breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 14,136 C→T intergenic (+57/‑32) dnaK → / → dnaJ chaperone protein DnaK/chaperone protein DnaJ
RA 23,769 C→T P460L (CCT→CTT)  ileS → isoleucine‑‑tRNA ligase
RA 38,914 C→T A68T (GCG→ACG)  caiB ← gamma‑butyrobetainyl‑CoA:carnitine CoA transferase
RA 46,186 C→T A127V (GCC→GTC)  yaaU → putative transporter YaaU
JC JC 57,276 IS2 (+) +5 bp intergenic (‑167/‑84) lptD ← / → djlA lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA
RA 60,338 C→T M3I (ATG→ATA rluA ← 23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
RA 71,134 G→A G250S (GGT→AGT)  araC → DNA‑binding transcriptional dual regulator AraC
RA 78,072 C→T S151F (TCT→TTT)  setA → sugar exporter SetA
RA 110,933 C→T C885C (TGC→TGT secA → protein translocation ATPase
RA 111,101 A→G I20V (ATA→GTA)  mutT → 8‑oxo‑dGTP diphosphatase
RA 119,204 G→A A158T (GCG→ACG)  ampD → 1,6‑anhydro‑N‑acetylmuramoyl‑L‑alanine amidase
RA 124,223 G→A G403S (GGT→AGT)  aceE → pyruvate dehydrogenase E1 component
RA 126,388 G→A V232I (GTT→ATT)  aceF → pyruvate dehydrogenase, E2 subunit
RA 129,296 G→A S462N (AGC→AAC)  lpd → lipoamide dehydrogenase
RA 131,332 G→A intergenic (‑72/‑283) yacH ← / → acnB DUF3300 domain‑containing protein YacH/AcnB
RA 135,985 G→A T160T (ACC→ACT speE ← spermidine synthase
RA 155,807 C→T S132N (AGC→AAC)  yadV ← putative fimbrial chaperone YadV
RA 158,903 C→T G75D (GGC→GAC)  pcnB ← poly(A) polymerase I
RA 197,396 C→T S284F (TCT→TTT)  rseP → intramembrane zinc metalloprotease RseP
RA 197,698 C→T L385F (CTT→TTT)  rseP → intramembrane zinc metalloprotease RseP
RA 197,886 C→T F447F (TTC→TTT rseP → intramembrane zinc metalloprotease RseP
RA 203,227 A→G Y223C (TAT→TGT)  lpxA → acyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase
RA 214,288 C→T intergenic (‑163/‑3) yaeP ← / → yaeQ PF06786 family protein YaeP/uncharacterized protein YaeQ
RA 220,003 C→T intergenic (‑8/+110) rcsF ← / ← metQ sensor lipoprotein RcsF/L‑methionine/D‑methionine ABC transporter membrane anchored binding protein
RA 229,098 C→T intergenic (+94/‑69) aspU → / → dkgB tRNA‑Asp/methylglyoxal reductase DkgB
RA 245,295 C→T V171M (GTG→ATG)  dpaA ← peptidoglycan meso‑diaminopimelic acid protein amidase A
RA 250,938 C→T A14V (GCC→GTC)  dinB → DNA polymerase IV
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 258,827 C→T pseudogene (152/331 nt) crl → RNA polymerase holoenzyme assembly factor Crl
RA 261,468 C→T R361C (CGT→TGT)  proB → glutamate 5‑kinase
RA 271,796 C→T pseudogene (33/427 nt) insN → IS911A regulator fragment
RA 293,175 C→T intergenic (‑227/+45) yagK ← / ← yagL uncharacterized protein YagK/resolvase‑like catalytic domain‑containing protein YagL
RA 296,721 C→T E126K (GAA→AAA)  intF ← putative phage integrase
RA 313,745 C→T intergenic (‑503/‑1546) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 319,185 C→T A273T (GCA→ACA)  rclA ← cupric reductase RclA
RA 334,871 C→T G51D (GGT→GAT)  yahC ← uncharacterized protein YahC
RA 353,051 C→T P116S (CCG→TCG)  prpE → propionyl‑CoA synthetase
RA 357,126 C→T P319L (CCG→CTG)  codA → cytosine/isoguanine deaminase
RA 358,860 C→T A21V (GCC→GTC)  cynT → carbonic anhydrase 1
RA 372,902 C→T A263V (GCC→GTC)  mhpD → 2‑hydroxypentadienoate hydratase
RA 400,626 C→T D100N (GAT→AAT)  ddlA ← D‑alanine‑‑D‑alanine ligase A
RA 409,743 C→T A93T (GCG→ACG)  rdgC ← nucleoid‑associated protein RdgC
RA 450,824 C→T V263M (GTG→ATG)  cyoA ← cytochrome bo3 subunit 2
RA 466,181 C→T E378K (GAA→AAA)  ybaE ← uncharacterized protein YbaE
RA 473,054 C→T A30V (GCC→GTC)  amtB → ammonium transporter
RA 473,447 C→T S161F (TCT→TTT)  amtB → ammonium transporter
RA 474,765 C→T E133K (GAA→AAA)  tesB ← acyl‑CoA thioesterase II
RA 489,602 C→T L1023L (CTG→TTG)  mscK → potassium dependent, small conductance mechanosensitive channel
RA 510,345 C→T M345I (ATG→ATA copA ← Cu(+) exporting P‑type ATPase
RA 515,644 C→T G44R (GGG→AGG)  qmcA ← PHB domain‑containing protein QmcA
RA 533,723 C→T P238L (CCT→CTT) 
noncoding (95/235 nt)
allR →
allZ →
DNA‑binding transcriptional repressor AllR
small RNA AllZ
RA 541,644 C→T L360L (CTC→CTT ybbY → putative purine transporter
RA 556,157 C→T V133I (GTC→ATC)  ybcI ← PF04307 family inner membrane protein YbcI
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
JC JC 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE
RA 580,115 C→T intergenic (+29/+136) ybcW → / ← ylcI uncharacterized protein YbcW/DUF3950 domain‑containing protein YlcI
RA 580,119 2 bp→GT intergenic (+33/+131) ybcW → / ← ylcI uncharacterized protein YbcW/DUF3950 domain‑containing protein YlcI
RA 618,222 C→T intergenic (+184/‑32) entF → / → fepE apo‑serine activating enzyme/polysaccharide co‑polymerase family protein FepE
RA 631,633 G→A W580* (TGG→TGA cstA → pyruvate transporter CstA
RA 646,088 C→T G165S (GGT→AGT)  citT ← citrate:succinate antiporter
RA 658,184 C→T intergenic (‑246/‑763) crcB ← / → tatE F(‑) channel protein/twin arginine protein translocation system ‑ TatE protein
RA 676,374 C→T E58E (GAG→GAA ybeQ ← Sel1 repeat‑containing protein YbeQ
RA 690,924 C→T Q287Q (CAG→CAA ybeX ← CorC‑HlyC family protein YbeX
RA 715,506 C→T A32V (GCT→GTT)  ybfP → lipoprotein YbfP
RA 719,561 G→A A300A (GCC→GCT speF ← inducible ornithine decarboxylase
RA 722,911 C→T A502T (GCG→ACG)  kdpD ← sensor histidine kinase KdpD
RA 723,973 C→T E148K (GAA→AAA)  kdpD ← sensor histidine kinase KdpD
RA 724,260 C→T G52E (GGG→GAG)  kdpD ← sensor histidine kinase KdpD
RA 726,513 G→A S175F (TCC→TTC)  kdpB ← K(+) transporting P‑type ATPase subunit KdpB
RA 733,473 G→A R1297R (CGG→CGA rhsC → rhs element protein RhsC
RA 735,857 G→A G70S (GGT→AGT)  ybfC → uncharacterized protein YbfC
RA 737,051 G→A intergenic (+352/‑1041) ybfQ → / → ybfD inactive transposase YbfQ/H repeat‑associated putative transposase YbfD
RA 737,311 G→A intergenic (+612/‑781) ybfQ → / → ybfD inactive transposase YbfQ/H repeat‑associated putative transposase YbfD
RA 740,913 G→A R469R (CGG→CGA phr → deoxyribodipyrimidine photolyase
RA 747,106 G→A L222F (CTC→TTC)  abrB ← putative regulator
RA 751,155 G→A Q339* (CAG→TAG)  ybgQ ← putative fimbrial usher protein YbgQ
RA 751,665 G→A P169S (CCA→TCA)  ybgQ ← putative fimbrial usher protein YbgQ
RA 752,542 G→A A85V (GCT→GTT)  ybgD ← putative fimbrial protein YbgD
RA 760,877 G→A G724G (GGG→GGA sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 761,330 G→A E875E (GAG→GAA sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 766,301 G→A P106P (CCG→CCA mngA → 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
RA 767,857 G→A G625D (GGC→GAC)  mngA → 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
RA 768,342 G→A G122D (GGT→GAT)  mngB → alpha‑mannosidase
RA 773,693 G→A A218T (GCT→ACT)  cydB → cytochrome bd‑I subunit 2
RA 774,446 G→A L46L (CTG→CTA ybgE → PF09600 family protein YbgE
RA 779,092 G→A G9E (GGG→GAG)  pal → peptidoglycan‑associated outer membrane lipoprotein Pal
RA 780,042 G→A A149T (GCT→ACT)  cpoB → cell division coordinator CpoB
RA 780,845 G→A noncoding (3/76 nt) lysW → tRNA‑Lys
RA 788,642 C→T A66T (GCC→ACC)  galM ← galactose‑1‑epimerase
RA 791,164 C→T A298T (GCC→ACC)  galE ← UDP‑glucose 4‑epimerase
RA 794,092 G→A P185S (CCG→TCG)  modE ← DNA‑binding transcriptional dual regulator ModE
RA 796,541 C→T A227V (GCG→GTG)  modB → molybdate ABC transporter membrane subunit
RA 808,338 C→T G307E (GGG→GAG)  bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 809,980 C→T P213S (CCG→TCG)  bioB → biotin synthase
RA 812,585 C→T L106F (CTT→TTT)  bioD → dethiobiotin synthetase
RA 820,340 C→T L153L (CTG→TTG)  ybhL → Bax1‑I family protein YbhL
RA 823,314 C→T E142E (GAG→GAA clsB ← cardiolipin synthase B
RA 826,170 C→T A361A (GCG→GCA ybhS ← ABC exporter membrane subunit YbhS
RA 828,038 C→T G315D (GGC→GAC)  ybhF ← ABC exporter ATP binding subunit YbhF
RA 830,226 C→T V140M (GTG→ATG)  cecR ← DNA‑binding transcriptional dual regulator CecR
RA 832,446 C→T intergenic (+210/+22) rhlE → / ← ybiA ATP‑dependent RNA helicase RhlE/N‑glycosidase YbiA
RA 832,915 C→T M12I (ATG→ATA ybiA ← N‑glycosidase YbiA
RA 832,955 C→T intergenic (‑5/‑115) ybiA ← / → dinG N‑glycosidase YbiA/ATP‑dependent DNA helicase DinG
RA 834,538 C→T S490F (TCC→TTC)  dinG → ATP‑dependent DNA helicase DinG
RA 839,888 C→T A548A (GCG→GCA fiu ← iron catecholate outer membrane transporter Fiu
RA 840,003 C→T G510D (GGC→GAC)  fiu ← iron catecholate outer membrane transporter Fiu
RA 840,587 C→T E315E (GAG→GAA fiu ← iron catecholate outer membrane transporter Fiu
RA 841,944 C→T G38D (GGT→GAT)  mcbA ← DUF1471 domain‑containing protein McbA
RA 845,100 C→T K127K (AAG→AAA ybiO ← moderate conductance mechanosensitive channel YbiO
RA 846,229 C→T A79T (GCA→ACA)  glnQ ← L‑glutamine ABC transporter ATP binding subunit
RA 855,252 C→T G165S (GGC→AGC)  ldtB ← L,D‑transpeptidase LdtB
RA 855,421 C→T M108I (ATG→ATA ldtB ← L,D‑transpeptidase LdtB
RA 857,970 C→T A364A (GCG→GCA ybiU ← DUF1479 domain‑containing protein YbiU
RA 858,144 C→T A306A (GCG→GCA ybiU ← DUF1479 domain‑containing protein YbiU
RA 858,557 C→T E169K (GAA→AAA)  ybiU ← DUF1479 domain‑containing protein YbiU
RA 860,898 C→T G570D (GGC→GAC)  ybiW ← putative pyruvate formate lyase
RA 861,220 C→T A463T (GCC→ACC)  ybiW ← putative pyruvate formate lyase
RA 862,842 C→T G224S (GGC→AGC)  ybiY ← putative pyruvate formate‑lyase activating enzyme YbiY
RA 869,891 C→T P161S (CCG→TCG)  gsiB → glutathione ABC transporter periplasmic binding protein
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 1,059,547 G→A A98T (GCG→ACG)  torA → trimethylamine N‑oxide reductase 1
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,214,463 C→T S338N (AGT→AAT)  bluF ← blue light‑ and temperature‑regulated antirepressor BluF
RA 1,247,746 C→T E457K (GAA→AAA)  dhaM ← dihydroxyacetone kinase subunit M
RA 1,290,881 C→T L214F (CTT→TTT)  rssB → regulator of RpoS
MC JC 1,294,035 Δ141 bp [tdk] [tdk]
MC JC 1,303,073 Δ12 bp coding (175‑186/921 nt) oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,353,758 T→A D254V (GAT→GTT)  sapC ← putrescine ABC exporter membrane protein SapC
RA 1,494,388 Δ1 bp coding (684/924 nt) ydcI ← DNA‑binding transcriptional dual regulator YdcI
RA 1,501,912 T→C I117I (ATT→ATC tehB → tellurite methyltransferase
RA 1,517,508 G→T V40V (GTG→GTT ortT → orphan toxin OrtT
RA 1,540,902 G→A F563F (TTC→TTT narZ ← nitrate reductase Z subunit alpha
RA 1,541,836 G→A A252V (GCC→GTC)  narZ ← nitrate reductase Z subunit alpha
RA 1,547,902 G→A G168S (GGT→AGT)  fdnG → formate dehydrogenase N subunit alpha
RA 1,573,627 G→A L526F (CTT→TTT)  pqqL ← periplasmic metalloprotease
RA 1,582,397 G→A A43V (GCT→GTT)  ydeN ← putative sulfatase YdeN
RA 1,590,212 G→A intergenic (‑211/+642) ydeT ← / ← hipA fimbrial usher domain‑containing protein YdeT/serine/threonine‑protein kinase toxin HipA
RA 1,597,469 G→A intergenic (‑5027/+1148) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,599,597 G→A P205S (CCT→TCT)  lsrK ← autoinducer‑2 kinase
RA 1,606,028 G→A E326E (GAG→GAA lsrB → Autoinducer‑2 ABC transporter periplasmic binding protein
RA 1,608,550 C→T R158H (CGT→CAT)  yneE ← PF01062 family inner membrane protein YneE
RA 1,616,014 G→A K84K (AAG→AAA yneK → protein YneK
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,887 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,716,945 G→A T93I (ACC→ATC)  tyrS ← tyrosine‑‑tRNA ligase
RA 1,744,751 Δ1 bp coding (1295/1374 nt) mdtK → multidrug efflux pump MdtK
RA 1,849,125 C→T L97L (CTG→TTG)  sppA → protease IV, a signal peptide peptidase
RA 1,850,605 C→T T590I (ACC→ATC)  sppA → protease IV, a signal peptide peptidase
RA 1,866,159 C→T R105H (CGT→CAT)  mipA ← MltA‑interacting protein
RA 1,867,262 C→T L119F (CTT→TTT)  yeaG → protein kinase YeaG
RA 1,867,508 C→T P201S (CCG→TCG)  yeaG → protein kinase YeaG
RA 1,877,158 G→A D84N (GAC→AAC)  yeaO → DUF488 domain‑containing protein YeaO
RA 1,878,257 G→A G181G (GGG→GGA dgcP → diguanylate cyclase DgcP
RA 1,879,192 G→A P22S (CCA→TCA)  yeaQ ← PF04226 family protein YeaQ
RA 1,881,996 G→A A29T (GCT→ACT)  dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
RA 1,900,052 G→A P512S (CCG→TCG)  yoaE ← TerC family inner membrane protein YoaE
RA 1,909,414 G→A P229L (CCG→CTG)  kdgR ← DNA‑binding transcriptional repressor KdgR
RA 1,912,335 G→A T81I (ACT→ATT)  htpX ← protease HtpX
RA 1,912,831 G→A I662I (ATC→ATT prc ← tail‑specific protease
RA 1,914,209 G→A A203V (GCG→GTG)  prc ← tail‑specific protease
RA 1,914,908 G→A T209T (ACC→ACT proQ ← RNA chaperone ProQ
RA 1,917,717 G→A G70S (GGC→AGC)  letB → lipophilic envelope spanning tunnel
RA 1,923,571 G→A L46L (CTG→TTG)  yebY ← DUF2511 domain‑containing protein YebY
RA 1,928,378 G→A G154G (GGC→GGT ptrB ← oligopeptidase B
RA 1,929,111 G→A P199P (CCC→CCT yebE ← DUF533 domain‑containing inner membrane protein YebE
RA 1,978,243 G→T intergenic (‑46/+275) flhD ← / ← insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
RA 2,003,264 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,242,121 A→G S283P (TCG→CCG)  yeiB ← DUF418 domain‑containing protein YeiB
JC JC 2,269,876 IS5 (+) +4 bp intergenic (+309/‑100) lpxT → / → mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
JC JC 2,319,850 IS2 (+) +5 bp coding (23‑27/2850 nt) rcsC ← histidine kinase RcsC
RA 2,328,745 G→A R351R (CGC→CGT yfaQ ← tandem DUF2300 domain‑containing protein YfaQ
RA 2,397,444 G→A Q908* (CAA→TAA)  nuoG ← NADH:quinone oxidoreductase subunit G
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,594,834 G→A T342T (ACC→ACT tmcA ← tRNA(Met) cytidine acetyltransferase
JC JC 2,666,074 IS1 (+) +9 bp coding (640‑648/1281 nt) csiE → stationary phase‑inducible protein CsiE
RA 2,705,723 G→A L468L (CTG→TTG)  lepA ← 30S ribosomal subunit biogenesis factor LepA
RA 2,712,609 G→A G52G (GGC→GGT yfiC ← tRNA m(6)A37 methyltransferase
RA 2,721,243 G→A G431R (GGA→AGA)  patZ → peptidyl‑lysine N‑acetyltransferase
RA 2,721,897 G→A D649N (GAT→AAT)  patZ → peptidyl‑lysine N‑acetyltransferase
RA 2,723,212 G→A Q162Q (CAG→CAA pssA → phosphatidylserine synthase
RA 2,724,774 G→A P325S (CCT→TCT)  kgtP ← alpha‑ketoglutarate:H(+) symporter
RA 2,724,855 G→A L298F (CTC→TTC)  kgtP ← alpha‑ketoglutarate:H(+) symporter
RA 2,729,371 G→A noncoding (74/76 nt) gltW ← tRNA‑Glu
RA 2,734,583 G→A A151V (GCC→GTC)  yfiH ← purine nucleoside phosphorylase YfiH
RA 2,738,500 G→A L252L (TTG→TTA pheA → fused chorismate mutase/prephenate dehydratase
RA 2,744,277 G→A S85F (TCT→TTT)  rplS ← 50S ribosomal subunit protein L19
RA 2,745,598 G→A Y107Y (TAC→TAT rimM ← ribosome maturation factor RimM
RA 2,755,646 G→A noncoding (54/363 nt) ssrA → tmRNA
RA 2,756,320 G→A Q54Q (CAG→CAA intA → integrase IntA
RA 2,756,503 G→A W115* (TGG→TGA intA → integrase IntA
RA 2,756,920 G→A E254E (GAG→GAA intA → integrase IntA
RA 2,760,972 G→A W142* (TGG→TGA yfjJ → protein YfjJ
RA 2,768,846 G→A G61D (GGC→GAC)  yfjQ → DUF932 domain‑containing protein YfjQ
RA 2,783,717 G→A S238F (TCC→TTC)  ypjB ← DUF5508 domain‑containing protein YpjB
RA 2,786,712 G→A intergenic (‑875/‑2273) ileY ← / → glaH tRNA‑Ile/glutarate dioxygenase GlaH
RA 2,791,708 C→T P146S (CCG→TCG)  gabD → succinate‑semialdehyde dehydrogenase (NADP(+)) GabD
RA 2,807,994 C→T A288V (GCC→GTC)  proX → glycine betaine ABC transporter periplasmic binding protein ProX
RA 2,810,638 C→T L99L (CTG→TTG)  ygaH → L‑valine exporter, YgaH component
RA 2,813,483 C→T L290L (CTG→TTG)  emrB → multidrug efflux pump membrane subunit EmrB
RA 2,819,860 C→T A718T (GCG→ACG)  alaS ← alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
RA 2,830,658 C→T A282A (GCC→GCT gutQ → D‑arabinose 5‑phosphate isomerase GutQ
RA 2,836,133 C→T W431* (TGG→TGA hypF ← carbamoyl‑‑[HypE] ligase
RA 2,837,623 T→C E161E (GAA→GAG hydN ← putative electron transport protein HydN
RA 2,838,600 C→T W222* (TGG→TAG)  ascG ← DNA‑binding transcriptional repressor AscG
RA 2,854,523 C→T Y62Y (TAC→TAT fhlA → DNA‑binding transcriptional activator FhlA
RA 2,857,938 C→T N282N (AAC→AAT mutS → DNA mismatch repair protein MutS
RA 2,865,376 C→T F92F (TTC→TTT ygbN → putative transporter YgbN
RA 2,879,047 C→T E242E (GAG→GAA cas1 ← multifunctional nuclease Cas1
RA 2,892,551 C→T T113I (ACC→ATC)  queD → 6‑carboxy‑5,6,7,8‑tetrahydropterin synthase
RA 2,897,943 C→T intergenic (‑73/+21) ygcS ← / ← ygcU putative transporter YgcS/putative FAD‑containing dehydrogenase
RA 2,906,661 C→T E427E (GAG→GAA eno ← enolase
RA 2,911,729 C→T L641L (CTG→CTA relA ← GDP/GTP pyrophosphokinase
RA 2,919,753 C→T G332R (GGG→AGG)  gudX ← glucarate dehydratase‑related protein
RA 2,921,503 C→T V200I (GTC→ATC)  gudP ← galactarate/D‑glucarate transporter GudP
RA 2,935,970 C→T S129S (AGC→AGT fucI → L‑fucose isomerase
RA 2,943,893 C→T A186V (GCT→GTT)  csdA → cysteine sulfinate desulfinase
RA 2,953,618 C→T A796T (GCG→ACG)  recB ← exodeoxyribonuclease V subunit RecB
RA 2,955,071 C→T E311E (GAG→GAA recB ← exodeoxyribonuclease V subunit RecB
RA 2,960,257 C→T Q724Q (CAG→CAA recC ← exodeoxyribonuclease V subunit RecC
RA 2,964,412 C→T E248E (GAG→GAA thyA ← thymidylate synthase
RA 2,967,640 G→A G265G (GGC→GGT ptsP ← phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 2,980,511 C→T S42N (AGC→AAC)  ygeA ← amino acid racemase YgeA
RA 2,982,637 C→T E208K (GAA→AAA)  kduD ← putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase
RA 2,983,972 C→T M51I (ATG→ATA kduI ← 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
RA 3,009,970 G→A E403K (GAG→AAG)  ygeY → putative peptidase YgeY
RA 3,018,530 G→A G824D (GGC→GAC)  ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,018,884 G→A G942D (GGC→GAC)  ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,020,602 C→T Y21Y (TAC→TAT ygfM → putative oxidoreductase
RA 3,024,475 C→T P42L (CCC→CTC)  xanQ → xanthine:H(+) symporter XanQ
RA 3,027,290 G→A G57D (GGC→GAC)  ghxQ → guanine/hypoxanthine transporter GhxQ
RA 3,035,820 G→A R154R (CGC→CGT prfB ← peptide chain release factor RF2
RA 3,040,041 G→A E63K (GAA→AAA)  fldB → flavodoxin 2
RA 3,041,375 G→A L21L (CTG→CTA ygfZ → folate‑binding protein
RA 3,043,834 G→A G58E (GGA→GAA)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,054,691 G→A F50F (TTC→TTT pepP ← Xaa‑Pro aminopeptidase
RA 3,060,430 G→A A226A (GCG→GCA argP → DNA‑binding transcriptional dual regulator ArgP
RA 3,064,539 G→A E183K (GAA→AAA)  scpB → methylmalonyl‑CoA decarboxylase
RA 3,084,483 G→A L477L (CTG→TTG)  speA ← biosynthetic arginine decarboxylase
RA 3,084,753 G→A P387S (CCG→TCG)  speA ← biosynthetic arginine decarboxylase
RA 3,087,272 G→A Q189Q (CAG→CAA metK → methionine adenosyltransferase
RA 3,090,437 G→A A31T (GCG→ACG)  endA → DNA‑specific endonuclease I
RA 3,091,050 G→A S235N (AGC→AAC)  endA → DNA‑specific endonuclease I
RA 3,093,181 G→A R82H (CGC→CAC)  yqgE → DUF179 domain‑containing protein YqgE
RA 3,099,756 G→A R325C (CGC→TGC)  ansB ← L‑asparaginase 2
RA 3,104,400 G→A intergenic (+32/‑33) yggX → / → mltC putative Fe(2(+))‑trafficking protein/membrane‑bound lytic murein transglycosylase C
RA 3,115,239 G→A P1292S (CCG→TCG)  yghJ ← putative lipoprotein YghJ
RA 3,141,250 G→A L13L (CTG→TTG)  hybD ← hydrogenase 2 maturation protease
RA 3,142,691 G→A T100I (ACC→ATC)  hybC ← hydrogenase 2 large subunit
RA 3,142,842 G→A P50S (CCG→TCG)  hybC ← hydrogenase 2 large subunit
RA 3,156,084 G→A V244I (GTC→ATC)  yqhD → NADPH‑dependent aldehyde reductase YqhD
RA 3,158,635 G→A T3T (ACG→ACA yqhH → lipoprotein YqhH
RA 3,167,670 G→A P17S (CCT→TCT)  ygiS ← putative deoxycholate binding periplasmic protein
RA 3,171,411 G→A E310K (GAG→AAG)  qseC → sensor histidine kinase QseC
RA 3,225,963 C→T A81V (GCG→GTG)  ebgC → DUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta
RA 3,227,114 C→T T294I (ACC→ATC)  ygjI → putative transporter YgjI
RA 3,250,599 G→A R13H (CGC→CAC)  yqjF → DoxX family protein
RA 3,253,119 G→A R70H (CGC→CAC)  yhaI → putative inner membrane protein
RA 3,268,970 A→G T186A (ACA→GCA)  yhaC → uncharacterized protein YhaC
RA 3,439,389 G→A P41S (CCG→TCG)  yhdN ← DUF1992 domain‑containing protein YhdN
RA 3,441,396 G→A L94L (CTG→TTG)  rpsD ← 30S ribosomal subunit protein S4
RA 3,450,847 G→A T173I (ACC→ATC)  rplB ← 50S ribosomal subunit protein L2
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,510,022 C→T E319K (GAA→AAA)  yhfX ← putative PLP‑binding protein YhfX
RA 3,512,791 C→T G283D (GGT→GAT)  trpS ← tryptophan‑‑tRNA ligase
RA 3,513,204 C→T P145P (CCG→CCA trpS ← tryptophan‑‑tRNA ligase
RA 3,514,681 C→T D127N (GAT→AAT)  rpe ← ribulose‑phosphate 3‑epimerase
RA 3,519,285 C→T intergenic (‑221/+180) aroK ← / ← hofQ shikimate kinase 1/DNA utilization protein HofQ
RA 3,523,837 C→T L323L (CTG→TTG)  mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,528,294 C→T A609V (GCT→GTT)  igaA → inner membrane protein IgaA
RA 3,537,058 G→A G83S (GGC→AGC)  greB → transcription elongation factor GreB
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,569,134 C→T G65G (GGG→GGA glgC ← glucose‑1‑phosphate adenylyltransferase
RA 3,569,284 C→T Q15Q (CAG→CAA glgC ← glucose‑1‑phosphate adenylyltransferase
RA 3,592,762 C→T A226T (GCG→ACG)  livF ← branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivF
RA 3,632,560 C→T D11N (GAC→AAC)  yhiJ ← DUF4049 domain‑containing protein YhiJ
MC JC 3,682,261 Δ34 bp coding (1154‑1187/1287 nt) dctA ← C4 dicarboxylate/orotate:H(+) symporter
JC JC 3,737,137 IS2 (+) +5 bp coding (37‑41/825 nt) bax ← putative glycoside hydrolase Bax
RA 3,931,737 C→T T141I (ACC→ATC)  kup → K(+):H(+) symporter Kup
JC JC 4,123,923 IS2 (–) +5 bp coding (530‑534/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
JC JC 4,135,180 IS2 (–) +5 bp coding (1346‑1350/2181 nt) katG → catalase/hydroperoxidase HPI
RA 4,146,389 (T)6→7 coding (132/879 nt) pflC → putative pyruvate formate‑lyase 2 activating enzyme PflC
RA 4,215,023 C→T F248F (TTC→TTT metA → homoserine O‑succinyltransferase
RA 4,215,796 C→T L107F (CTC→TTC)  aceB → malate synthase A
RA 4,217,843 C→T C245C (TGC→TGT aceA → isocitrate lyase
RA 4,218,459 C→T intergenic (+46/‑137) aceA → / → aceK isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase
RA 4,228,406 C→T L226F (CTC→TTC)  yjbB → putative inorganic phosphate export protein YjbB
RA 4,237,200 C→T Q102* (CAG→TAG)  yjbG → capsule biosynthesis GfcC family protein YjbG
RA 4,243,528 C→T intergenic (‑12/+3) malG ← / ← malF maltose ABC transporter membrane subunit MalG/maltose ABC transporter membrane subunit MalF
RA 4,253,284 C→T A90V (GCG→GTG)  ubiA → 4‑hydroxybenzoate octaprenyltransferase
RA 4,253,343 C→T L110L (CTG→TTG)  ubiA → 4‑hydroxybenzoate octaprenyltransferase
RA 4,346,121 C→T E402E (GAG→GAA fumB ← fumarase B
RA 4,347,028 C→T G100D (GGC→GAC)  fumB ← fumarase B
RA 4,366,530 G→A R82C (CGT→TGT)  dcuA ← C4‑dicarboxylate transporter DcuA
RA 4,369,674 C→T E247K (GAA→AAA)  yjeH ← L‑methionine/branched chain amino acid exporter
RA 4,376,438 G→A S41N (AGC→AAC)  ecnA → entericidin A lipoprotein, antidote to entericidin B
RA 4,464,811 G→A T457I (ACC→ATC)  treB ← trehalose‑specific PTS enzyme IIBC component
RA 4,472,103 G→A T98I (ACT→ATT)  pyrB ← aspartate carbamoyltransferase catalytic subunit
RA 4,473,265 G→A intergenic (‑56/+75) yjgH ← / ← bdcA RutC family protein YjgH/c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,482,228 G→A P537L (CCG→CTG)  valS ← valine‑‑tRNA ligase
RA 4,487,189 G→A K324K (AAG→AAA lptF → lipopolysaccharide transport system protein LptF
RA 4,496,246 G→A intergenic (‑37/‑159) ahr ← / → leuX NADPH‑dependent aldehyde reductase Ahr/tRNA‑Leu
RA 4,497,794 G→A intergenic (+1305/‑478) leuX → / → insC6 tRNA‑Leu/IS2 insertion element repressor InsA
RA 4,497,882 G→A intergenic (+1393/‑390) leuX → / → insC6 tRNA‑Leu/IS2 insertion element repressor InsA
RA 4,499,871 G→A intergenic (+371/‑1389) insD6 → / → yjgZ IS2 insertion element protein InsB/uncharacterized protein YjgZ
RA 4,506,221 G→A R312K (AGA→AAA)  nanY → 2,7‑anhydro‑N‑acetylneuraminate hydratase
RA 4,518,915 G→A intergenic (+113/+423) insA7 → / ← yjhU IS1 family repressor protein InsA/putative DNA‑binding transcriptional regulator YjhU
RA 4,519,461 G→A F288F (TTC→TTT yjhU ← putative DNA‑binding transcriptional regulator YjhU
RA 4,527,451 G→A S29S (AGC→AGT sgcE ← putative epimerase SgcE
RA 4,529,464 G→A I260I (ATC→ATT sgcC ← putative PTS enzyme IIC component SgcC
RA 4,535,060 G→A intergenic (‑1007/‑1537) topAI ← / → ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
RA 4,535,672 G→A intergenic (‑1619/‑925) topAI ← / → ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
RA 4,536,025 G→A intergenic (‑1972/‑572) topAI ← / → ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
JC JC 4,536,236 IS5 (+) +4 bp intergenic (‑2183/‑358) topAI ← / → ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
RA 4,542,683 Δ1 bp intergenic (+50/‑432) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit
RA 4,544,956 G→A G218E (GGA→GAA)  fimC → type 1 fimbriae periplasmic chaperone
RA 4,547,328 G→A A745T (GCC→ACC)  fimD → type I fimbriae usher protein
RA 4,547,862 G→A A41T (GCC→ACC)  fimF → type 1 fimbriae minor subunit FimF
MC JC 4,549,192 Δ39 bp coding (385‑423/903 nt) fimH → type 1 fimbriae D‑mannose specific adhesin
RA 4,562,620 C→T G19E (GGG→GAG)  yjiJ ← putative transporter YjiJ
RA 4,569,129 C→T intergenic (‑402/+1033) ytiE ← / ← yjiR protein YtiE/fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
RA 4,577,700 G→A L87F (CTT→TTT)  mcrC ← Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit
RA 4,577,948 G→A P4L (CCC→CTC)  mcrC ← Type IV methyl‑directed restriction enzyme EcoKMcrBC subunit
RA 4,598,800 G→A G214G (GGC→GGT opgB ← phosphoglycerol transferase I
RA 4,600,930 G→A P16S (CCT→TCT)  dnaC ← DNA replication protein DnaC
RA 4,602,252 G→A L203L (CTG→TTG)  yjjP ← putative succinate exporter YjjP
RA 4,606,093 G→A noncoding (73/87 nt) leuV ← tRNA‑Leu
RA 4,610,833 G→A V474I (GTA→ATA)  prfC → peptide chain release factor RF3
RA 4,615,442 G→A R34R (CGC→CGT yjjW ← putative glycyl‑radical enzyme activating enzyme YjjW
RA 4,617,261 G→A intergenic (‑196/‑62) yjjI ← / → deoC DUF3029 domain‑containing protein YjjI/deoxyribose‑phosphate aldolase
RA 4,617,368 G→A D16N (GAC→AAC)  deoC → deoxyribose‑phosphate aldolase
RA 4,636,144 G→A Q46Q (CAG→CAA creB → DNA‑binding transcriptional regulator CreB
RA 4,639,804 G→A A168V (GCC→GTC)  arcA ← DNA‑binding transcriptional dual regulator ArcA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 118574 118634 61 2 [1] [1] 2 nadC quinolinate phosphoribosyltransferase (decarboxylating)
* * ÷ NC_000913 164549 164638 90 2 [1] [1] 2 hrpB/mrcB RNA‑dependent NTPase HrpB/peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
* * ÷ NC_000913 245695 245742 48 2 [1] [1] 2 dpaA peptidoglycan meso‑diaminopimelic acid protein amidase A
* * ÷ NC_000913 332863 332978 116 2 [1] [1] 2 pdeL DNA‑binding transcriptional regulator/c‑di‑GMP phosphodiesterase PdeL
* * ÷ NC_000913 384663 384719 57 2 [1] [0] 2 yaiS/tauA putative deacetylase YaiS/taurine ABC transporter periplasmic binding protein
* * ÷ NC_000913 652197 652213 17 3 [1] [1] 2 citC/dpiB citrate lyase synthetase/sensor histidine kinase DpiB
* * ÷ NC_000913 807338 807360 23 2 [1] [1] 2 ybhC/ybhB outer membrane lipoprotein YbhC/putative kinase inhibitor
* * ÷ NC_000913 1107522 1107620 99 3 [1] [1] 2 clsC cardiolipin synthase C
* * ÷ NC_000913 1194254 1194308 55 2 [1] [1] 2 [nudJ]–[rluE] [nudJ],[rluE]
* * ÷ NC_000913 1196371 1211432 15062 2 [1] [1] 2 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1254788 1254835 48 2 [1] [1] 2 ycgV putative autotransporter adhesin YcgV
* * ÷ NC_000913 1299488 1300693–1299494 7–1206 2 [1] [0] 2 insH21 insH21
* * ÷ NC_000913 1331647 1331707 61 2 [0] [1] 2 topA DNA topoisomerase 1
* * ÷ NC_000913 1351182 1351222 41 2 [1] [1] 2 fabI/ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
* * ÷ NC_000913 1353263 1353304 42 2 [1] [0] 2 sapD putrescine ABC exporter ATP binding protein SapD
* * ÷ NC_000913 1491579 1491638 60 2 [1] [1] 2 rydC/ydcA small regulatory RNA RydC/protein YdcA
* * ÷ NC_000913 1536797 1536830 34 2 [1] [1] 2 narW NarW, putative private chaperone for NarZ nitrate reductase subunit
* * ÷ NC_000913 1606169 1606204 36 2 [1] [1] 2 lsrF 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase
* * ÷ NC_000913 1787358 1787385 28 2 [1] [1] 2 ppsA/ppsR phosphoenolpyruvate synthetase/phosphoenolpyruvate synthetase regulatory protein
* * ÷ NC_000913 1833772 1833846 75 2 [1] [1] 2 ydjX DedA family protein YdjX
* * ÷ NC_000913 1978495 1979270 776 6 [0] [0] 14 insB5–insA5 insB5,insA5
* * ÷ NC_000913 1994494 1994679 186 3 [1] [1] 2 uvrC UvrABC excision nuclease subunit C
* * ÷ NC_000913 2052482 2052546 65 2 [1] [1] 2 yeeJ/shiA inverse autotransporter adhesin/shikimate:H(+) symporter
* * ÷ NC_000913 2083735 2083745 11 3 [1] [0] 2 sbcB exodeoxyribonuclease I
* * ÷ NC_000913 2196896 2197019 124 2 [1] [1] 2 yehF PF05406 family protein YehF
* * ÷ NC_000913 2264705 2264716 12 2 [1] [1] 2 setB sugar exporter SetB
* * ÷ NC_000913 2342395 2342491 97 3 [1] [1] 2 yfaL putative autotransporter adhesin YfaL
* * ÷ NC_000913 2411769 2411836 68 3 [1] [1] 2 hxpA hexitol phosphatase A
* * ÷ NC_000913 2623553 2623586 34 2 [1] [1] 2 ppk polyphosphate kinase
* * ÷ NC_000913 2939694 2939720 27 2 [1] [0] 2 fucR DNA‑binding transcriptional activator FucR
* * ÷ NC_000913 3131480 3131532 53 2 [1] [1] 2 yghQ putative transport protein YghQ
* * ÷ NC_000913 3239585 3239693 109 2 [1] [1] 2 alx/sstT putative membrane‑bound redox modulator Alx/serine/threonine:Na(+) symporter
* * ÷ NC_000913 3423841–3424229 3424522–3424240 12–682 3 [1] [1] 2 [rrlD] [rrlD]
* * ÷ NC_000913 3477913 3477969 57 2 [1] [1] 2 slyD FKBP‑type peptidyl‑prolyl cis‑trans isomerase SlyD
* * ÷ NC_000913 3543180 3543312 133 2 [1] [1] 3 rpnA recombination‑promoting nuclease RpnA
* * ÷ NC_000913 3560808 3560884 77 2 [1] [1] 2 glpG rhomboid protease GlpG
* * ÷ NC_000913 3769348 3769349 2 2 [0] [1] 2 yibV PF15596 family protein YibV
* * ÷ NC_000913 4165711 4165739 29 2 [1] [1] 2 murI glutamate racemase
* * ÷ NC_000913 4331699 4331804 106 2 [1] [0] 2 proP osmolyte:H(+) symporter ProP

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 382179 =NA (NA)14 (1.400) 11/294 0.1 NA noncoding (920/1331 nt) IS2 repeat region
?NC_000913 = 382590 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 1397238NA (NA)6 (0.590) 6/298 0.3 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 = 1651548NA (NA)12 (1.180) 9/298 0.1 92.3% noncoding (706/706 nt) IS2 repeat region
?NC_000913 = 1792776 1 (0.100)intergenic (‑45/+33) nlpC/btuD NlpC/P60 family lipoprotein NlpC/vitamin B12 ABC transporter ATP binding subunit
* ? NC_000913 1979271 =0 (0.000)15 (1.480) 13/298 0.0 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL