Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,935,970	C→T	S129S (AGC→AGT)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,935,970	0	C	T	100.0%	26.7 / NA	12	S129S (AGC→AGT)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

GCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTG  >  NC_000913/2935725‑2936207                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              gCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTAAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCAATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGTCAGa                                                                                                                                                                                                                                           >  1:177358/1‑250 (MQ=255)     gCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGGAGCGCGGGTATGTCTGATGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCCTCATTTCGGTAACGCCTTGCTGGTGCTAGGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGTCCATTTGTGGCTGTAACGTCTCGGAACGGCCCGGCGCTGTTTATCTGGCAGCGGCTCTGGCAGCTCACAGTCAGAAAGGc                                                                                                                                                                                                                                      <  1:147037/251‑1 (MQ=255)                   gAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGTCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGTCAGAAAGGCATccc                                                                                                                                                                                                                                 <  1:85281/242‑1 (MQ=255)                                                                        cGAAGAAAAATTCAGCAGTCAGCATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAACCCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCGCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGTCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATg                                                                                                                                                                                        >  1:228221/1‑230 (MQ=255)                                                                                             gAANGNNGNNCGCNCCATTACGGTAACGCCTTGCTNNTGCTATGGCNGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACNGCACTGAACNNCCNGGCGCNGNNNANNNGGNAGCGGCTCTGGCAGCTCACNGTNNGAAAGGNATCCCAGCATTCTCCATTTACGGTCa                                                                                                                                                                                                            <  2:58514/189‑1 (MQ=255)                                                                                                                         ncTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGTCAGAAAGGCATCCCAGCATTCTCCAtt                                                                                                                                                                                                                    >  1:157835/2‑153 (MQ=255)                                                                                                                                nnggNNNNNGCANGGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCAGTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGTCAGAAAGGCATCCCAGCATTCTCCAtt                                                                                                                                                                                                                    <  2:157835/143‑1 (MQ=255)                                                                                                                                              naaaCCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGTCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCg                                                                                                                         >  1:154566/2‑223 (MQ=255)                                                                                                                                                                                     tGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGTCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGggcggcn                                                                               <  1:30345/226‑2 (MQ=255)                                                                                                                                                                                                            ccccGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGTCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAGGTAAAACTGNTGCNNNNNGc                                                                                                                                          >  2:153704/1‑144 (MQ=255)                                                                                                                                                                                                                       nnnnnntGNNNNNGGCNCTGGCAGCTCACAGTCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCg                                                                                                                         <  2:154566/144‑1 (MQ=255)                                                                                                                                                                                                                                          ggCAGCTCACAGTCAGAAAGGCATCCCAGCATCCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTg  <  1:231871/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              GCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACCTGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTAGGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGATCCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTACCTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGTCATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTG  >  NC_000913/2935725‑2936207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑