Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,115,239	G→A	P1292S (CCG→TCG)	yghJ ←	putative lipoprotein YghJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,115,239	0	G	A	100.0%	20.6 / NA	10	P1292S (CCG→TCG)	yghJ	putative lipoprotein YghJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

TGCCTGGTTGTTGCTCTCCTCCAGATATTCCGGTGCGACGGTAATATCGTCAGCGACACGGTTCATCTTGCCGAGATAACGATCCTGCATGTACAGCGCCAGCACGTTGTTAGCGACTTCAGTTGCACCCGGTACAGTCAACGGCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGC  >  NC_000913/3115033‑3115472                                                                                                                                                                                                               |                                                                                                                                                                                                                                          nnnnngnTTNNTGCTNTCCTCCAGATATTCCGGTGCGACGNTAATATCGTCAGCGACACGGTTCATCTTGACNANATAACGATCCTGCATGTACAGCGCCAGCACGTTGTTAGCGACTTCAGTNGCACCCGGTANAGTNNACGGNGNTNCTGCGGCGTTATGACCGACTTCATGCCAGNTCAGCCAGTCGTTCAGCGGCGTCNNCGNNNGCNNGGTGc                                                                                                                                                                                                                                <  2:187678/213‑1 (MQ=255)                                                            ggTTCATCTTGCCGAGATAACGATCCTGCATGTACAGCGCCAGCACGTTGTTAGCGACTTCAGTTGCACCCGGTACAGTCAACGGCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGACAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTgg                                                                                                                                    >  1:3973/1‑251 (MQ=255)                                                                   cTTGCCGAGATAACGATCCTGCATGTACAGCGCCAGCACGTTGTTAGCGACTTCAGTTGCACCCGGTCCAGTCAACGCCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGACAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATAACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGc                                                                                                                              >  1:138042/1‑250 (MQ=255)                                                                                                   ccAGCACGTTGTTAGCGACTTCAGTTGCACCCGGTACAGTCAACGGCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGACAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCg                                                                                             <  1:2207/251‑1 (MQ=255)                                                                                                                     cTTCAGTTGCACCAGGTACAGTCAACGGCGTTTCTGCGGCGTTATGACCGACTTCATTCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGACAGCGTGGTGCTGTTCGGCGAGAAGCGGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGtt                                                                                                                                         <  1:211853/189‑1 (MQ=255)                                                                                                                               ncccGGTACAGTCAACGGCGTTTCTGCGGCGTTATGACCGACTTCATGACAGATCAGCCAGTCGTTCAGCGGCGTCGTCGACAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAAc                                                                                                                                               >  1:28286/2‑173 (MQ=255)                                                                                                                                                       tGCGGCGTTATGAACGACTTCATGCCAGATCAGCCCGTAGTTCAGCGGCGTCGTCAAAAACGTGGTGCTGATCGGCGCGAAGCTGCTGTTCCTTACCGGATAACACGGATGCGCATCAACGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGAAAGTTTTTATAGGGAAACATCAGGTGCTTACCGTCTTCGCTATCAAGGCCGGAGAAGTCATTCATAGCGCTGGAACAGGTATcc                                          >  1:185012/1‑250 (MQ=255)                                                                                                                                                                   aCCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGACAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCgg                                                                                                                               >  1:15170/1‑153 (MQ=255)                                                                                                                                                                                      aGCCAGTCGTTCAGCGGCGTCGTCGACAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCg                                                                                                                                <  1:17456/133‑1 (MQ=255)                                                                                                                                                                                              gTTCAGCGGCGTCGTCGACAGCGTGGTTCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGGAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTgn  <  1:157523/251‑2 (MQ=255)                                                                                                                                                                                                       ngtcgtcGACAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATc                                                                                                                                                                  >  1:66281/2‑82 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                          TGCCTGGTTGTTGCTCTCCTCCAGATATTCCGGTGCGACGGTAATATCGTCAGCGACACGGTTCATCTTGCCGAGATAACGATCCTGCATGTACAGCGCCAGCACGTTGTTAGCGACTTCAGTTGCACCCGGTACAGTCAACGGCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATTGC  >  NC_000913/3115033‑3115472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑