Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,622,358	G→A	G42D (GGC→GAC)	purN →	phosphoribosylglycinamide formyltransferase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,622,358	0	G	A	100.0%	25.3 / NA	12	G42D (GGC→GAC)	purN	phosphoribosylglycinamide formyltransferase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/10);  total (2/10)

CCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTAC  >  NC_000913/2622117‑2622588                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       cctgcctgCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAAng                                                                                                                                                                                                                               <  1:393766/251‑1 (MQ=255)     cctgcTCCGGAAGTTGACAAAGCCCGCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAATCCTCTGATTCCGAACAACGCGTGGTGATCGAATAAGGAATATTGTCGTGCTTATTTCCGGCAACGGAATTAATGTACAGGAAATTATTGTCGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGAGTTCGACCTTGAACGcgcc                                                                                                                                                                                                                           <  1:362452/251‑1 (MQ=255)                  tGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAAc                                                                                                                                                                                                                                <  1:27272/233‑1 (MQ=255)                                                                      cGGTATCATCAAATTCTCTGATGCAGAACAACGCTTGGTTATCGAATAAGTAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATGTACAGACAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCCCCTTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTGCGACCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGa                                                                                                                                                          <  1:154092/251‑1 (MQ=255)                                                                      cGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACTCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTAtn                                                                                                                                                           <  1:95912/250‑2 (MQ=255)                                                                                  aGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGcn                                                                                                                                                               <  1:311015/234‑2 (MQ=255)                                                                                                                                           tCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCCCCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTTAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCa                                                                                      <  1:442100/250‑1 (MQ=255)                                                                                                                                                          aaTTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGAACGGAGAGTTTTCCGCAATAAGGCCGAAGCGCTCGACCTTGAACGCGCCCGCCAGGCGGATCTTGCAAAGCATACGCTCAtagc                                                                                                                                                                                        >  1:464105/1‑134 (MQ=255)                                                                                                                                                                                               aaaTTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCTTTCGACCTGGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTg                                                                    <  1:230763/216‑1 (MQ=255)                                                                                                                                                                                                                              cAATAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTTGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTTCCGAAATATCCCGGATTac  <  1:411831/251‑1 (MQ=255)                                                                                                                                                                                                                                 tAAGGCCGACGCGTTCGACCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGGTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTctgn                       <  1:59424/227‑2 (MQ=255)                                                                                                                                                                                                                                         gcgcgTTCGACCTTGAACGCGCCCGCCAGGCGGGTATTGCCCCGCATACACTCAACGCCAGCGCGTTTGACAGTCGTGGAGGCTCTAAACGGGAGTTGATTAATCAAATCGAAATGTCCGCCACCGCTGTCGTCCTGCCGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCACCCCCTATGCCGGGCGTTTGCTGAACATTCACCCt                              >  1:234590/2‑212 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       CCTGCCTGCTCCGGAAGTGGACAAAGCCCTCGCCCTGCTCAATGCCAACGGTGAAAACGCGTGGAAAATCGGTATCATCAAAGCCTCTGATTCCGAACAACGCGTGGTTATCGAATAATGAATATTGTGGTGCTTATTTCCGGCAACGGAAGTAATTTACAGGCAATTATTGACGCCTGTAAAACCAACAAAATTAAAGGCACCGTACGGGCAGTTTTCAGCAATAAGGCCGACGCGTTCGGCCTTGAACGCGCCCGCCAGGCGGGTATTGCAACGCATACGCTCATCGCCAGCGCGTTTGACAGTCGTGAAGCCTATGACCGGGAGTTGATTCATGAAATCGACATGTACGCACCCGATGTGGTCGTGCTGGCTGGTTTTATGCGCATTCTCAGCCCGGCGTTTGTCTCCCACTATGCCGGGCGTTTGCTGAACATTCACCCTTCTCTGCTGCCGAAATATCCCGGATTAC  >  NC_000913/2622117‑2622588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑