| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,236,745 | G→A | G69D (GGT→GAT) | ygjQ → | DUF218 domain‑containing protein YgjQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,236,745 | 0 | G | A | 94.1% | 35.1 / ‑3.6 | 17 | G69D (GGT→GAT) | ygjQ | DUF218 domain‑containing protein YgjQ |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (6/10); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01 | |||||||||||
CGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGT > NC_000913/3236548‑3236946 | cgcATTTGCCCGCCTTCTTCTCCGAATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATTGCGTAATTGTCAACGCCAGCAAACAGCTGACCTGGAGCGTTGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTTGGGGCGCGGCCGGGTAATCGCTACTTTACCCGACGTATTGCTACCGCAGCAGCGTTGTACCACGCCg > 1:151410/1‑251 (MQ=255) ccGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGATAATCGCTACTTTACCCGACGTATTGATAccn < 1:316724/221‑2 (MQ=255) tATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCCGGAGAGACCATACTAATCACAGATCGCGTAATGGTCAACGCCAGCACACAGCAGACCTGGAGCGATGTAAAAGCACTTCCCGAGCGGCCCGTGGGTTTATTGCCGCGGGCGCGGCCGGATCACCGCTAATTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGACGGAAAAGTGAAATGgctgctagatt > 1:207826/1‑246 (MQ=255) cGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGGGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGATAATCGCTACTGTGCCCGACGTAGTGATACCGCAGCAGCTTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACg < 1:411722/250‑1 (MQ=255) naTCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGCCAAAGCCGTTCCGGCGCGTAAAGTGGGTTTATTGCTGGGGGCGAGGCCGGATAATCGCTACTTTACCCGACGAATTGATACCGCAGCCGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCt > 1:57029/2‑250 (MQ=255) cGATCGCGTAATGGTCAACGCCAGNAAACAGCTGANCTGNNTCGANGNCNACGCCGTTCCGGCGCGTAACGTGGGTTTANTGCTGGGGGCGAGGCCGGATAAGNNCTNNNTTNNCCGAc < 2:365859/119‑1 (MQ=255) cGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCCCCGCCGTTCCGGCGCGTAACGTGGGATTCTTGCTGGGGGAGAGGCCGGATAATCGCTACTTTACCCGAc > 1:365859/1‑119 (MQ=255) cgcgTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTATCGTGGGTTTATTGCTGGGGGCGAGGCCGGATAATcn < 1:299504/101‑2 (MQ=255) cgcgTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGATAATCg > 2:299504/1‑101 (MQ=255) gcgTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGACAACGCCGGTCCGGCGAATAACGTGGGATTATTCCTGGGGGCGAGGCAGGATAATAGCTACTTTACCAGCCGTATTGATACCGCAGCAGCGTTGTACAACGCCGGAAAAGTGACATGGCAGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCaaa > 1:75103/1‑250 (MQ=255) cGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGATAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGc < 1:424430/163‑1 (MQ=255) aCAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACATGGGTTTATTGCTGGGGGCGAGGCCGGATAATCGCTACTTTACCCGACGTAGTGATACCGCAGCTGCTTTGTACCACGCCTGAAAAGTGAAATGGCTGTTGGTAAGTTGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTAt < 1:487183/192‑1 (MQ=255) cTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGATAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTAt < 1:259435/183‑1 (MQ=255) tCAACGCCGGTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGATAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACgn < 1:46358/142‑2 (MQ=255) aaCGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGATAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGt < 1:446318/251‑1 (MQ=255) gTTTATTTCTGGGGGCGAGGCCGTAGAATCACTACTTTACCCGACTGATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGTTAAGt < 1:364604/104‑1 (MQ=255) gTTTANNGCNNNGGNNGAGGCCGGATAATCGCTACTTTANCCGACGTATTGATACCGCAGCAGCGTTGTNCNANGCCGNNAAANTGAAATGGCTNCTGGTAAGt > 2:364604/1‑104 (MQ=255) | CGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAACGTGGGTTTATTGCTGGGGGCGAGGCCGGGTAATCGCTACTTTACCCGACGTATTGATACCGCAGCAGCGTTGTACCACGCCGGAAAAGTGAAATGGCTGCTGGTAAGTGGCGATAACGGACGTAAAAATTACGATGAAGCATCAGGTATGCAGCAGGCTTTGATCGCTAAAGGCGTACCAGCAAAAGTGATCTTCTGCGACTATGCCGGATTCTCAACGCTGGATTCGGT > NC_000913/3236548‑3236946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |