGTCTCTCCAGGTAGCGAAAGCCATTTTTTGATGGACCATTTCGGCACAGCCGGGAAGGGCTGGTCTTCATCCACGCGCGCGTACATCGGGCAAATAATATCGGTGGCCGTGGTGTCGGCTCCGCCGCCTTCATACTGCACCGGGCGGGAAGGATCGACAGATTTGATCCAGCGATACAGCGCGTCGTGATTAGCGCCGTGGCCTGATTCATTCCCCAGCGACCAGATGATCACACTCGGGTGATTACGATCGCGCTGCACCATTCGCGTTACGCGTTCGCTCATCGCCGGTAGCCAGCGCGGATCATCGGTCAGACGATTCATTGGC > NC_000913/364635‑365040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |