Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	473,054	C→T	A30V (GCC→GTC)	amtB →	ammonium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	473,054	0	C	T	100.0%	11.8 / NA	6	A30V (GCC→GTC)	amtB	ammonium transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/3);  total (3/3)

TGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGTTTTACGGTGGGTTGATTCGCGGCAA  >  NC_000913/472941‑473147                                                                                                                  |                                                                                              nggCACAAAGCAACAGGAACGAAAAATGAAGATCGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTACTGGCTGCACCTGCGGTGGCCGATAAAGTCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGAc                                               >  1:33371/2‑162 (MQ=255)   gCACACAGCAACAGGAACGAAAAATGAAGATACCGCCGATAAAAACTGGGCTTGCTTCACTGTCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGTCGACAATGCGTTAATGATGATTTGAACTGCCCTGGTGCTGTTTATGACTATTCCGGTGATTGCC‑TGTTTTACGGTGGGTTGATTcgcg      <  1:172082/200‑1 (MQ=255)                                                        ncttCACTGGCGCTGATTCCGGGCCTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGTCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGttt                         >  1:68538/2‑129 (MQ=255)                                                         nttCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGTCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGTTTTACGGTGGGGTGATTCGCGGCaa  >  1:156155/2‑151 (MQ=255)                                                              nnggNNNNNCTTNCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGTCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGTTTTACGGTGGGTTGATTCGCGGCaa  <  2:156155/144‑1 (MQ=255)                                                                                 tGGTAATGGCTGCACCTGCGGTGGCCGATAAAGTCGACAATGCGTTTATGATGATTTGTACGGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGTTTTACGGTGGGTTGATTcgcg      <  1:139473/123‑1 (MQ=255)                                                                                                                  |                                                                                              TGGCACACAGCAACAGGAACGAAAAATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTAATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCGCTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGTTTTACGGTGGGTTGATTCGCGGCAA  >  NC_000913/472941‑473147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑