Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	353,051	C→T	P116S (CCG→TCG)	prpE →	propionyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	353,051	0	C	T	100.0%	15.5 / NA	8	P116S (CCG→TCG)	prpE	propionyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/3);  total (5/3)

GTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCGTGGCGGCGCGAATTGATGACGCTAAACCGGTGCTGATTGTCTCGGCTGATGCCGGAGCGCGCGGTGGCAAAATCATTCCCTATAAAAAATTG  >  NC_000913/352840‑353236                                                                                                                                                                                                                    |                                                                                                                                                                                          gTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGTCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTgcg                                                                                                                                                    >  1:91355/1‑251 (MQ=255)                         gtgCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAAAAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGTCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCgg                                                                                                                            >  1:184016/1‑251 (MQ=255)                                                    ggAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGTCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCGcac                                                                                                  >  1:17138/1‑250 (MQ=255)                                                                                          ctTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGTCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCGTGGCGGCGCGAATTGATGACGCTAAACCGGTg                                                              <  1:434/248‑1 (MQ=255)                                                                                                                              gTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGTCGATGATTGCCGAAGCGCATATTActctn                                                                                                                                                              <  1:75986/116‑2 (MQ=255)                                                                                                                                                                                      cAGCGCGGCGATCGGGTGCTGGTGTATATGTCGATGATTGCCGAAGCGCATATTACACTGATGCCATGCGCGAGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCATCGCACAGCGTGGGGGCGCGAATTGATGACg                                                                         >  1:244817/1‑145 (MQ=255)                                                                                                                                                                                         ngcggcgATCGGGTGCTGGTGTATATGTCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCGTGGCGGCGCGAATTGATGACGCTAAACCGGTGCTGATTGTCTCGGCTGATGCCGGAGCGCGCGGTGGCAAAATCATTCCCTATAAAAAATTg  >  1:158698/2‑213 (MQ=255)                                                                                                                                                                                         cgcgGCGATCGGGTGCTGGTGTATATGTCGATTATTGCCGAAGCGCATATTACTCTGCTTGCCTGCGCGCGCATTTGCGCTATTCACGCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCGTggc                                                                                          <  1:232198/125‑1 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                          GTTTTGTGAAGGCCGAACCAACTTGTGCCACAACGCCATCGACCGCTGGCTGGAGAAACAGCCAGAGGCGCTGGCGCTGATTGCCGTCTCTTCGGAAACAGAAGAAGAGCGCACCTTTACCTTTCGTCAGCTGCATGACGAAGTGAACGCGGTGGCCTCAATGTTGCGTTCATTGGGTGTGCAGCGCGGCGATCGGGTGCTGGTGTATATGCCGATGATTGCCGAAGCGCATATTACTCTGCTGGCCTGCGCGCGCATTGGCGCTATTCACTCGGTGGTGTTTGGTGGATTTGCCTCGCACAGCGTGGCGGCGCGAATTGATGACGCTAAACCGGTGCTGATTGTCTCGGCTGATGCCGGAGCGCGCGGTGGCAAAATCATTCCCTATAAAAAATTG  >  NC_000913/352840‑353236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑