Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,983,972	C→T	M51I (ATG→ATA)	kduI ←	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,983,972	0	C	T	100.0%	19.8 / NA	9	M51I (ATG→ATA)	kduI	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/5);  total (4/5)

GCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAAC‑TTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACC  >  NC_000913/2983733‑2984131                                                                                                                                                                                                                                                 |                                                                                                                                                                gcgaatttttaaaaaaaCTTCGCCGGAGGGCGGGTATAAATACTGGCAAAAAAACC‑TTCTTTTGCTCCTTTCACAACAAACAGGTCGTCGCGGTAACAATTTTCATAGAATTGGACTTCGACAATAATCATCCCGGCACCGCCAATATGGATAACACCTAACTCCCGACGTTTAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCTCCAACGGAAACCGTTTTAGTTATCGGTATAATgccgcc                                                                                                                                                      <  1:231803/238‑1 (MQ=255)  cgcAATTGTAATAAAACTTCGCCGGAGTGCCGGTCTCAATACTGGCAAAGA‑AACATTCTTTTGCACCTTTACCACCATACAGGGCGTCGCGGTGACCGCTTTCCTAGCATTAGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGTGTAATgccgcc                                                                                                                                                      >  1:103336/1‑250 (MQ=255)                      gCCGGAGGGCCGGTAAAAATACTGGAAAAAACAAC‑TTTTTTTGCCCCGTTACCAACATCCGGGGCGTCGCGGTGACCGATTTCATAGCTTTGGCCATCGACAGTATTCGCACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTTTTTACCAACTTCCCCGCCAACGGAATCCGTTTTAGTTATCGGTATAATGCCGCCAACAATAATTCGGTCAAtgtg                                                                                                                                 <  1:111295/251‑1 (MQ=255)                                                 aaGACACC‑TTCTTTTGCACTTTTACCAACATCCAGGGCGTCGCGGTGCCCGATTTCATAGCATTGGCAATCGACAGTAATCGTCCCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGTATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGc                                                                                                      <  1:85852/251‑1 (MQ=255)                                                                                            nggTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGACATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGTATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCa                                                                                      >  1:25644/2‑225 (MQ=255)                                                                                                          aTAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCAAATATTGATACCACCTAACTCGAGAAGTTCAAGGAAATGGCTTCCGCAGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGTATCATGACGCCACCAATAATTCGGCCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAAACAAAAATTCATTGCGCAGCCCTTGGGAATCCAGCGttt                                              >  1:127711/1‑251 (MQ=255)                                                                                                                                      ccGGCACCGCCAATATTGATAACACCTAACTCGCTACGTTCAAGGAAATAGCTTGCGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGTATAATGCCGCCAACAATAATTCGGTCAATGGGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTGTCAACCAAAAATTCAGTGCTCAGCCCTTGGGTGTCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTc                   <  1:221823/250‑1 (MQ=255)                                                                                                                                                       gATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTTTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGTATAATGCCGCCAACAATAATTCGGTCAATGTGGCGGTAAACCATGGTGTACTCATCGGCGACAAATACCTTGTCAACCAAAAAGTCATTGCGCAGCCCTTGGTTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAAcc  <  1:37068/250‑1 (MQ=255)                                                                                                                                                                                             cGCCGAGTTGGTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTACGGGTATAATGACGCCAACCATAATTCGGTCAATGTGGCTGTAAACCATGGCGTACTCATCGGCGACAAAt                                                                                               >  1:163886/1‑119 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                GCGCAATTGTAATAAAACTTCGCCGGAGTGCCGGTATCAATACTGGCAAAGACAAC‑TTCTTTTGCACCTTTACCAACATACAGGGCGTCGCGGTGACCGATTTCATAGCATTGGCCATCGACAGTAATCGTACCGGCACCGCCAATATTGATAACACCTAACTCGCGACGTTCAAGGAAATAGCTTACGCCGAGTTGTTTACCAACTTCCCCGCCAACGGAAACCGTTTTAGTTATCGGCATAATGCCGCCAACAATAATTCGGTCAATGTGGCTGTAAACCATGGTGTACTCATCGGCGACAAATACCTTTTCAACCAAAAATTCATTGCGCAGCCCTTGGGTATCCAGCGTTTTTGCGTGCGCACTGTGGATGCTCTGTCTTACGTCCACATCAACC  >  NC_000913/2983733‑2984131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑