Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	722,911	C→T	A502T (GCG→ACG)	kdpD ←	sensor histidine kinase KdpD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	722,911	0	C	T	100.0%	31.0 / NA	12	A502T (GCG→ACG)	kdpD	sensor histidine kinase KdpD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

GTCATTCCTTGCGGATGTGTTAACGGCTGCAATTTACCGTTGTCATCGGGCAACAACACCTGACTGCGGGCATGAAACGTGGAGGCAATAAATTGTTCGCTGGTGGCAGCGATATCCTGCGGACTGCGGCCCACCGCCAGAGCTTTCGACATTTCATATAAGTGCCGTGTGCGTTGCTCGCGGTAACGGGCTACCCGCGCCTGATAACGCACGCCAGCAGTAAGGTTCCCGATCACCAGCCCGACGGTTAACATCACCGCGAAGGTCAGCAGATATTGCACATCAGAGACGGCGAGCGTGCCGCGTGGGGCGATAAAAAAGAGATCGAAACTCACTACATTAATGACGGTGGCAACCACTGAAGGCCAGCGTCCATAAAATAGCGCCACCACCACCACGCCAAGCAGATACAGCATCACCAGGTTGGCGGCATCAAACG  >  NC_000913/722712‑723150                                                                                                                                                                                                        |                                                                                                                                                                                                                                                ntCATTCCTTGCGGATGTGTTAACGGCTGCAATTTACCGTTGTCATCGGGCAACAACACCTGACTGCGGGCATGAAACGTGGAGGCCATAAATTGTTCGCTGGTGGCAGCGATATCCTGCGCACTGCGGCCCACCGCCAGAGCTTTCGACATTTCATATAAGTGCCGTGTGCGTTGCTCGCGGCAACGGGCTACCCGCGTCTGATAACGCACGCCAGCAGTAAGGTTCCCGATCAcc                                                                                                                                                                                                            >  1:50449/2‑237 (MQ=255)                               aaTTTACCGTTGTCATCGGGCAACAACACCTGACTGCGGGCATGAAACGTGGAGGCAATAAATTGTTCGCTGGTGGCAGCGATATCCTGCGGACTGCGGCCCACCGCCAGAGCTTTCGACATTTCATATAAGTGCCGTGTGCGTTGCTCGCGGTAACGGGCTACCCGCGTCTGATAACGCACGCCAGCAGTAAGGTTCCCGATCACCAGCCCGACGGTTAACATCACCGCGAAGGTCAGCAGATATTGcan                                                                                                                                                                <  1:68057/251‑2 (MQ=255)                                                            ntGACTGCGGGCATGAAACGTGGAGGCAATAAATTGTTCGCTGGTGGCAGCGATATCCTGCGGACTGCGGCCCACCGCCAGAGCTTTCGACATTTCATATAAGTGCCGTGTGCGTTGCTCGCGGTAACGGGCTACCCGCGTCTGATAACGCACGCCAGCAGTAAGGTTCCCGATCACCAGCCCGACGGTTAACATCACCGCGAAGGTCAGCAGat                                                                                                                                                                       >  1:63933/2‑215 (MQ=255)                                                                     ggCATGAAACGTGGAGGCAATAAATTGTTCGCTGGTGGCAGCGATATCCTGCGGACTGCGGCCCACCGCCAGAGCTTTCGACATTTCATATAAGTGCCGTGTGCGTTGCTCGCGGTAACGGGCTACCCGCGTCTGATAACGCACGCCAGCAGTAAgg                                                                                                                                                                                                                        >  1:78909/1‑157 (MQ=255)                                                                                                             gCGATNNCCTGCGGACNGCGGCCCACCGCCAGAGCTTTCGACATTTCATATAAGTGCCGTGTGCGTTGCTNGCGGTAACGNNCTNCCCGCNTNNNANNNCGNACGCCAGCAGTAAGGTTCCNGANNACCAGCNCGACGGTTAACATCACCGCGAAGGTc                                                                                                                                                                              <  2:61157/159‑1 (MQ=255)                                                                                                             gCGATATCCTGCGGACTGCGGCCCACCGCCAGAGCTTTCGACATTTCATATAAGTGCCGTGTGCGTTGCTCGCGGTAACGGGCTACCCGCGTCTGATAACGCACGCCAGCAGTAAGGTTCCCGATCACCAGCCCGACGGTTAACATCACCGCGAAGGTc                                                                                                                                                                              >  1:61157/1‑159 (MQ=255)                                                                                                                          gACTGCGGCCCACCGCCAGAGCTTTCGACATTTCGTATAAGTGCCGTGTGCGTTGCTCGCGGTAACGGGCTACCCGCGTCTGATAACGCACGCCAGCAGTACGGt                                                                                                                                                                                                                       >  2:158431/1‑105 (MQ=255)                                                                                                                          gACTGCGGCCCACCGCCAGAGCTTTCGACATTTCATATAAGTGCCGTGTGCGTTGCTCGCGGTAACGGGCTACCCGCGTCTGATAACGCACGCCAGCAGTAAggn                                                                                                                                                                                                                       <  1:158431/105‑2 (MQ=255)                                                                                                                              gCGGCCCACCGCCAGAGCTTTCGACATTTCATATAAGTGCCGTGTGCGTTGCTCGCGGTAACGGGATACCCGCGTCTGATAACGCACGCCAGCCGTAAGGTTCCCGATCACCAGCCCGACGGTTTACATCACCGCGAAGGTCAGCAGATATTGCACATCAGAGACGGCGAGCGTGCCGCGTGGGGCGATAAAAAAGAGATCGACACTCACTACATTAATGACGGTGGCAACCACTGAAGGCCAGCGTCCAt                                                                 >  1:166015/1‑251 (MQ=255)                                                                                                                                               cTTTCGACATTTCATATAAGTGCCGTGTGCGTTGCTCGCGGTAACGGGCTACCCGCGTCTGATAACGCACGCCAGCAGTAAGGTGCCCGATCACCAGCCCGACgg                                                                                                                                                                                                  <  1:82019/105‑1 (MQ=255)                                                                                                                                                       aTTTCATATAAGTGCCGTGTGCGTTGCTCGCGGTAACGGGCTACCCGCGTCTGATAACGCACGCCAGCAGTAAGTGTCCCGATCACCAGCCCGACGGTTAACATCACCGCGAAGTTCAGCAGATATTGCACATCAGAGACGGCGAGCGTGCcgc                                                                                                                                         <  1:222114/154‑1 (MQ=255)                                                                                                                                                                                             ggCTACCCGCGTCTGATAACGCACGCCAGCAGTAATGTTCCCGATCACCAGCCCGACGGTTAACATCACCGCGAAGGTCAGCAGATATGGCACATCAGAGACGGCGAGCGTGCCGCGTGGGGCGATAAAAAAGAGATCGAAACTCACTACATTAATGACGGTGGCAACCACTGAAGGCCAGCGTCCATAAAATAGCGCCACCACCACCACGCCAAGCAGATACAGCATCACCAGGTTGGCGGCATCAAACg  <  1:215870/251‑1 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                                                                GTCATTCCTTGCGGATGTGTTAACGGCTGCAATTTACCGTTGTCATCGGGCAACAACACCTGACTGCGGGCATGAAACGTGGAGGCAATAAATTGTTCGCTGGTGGCAGCGATATCCTGCGGACTGCGGCCCACCGCCAGAGCTTTCGACATTTCATATAAGTGCCGTGTGCGTTGCTCGCGGTAACGGGCTACCCGCGCCTGATAACGCACGCCAGCAGTAAGGTTCCCGATCACCAGCCCGACGGTTAACATCACCGCGAAGGTCAGCAGATATTGCACATCAGAGACGGCGAGCGTGCCGCGTGGGGCGATAAAAAAGAGATCGAAACTCACTACATTAATGACGGTGGCAACCACTGAAGGCCAGCGTCCATAAAATAGCGCCACCACCACCACGCCAAGCAGATACAGCATCACCAGGTTGGCGGCATCAAACG  >  NC_000913/722712‑723150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑